High interleukin 12 and low interleukin 10 production after in vitro stimulation detected in sepsis survivors

Stanilova, Spaska; Karakolev, Z; Dimov, Gospodin S.; Dobreva, Zlatka Georgieva; Miteva, Lyuba; Slavov, E.; Stefanov, Chavdar Stefanov; Stanilov, Noyko

doi:10.1007/s00134-005-2575-7

Cited by 41 publications

(30 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The IL-12 values were lower than some have reported, 19 but consistent with other reports. 20 Laboratory measurement error is unlikely here since each coefficient of variability (CV) was lower than 10%, and because each member of the triad was run on the same ELISA plate, the comparisons were internally valid.…”

Section: Discussionsupporting

confidence: 92%

Interleukin-2, interleukin-12, and interferon-γ levels and risk of young adult Hodgkin lymphoma

Cozen

Gill

Salam

et al. 2008

Blood

View full text Add to dashboard Cite

Young adult Hodgkin lymphoma (YAHL)is associated clinically with altered immunity, including a systemic defect in cellmediated responses. There is strong evidence of a genetic contribution to risk, so we hypothesized that heritable alterations in cytokine production associated with Th1 function may contribute to susceptibility. We identified twin pairs in whom at least one member had YAHL and measured interleukin-2 (IL-2), interleukin-12 (IL-12), and interferon-␥ (IFN-␥) levels in PHA-stimulated peripheral blood mononuclear cell supernatant in 90 case-twins, 84 of their disease-free twins (unaffected cotwins), and 90 matched controls. Mean difference and mean percentage difference in cytokine levels between casetwins and controls, and unaffected cotwins and controls were determined using analysis of covariance. YAHL casetwins and their unaffected cotwins had IL-12 levels that were 60.6% (P ‫؍‬ .002) and 49% (P ‫؍‬ .04) lower than those of their matched controls, respectively. IL-2 levels were significantly higher in casetwins (P ‫؍‬ .049), but not unaffected cotwins (P ‫؍‬ .57), compared with controls. Differences in IFN-␥ levels were not statistically significant in either comparison. An IL-12 polymorphism known to regulate expression was associated with a 2.8-fold (P ‫؍‬ .03) increase in YAHL risk. Thus, both case-twins and their unaffected cotwins had a decreased ability to produce IL-12, which may contribute to YAHL susceptibility. IntroductionHodgkin lymphoma is characterized by 3 distinctive age-specific incidence peaks associated with different demographic factors, probably representing distinct etiologies. 1 Young adult Hodgkin lymphoma (YAHL), diagnosed approximately from ages 13 to 45 years, consists mainly of an Epstein-Barr virus (EBV)-negative, nodular sclerosis subtype, and has been historically strongly (positively) correlated with higher socioeconomic status and low sibship size. 2 These factors suggest an association between early childhood isolation from infection and risk, consistent with delayed exposure to a common childhood infection ("polio model"). 3 Although Epstein-Barr virus infection would be consistent with this set of factors, the best study to date found no increased risk of EBV-negative Hodgkin lymphoma, the most common type of YAHL, among 40 000 persons with laboratory-documented infectious mononucleosis. 4 (The same study did demonstrate a 20-fold risk of EBV-positive Hodgkin lymphoma within 5 years of the infectious mononucleosis diagnosis, but EBV-positive Hodgkin lymphoma accounts for only 10% to 25% of the disease in young adults.)Genetic factors also contribute to YAHL susceptibility. Excess risk has been reported in siblings 5 and family members 6 of cases, and we found a greater excess risk in identical (monozygotic, MZ) cotwins compared with fraternal (dizygotic, DZ) cotwins of cases. 7 An immune response phenotype associated with altered cytokine levels constitutes a likely candidate for a heritable risk factor since the pathological and clinical hallmark of Hodgkin ly...

show abstract

Section: Discussionsupporting

confidence: 92%

Interleukin-2, interleukin-12, and interferon-γ levels and risk of young adult Hodgkin lymphoma

Cozen

Gill

Salam

et al. 2008

Blood

View full text Add to dashboard Cite

show abstract

“…[16] Impaired pre-operative monocyte IL-12 secretion was found in patients who developed fatal postoperative sepsis. [17] These results are similar to those of Stanilova's study, [18] which showed that survivors with severe sepsis produce more IL-12 from LPS-stimulated PBMCs than non-survivors. The main immunological function of IL-12 is to enhance native T lymphocyte differentiation to Th1 cells.…”

Section: Discussionsupporting

confidence: 85%

Glucose increases interleukin-12 gene expression and production in stimulated peripheral blood mononuclear cells of type 2 diabetes patients

Chu¹,

Kuo²,

Hua³

et al. 2014

Biomed J

View full text Add to dashboard Cite

“…These tests represent reliable methods to assess the phenomenon of endotoxin tolerance defined as a reduced responsiveness to a secondary LPS challenge following a first inflammatory response. Monocytes from septic patients usually present with a diminished capacity to release tumor necrosis factor (TNF)-α, IL-1α, IL-6, and IL-12, whereas the release of anti-inflammatory mediators (IL-1ra, IL-10) is not affected or even slightly increased (33)(34)(35)(36)(37)(38). This shows that LPS can still activate monocytes but that the intracellular signaling has been turned to favor production of anti-inflammatory molecules, therefore supporting the concept of leukocyte reprogramming (39,40).…”

Section: Functional Testingmentioning

confidence: 99%

Monitoring Immune Dysfunctions in the Septic Patient: A New Skin for the Old Ceremony

Monneret

Venet

Pachot

et al. 2008

Mol Med

298

323

View full text Add to dashboard Cite

High interleukin 12 and low interleukin 10 production after in vitro stimulation detected in sepsis survivors

Abstract: The viability of PBMC at the onset of sepsis and enhanced production of IL-12 and diminished production of IL-10 after stimulation with all stimuli used may be a favorable prognostic factor in sepsis.

Cited by 41 publications

References 33 publications

Interleukin-2, interleukin-12, and interferon-γ levels and risk of young adult Hodgkin lymphoma

Interleukin-2, interleukin-12, and interferon-γ levels and risk of young adult Hodgkin lymphoma

Glucose increases interleukin-12 gene expression and production in stimulated peripheral blood mononuclear cells of type 2 diabetes patients

Monitoring Immune Dysfunctions in the Septic Patient: A New Skin for the Old Ceremony

Contact Info

Product

Resources

About