HIGH HYDROSTATIC PRESSURE INDUCES SYNTHESIS OF HEAT‐SHOCK PROTEINS AND TREHALOSE‐6‐PHOSPHATE SYNTHASE IN Anastrepha ludens LARVAE

Abstract

The Mexican fruit fly (Anastrepha ludens) is responsible for losses of up to 25% of crops such as mango and citrus fruits in Central America and México. The larval life cycle of A. ludens comprises three stages with a duration ranging from 3 to 8 days. Because of the damage caused by A. ludens, several methods of control have been studied and implemented. High hydrostatic pressures (HHP) are currently applied to foods and it is now proposed to be employed to inactivate eggs and larvae of A. ludens. Originally HHP was designed to inactivate microorganisms, since it exerts marked effects on cell morphology, and can affect enzymatic reactions and genetic mechanisms of microbial cells, with no major changes altering the sensory or nutritional quality of the foodstuff. In this study, A. ludens in two larval stages (5- and 8-day-old) were subjected to HHP treatments. The biochemical response of the larvae of A. ludens was dependent on their stage of development. The third larval stage (L3) developed a better protection mechanism based on the synthesis of stress proteins or heat-shock proteins (HSPs) and the enzyme trehalose-6-phosphate synthase, which are linked and possibly act together to achieve greater survivability to stress caused by hydrostatic pressure.

Invertebrate Trehalose-6-Phosphate Synthase Gene: Genetic Architecture, Biochemistry, Physiological Function, and Potential Applications

“…Anastrepha ludens larvae developed a protection mechanism based on the synthesis of trehalose by TPS to achieve greater survivability to stress caused by hydrostatic pressure (Vargas-Ortiz et al, 2013 ). Starvation and the injection of dsSeHTF—an Akh-like hypertrehalosemic factor—can significantly decrease TPS expression level (Park and Kim, 2017 ).…”

Precooling Treatments Induce Resistance of <I>Anastrepha ludens</I> Eggs to Quarantine Treatments of High-Pressure Processing Combined With Cold

“…The small HSP that were less expressed compared with the HSP70 family, had a modest 10-fold increase in expression, but they are important in the hightemperature response (Denlinger and Yocum 1998) and seemed to be associated with the resistance of some individuals to HPP (Castañ ó n-Rodrṍguez et al 2011). Recently, it has been found that HPP induced the expression of HSP and enzyme trehalose-6-phosphate synthase in the larvae of A. ludens, which are associated with a greater survivability to stress caused by HPP (Vargas-Ortiz et al 2013b). It seems that HSP, or by analogy cold shock proteins, could be responsible for the resistance of A. ludens to precooling followed by HPP.…”