High IFN-γ production by CD8+ T cells and early sensitization among infants at high risk of atopy

Rowe, Julie; Heaton, Tricia; Kusel, Merci; Suriyaarachchi, Devinda; Serralha, Michael; Holt, Barbara J.; Klerk, Nicholas de; Sly, Peter D.; Holt, Patrick G.

doi:10.1016/j.jaci.2003.12.585

Cited by 72 publications

(54 citation statements)

References 39 publications

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“…Alternatively, SAE-IgE may be a surrogate for other covert aspects of the Th2-associated response to SAE, including the effects of SAE on polyclonal stimulation of IgE production and/or on IgE independent mechanisms. It is pertinent to note earlier findings that T-cells from a subset of atopic children are hyperresponsive to stimulation with the archetypal SEB [27,28], which triggers cytokines including IL-5, IL-10 and IL-13. Accordingly, we included assessment of PBMC responses to SEB as part of the immunophenotyping of this cohort.…”

Section: Discussionmentioning

confidence: 99%

Th2-associated immunity to bacteria in teenagers and susceptibility to asthma

Hollams

Hales²,

Bachert³

et al. 2010

European Respiratory Journal

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Bacterial colonisation of the airways is associated with increased risk of childhood asthma. Immunoglobulin (Ig)E against bacterial antigens has been reported in some asthmatics, suggesting a role for bacterial-specific type-2 immunity in disease pathogenesis. We aimed to investigate relationships between bacterial-specific IgE amongst teenagers and asthma susceptibility.We measured titres of IgE against Haemophilus influenzae, Streptococcus pneumoniae and Staphylococcus aureus in 1,380 teenagers, and related these to asthma symptomatology and immunophenotypes.IgE titres against S. aureus-derived enterotoxins were highest amongst atopics and were associated with asthma risk. Surprisingly, IgE titres against H. influenzae and S. pneumoniae surface antigens were higher, not stratified by atopy and independently associated with decreased asthma risk.The positive association between type-2 immunity to S. aureus and asthma phenotypes probably reflects IgE-mediated effector cell activation via enterotoxin super antigens which are secreted in soluble form. The contrasting benign nature of type-2 immunity to H. influenzae and S. pneumoniae antigens may reflect their lower availability in soluble forms that can crosslink IgE receptors. We theorise that instead they may be processed by antigen presenting cells and presented to type-2 memory cells leading to mucosal secretion of interleukin (IL)-4/IL-13, a mechanism widely recognised in other tissues to attenuate T-helper-1 associated bacterialinduced inflammation.

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Section: Discussionmentioning

confidence: 99%

Th2-associated immunity to bacteria in teenagers and susceptibility to asthma

Hollams

Hales²,

Bachert³

et al. 2010

European Respiratory Journal

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“…We previously reported preliminary findings of an association between cesarean delivery and increased levels of interleukin (IL-) 13 and interferon gamma in cord blood 44 , which have in turn been associated with an increased risk for the development of atopy or asthma in childhood [45][46][47][48] . Thus, cesarean delivery may affect early neonatal immune responses by mechanisms other than alteration of the neonatal gut flora.…”

Section: Discussionmentioning

confidence: 99%

Birth by cesarean section, allergic rhinitis, and allergic sensitization among children with a parental history of atopy

Pistiner

Gold

Abdulkerim

et al. 2008

Journal of Allergy and Clinical Immunology

146

104

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“…Cryopreserved MNCs were thawed rapidly at 37 °C, resuspended in RPMI 1640, centrifuged, and resuspended to a total volume (vol) of 1 ml in AIM-V (Gibco) supplemented with 2-mercaptoethanol (2-ME, Sigma, Sydney, Australia), as described (42). Experimental conditions were optimized for bacterial dose and time of cytokine production, using MNCs from all age groups.…”

Section: Mnc Culturesmentioning

confidence: 99%

“…Cytokines were measured using commercial enzyme-linked immunosorbent assay (for IL1β, IL8; eBioscience, San Diego, CA) or in-house time-resolved fluorometry assay (for IL6, IL10, TNF-α) in accordance with the manufacturer's instructions or established protocols (42).…”

Section: Cytokine Assaysmentioning

confidence: 99%