High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source

Kekez, Branka; Gojgić-Cvijović, Gordana; Jakovljević, Dragica; Kojić, Jovana R. Stefanović; Marković, Marijana; Beškoski, Vladimir; Vrvić, Miroslav M.

doi:10.1007/s12010-015-1475-8

Cited by 38 publications

(19 citation statements)

References 35 publications

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“…Increasing the proportion of molasses in the medium to a sucrose in molasses:commercial sucrose ratio of 160:40 or greater inhibited levan synthesis, independently of the addition of phosphate. Our previous study has shown that for B. licheniformis NS032, the maximum productivity (0.26-0.29 g levan/g available sucrose) was achieved in BM with 100-200 g/L sucrose [14].…”

Section: Accepted Manuscriptmentioning

confidence: 92%

“…Previously, our research group reported efficient, high levan production by B. licheniformis NS032 in media with 200 and 400 g/L sucrose using an inorganic nitrogen source [14]. However, considering its high sucrose content, molasses would seem to be a natural alternative to synthetic media for levan biosynthesis.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…x 7H 2 O 0.001) with 200 and 400 g/L sucrose, 2.4 and 4.6 g/L NH 4 Cl and initial pH 7.0 and 7.4, respectively, according to our previously reported procedure [14].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…1. Control of pH value has proved to be one of the most important factors in optimizing the fermentation medium, not only for B. licheniformis NS032, but also for other levan producers [14,27,28,29].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium

Gojgić-Cvijović

Jakovljević

Lončarević

et al. 2019

International Journal of Biological Macromolecules

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The production of levan by Bacillus licheniformis NS032 in a medium based on sugar beet molasses was studied. High polysaccharide yields were produced by using diluted molasses (100-140 g/L of total sugars) with the addition of commercial sucrose up to 200 g/L of total sugars, as well as K 2 HPO 4. A levan yield of 53.2 g/L was obtained on a medium optimized by response surface methodology, containing 62.6% of sugar originating from molasses, and 4.66 g/L of phosphate, with initial pH value of 7.2. In comparison to the media with 200 and 400 g /L sucrose, in the molasses optimized medium, the observed bacterial growth was faster, while the maximum

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Section: Accepted Manuscriptmentioning

confidence: 92%

Section: Accepted Manuscriptmentioning

confidence: 99%

“…x 7H 2 O 0.001) with 200 and 400 g/L sucrose, 2.4 and 4.6 g/L NH 4 Cl and initial pH 7.0 and 7.4, respectively, according to our previously reported procedure [14].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Section: Accepted Manuscriptmentioning

confidence: 99%

See 2 more Smart Citations

Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium

Gojgić-Cvijović

Jakovljević

Lončarević

et al. 2019

International Journal of Biological Macromolecules

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“…Levan, a microbial polysaccharide, was prepared by cultivating Bacillus licheniformis NS032 in sucrose medium. The isolation, purification and physicochemical characterization of the polysaccharide are detailed in Kekez et al 34…”

Section: Microbial Biosynthesis Of Levanmentioning

confidence: 99%

Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating

Milenković¹,

Radotić²,

Matović

et al. 2018

JSCS

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Cerium oxide (CeO 2) nanoparticles (CONPs) are interesting biomaterials with various applications in biomedicine, cosmetics and the pharmaceutical industry, but with limited practical application because of their low stability in aqueous media. The aim of this study was to obtain CONPs with increased stability by coating the particles. Microbial exopolysaccharides (levan, pullulan) and glucose were used to prepare CONPs under different synthesis conditions. Coating was attempted by adding the carbohydrates during (direct coating) or after (subsequent coating) the synthesis of CONPs. The obtained nanoparticles were characterized by X-Ray diffraction analysis, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The suspension stability of the uncoated and coated CONPs in aqueous media was evaluated by measuring the hydrodynamic size, zeta potential and turbidity. The FT-IR spectra revealed the differences between coated CONPs and showed the success of subsequent coating with carbohydrates. Coating with carbohydrates improved the stability the CONP suspension by decreasing the size of aggregated particles. The suspensions of levan-and glucose-coated CONPs had the best stability. In this study, CONPs were prepared using non-toxic materials, which were completely environmentally friendly. The obtained results open new horizons for CONP synthesis, improving their biological applications.

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Biopolymer and polymer precursor production by microorganisms: applications and future prospects

Saharan,

Kamal,

Badoni

et al. 2023

J of Chemical Tech & Biotech

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Polymer has been used in various industries over the past few decades due to its tremendous applications. Among these, polyhydroxyalkanoates (PHA) and polylactic acid (PLA), are easily biodegradable biopolymers derived from bacteria, including recombinant Escherichia coli, Alcaligenes eutrophus, Alcaligenes latus, Azotobacter vinelandii, methylotrophs, and Pseudomonas. Conventional petroleum‐derived polymers have become potentially harmful to the environment due to their complex degradation process. The non‐biodegradability of synthetic polymers has become a global issue of concern. There is an urgent need for a substitute to tackle the increasing environmental stress. Microorganisms are small factories for producing different types of polymers during their growth cycle. The various features like biodegradability, biocompatibility, non‐toxicity, and wide substrate spectrum make the microbial polymers highly reliable. Biopolymers such as alginate, cellulose, cyanophycin, levan, poly‐hydroxyalkanoates, xanthan, poly‐lactic acid, and poly‐γ‐glutamic acid can be obtained from different microorganisms like Aureobasdium pullulans, Acetobacter xylinum, Bacillus thermoamylovorans, Cupriavidusnecator. These are extensively used in various fields like food, medicine, wastewater treatment, biofuel production, packaging, and cosmetics. Despite being advantageous in several ways, the biopolymer market still faces several hurdles. This review mainly emphasizes the different types of biopolymers, production by microorganisms and various applications of these biopolymers in different fields. The main drawback limiting the development of these polymers is the high production cost and low efficiency of the microbial strains. Genetic recombination is an efficient technique to enhance the microbial yield and to expand the biopolymers market size.This article is protected by copyright. All rights reserved.

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High Levan Production by Bacillus licheniformis NS032 Using Ammonium Chloride as the Sole Nitrogen Source

Cited by 38 publications

References 35 publications

Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium

Production of levan by Bacillus licheniformis NS032 in sugar beet molasses-based medium

Improving stability of cerium oxide nanoparticles by microbial polysaccharides coating

Biopolymer and polymer precursor production by microorganisms: applications and future prospects

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