We have developed a new micro x‐ray fluorescence spectrometry (µ‐XRF) instrument using polycapillary x‐ray lens (PCXL) combined with glass conical pinhole (GCP). The x‐rays focused by the PCXL were focused again by the GCP. The size of the obtained x‐ray microbeam was estimated by a wire scanning method. In our instrument, the size of the x‐ray microbeam end was 25 µm for Cr Kα at 0.5 mm from the GCP. The divergence angle of x‐ray microbeam was 15 mrad. In order to evaluate the performance of our instrument, the gain (x‐ray flux density) was estimated. The gain in our instrument was corrected by comparison of the intensity per unit area for a Cu pinhole aperture. The gain thus obtained was approximately 1400. This highly intense micro x‐ray beam will be useful for micro‐XRF analysis. Copyright © 2009 John Wiley & Sons, Ltd.