High infection rate of bank voles (<i>Myodes glareolus</i>) with Puumala virus is associated with a winter outbreak of haemorrhagic fever with renal syndrome in Croatia

Tadin, Ante; Bjedov, Linda; Margaletić, Josip; Zibrat, Branka; Krajinović, Lidija Cvetko; Svoboda, Petra; Kurolt, Ivan-Christian; Majetić, Zrinka Štritof; Turk, Nenad; Rode, Oktavija Đaković; Čivljak, Rok; Kuzman, Ilija; Markotić, Alemka

doi:10.1017/s095026881300321x

Cited by 12 publications

(10 citation statements)

References 23 publications

(27 reference statements)

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“…Increasing altitude is generally known to decrease abundance, mortality and transmission of pathogens (LaPointe et al, 2010;Zanet et al, 2017;van Riper et al, 1986;Michel et al, 2014). While some studies have found positive correlations between pathogen (Yersinia pestsis and viruses) prevalence in rodent hosts and altitude (Tadin et al, 2014;Tollenaere et al, 2010;Tollenaere et al, 2008;Tollenaere et al, 2011), other studies have reported no correlation between altitude and ectoparasite (flea) abundance, but found positive correlations between the diversity of ectoparasites and altitude (Moore et al, 2015). In the present study, we also found no relationship between gastrointestinal parasite prevalence, abundance and intensity, and altitude.…”

Section: Discussioncontrasting

confidence: 73%

Altitudinal Effects on Innate Immune Response of a Subterranean Rodent

et al. 2020

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Section: Discussioncontrasting

confidence: 73%

Altitudinal Effects on Innate Immune Response of a Subterranean Rodent

et al. 2020

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“…In addition, SAAV and DOBV-Kurkino genome sequences were also found in rodents, but so far, no human cases have been associated with these viruses in Croatia [18,37]. Moreover, a high infection rate of bank voles with PUUV (77.4%) was documented on the Medvednica mountain near Zagreb during the 2012 outbreak [17].…”

Section: Discussionmentioning

confidence: 99%

“…Both PUUV and, to a lesser extent, DOBV have been found to circulate among humans in Croatia [12]. Besides PUUV and DOBV, SAAV is also detected in rodents [16][17][18]. In the 1990s, 1.6% of forest workers and 5.4% of the general population from the endemic territory of Lika and Gorski Kotar were seropositive for hantaviruses.…”

Section: Introductionmentioning

confidence: 99%

Clinical and virological characteristics of hantavirus infections in a 2014 Croatian outbreak

Vilibić-Čavlek

Furic²,

Barbić

et al. 2017

J Infect Dev Ctries

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Introduction: Croatia is endemic for hemorrhagic fever with renal syndrome (HFRS), with both Puumala (PUUV) and Dobrava virus (DOBV) documented. Several large outbreaks were recorded in 1995, 2002, and 2012. We analyzed demographic, clinical, laboratory, and virological characteristics of HFRS cases detected in three geographically close natural foci (Ogulin, Slunj, and the Plitvice Lakes surroundings) during the 2014 outbreak. Methodology: From January to December 2014, 122 patients with suspected HFRS were tested for hantavirus IgM/IgG antibodies using an indirect immunofluorescence assay (IFA). Cross-reactive samples were further tested using a western blot (WB). For hospitalized patients from Ogulin area, clinical and laboratory data were analyzed. Results: Acute infection was documented in 57 (46.7%) patients, of whom 75.4% were hospitalized. Ten (8.2%) patients were found to be IgG seropositive. Patients were 15-69 years of age and predominantly male (74.5%). The outbreak started in winter months, with most cases recorded from May to July (80.7%). The most frequently reported symptoms were fever (96.3%), chills/shivering (62.9%), and lumbar pain (48.1%). Mild clinical form was found in 66.7% patients, moderate in 18.5%, and severe in 14.8% patients (all but one infected with PUUV). One patient died. Using IFA, 48.8% patients showed monotypic antibody response, while in 51.2%, cross-reactive antibodies were found. PUUV was confirmed in 94.7% and DOBV in 5.3% HFRS cases by WB. Conclusions: Central mountainous Croatian regions are still highly endemic areas for HFRS. A higher percentage of severe PUUV infections could be at least partly associated with a patient's immune status.

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“…The name hantavirus derives from the prototype virus, Hantaan virus, which was discovered in the early 1950s during the Korean war, when troops stationed by the Hantaan river developed hemorrhagic manifestations [5]. Outbreaks of hantavirus disease of varying severity have occurred periodically in the last decades throughout the Americas [6, 7] and in Europe and Asia [8, 9]. It is therefore important to understand the structural organization of hantavirus particles as a step forward in attempts to devise curative or preventative strategies.…”

Section: Introductionmentioning

confidence: 99%

Mechanistic Insight into Bunyavirus-Induced Membrane Fusion from Structure-Function Analyses of the Hantavirus Envelope Glycoprotein Gc

et al. 2016

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Hantaviruses are zoonotic viruses transmitted to humans by persistently infected rodents, giving rise to serious outbreaks of hemorrhagic fever with renal syndrome (HFRS) or of hantavirus pulmonary syndrome (HPS), depending on the virus, which are associated with high case fatality rates. There is only limited knowledge about the organization of the viral particles and in particular, about the hantavirus membrane fusion glycoprotein Gc, the function of which is essential for virus entry. We describe here the X-ray structures of Gc from Hantaan virus, the type species hantavirus and responsible for HFRS, both in its neutral pH, monomeric pre-fusion conformation, and in its acidic pH, trimeric post-fusion form. The structures confirm the prediction that Gc is a class II fusion protein, containing the characteristic β-sheet rich domains termed I, II and III as initially identified in the fusion proteins of arboviruses such as alpha- and flaviviruses. The structures also show a number of features of Gc that are distinct from arbovirus class II proteins. In particular, hantavirus Gc inserts residues from three different loops into the target membrane to drive fusion, as confirmed functionally by structure-guided mutagenesis on the HPS-inducing Andes virus, instead of having a single “fusion loop”. We further show that the membrane interacting region of Gc becomes structured only at acidic pH via a set of polar and electrostatic interactions. Furthermore, the structure reveals that hantavirus Gc has an additional N-terminal “tail” that is crucial in stabilizing the post-fusion trimer, accompanying the swapping of domain III in the quaternary arrangement of the trimer as compared to the standard class II fusion proteins. The mechanistic understandings derived from these data are likely to provide a unique handle for devising treatments against these human pathogens.

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High infection rate of bank voles (Myodes glareolus) with Puumala virus is associated with a winter outbreak of haemorrhagic fever with renal syndrome in Croatia

Cited by 12 publications

References 23 publications

Altitudinal Effects on Innate Immune Response of a Subterranean Rodent

Altitudinal Effects on Innate Immune Response of a Subterranean Rodent

Clinical and virological characteristics of hantavirus infections in a 2014 Croatian outbreak

Mechanistic Insight into Bunyavirus-Induced Membrane Fusion from Structure-Function Analyses of the Hantavirus Envelope Glycoprotein Gc

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