High-grade glioma associated immunosuppression does not prevent immune responses induced by vaccination with autologous, tumor-lysate pulsed dendritic cells

Technau, Antje; Freitag, Benjamin; Löhr, Matthias; Hagemann, Carsten; Rachor, Johannes; Keupp, Anne; Stein, Ulrike; Monoranu, Camelia‐Maria; Kessler, Amy L; Krauß, Jürgen; Wölfl, Matthias; Ernestus, RI; Gierlich, P; Engelhardt, Sabrina; Gelbrich, Götz; Schlegel, PG; Eyrich, Matthias

doi:10.1055/s-0037-1607407

Klinische Pädiatrie

2017

DOI: 10.1055/s-0037-1607407

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High-grade glioma associated immunosuppression does not prevent immune responses induced by vaccination with autologous, tumor-lysate pulsed dendritic cells

Antje Technau

Benjamin Freitag

Matthias Löhr

et al.

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“…Poster presentation [ 1 ]. 26th Annual Meeting of the working group “Experimental Neuro-Oncology”, Apr 29–30, 2017, University Hospital for Children and Adolescents, Johannes Wesling Klinikum Minden, Ruhr University Hospital Bochum, Germany, oral presentation [ 2 ].…”

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High-grade glioma associated immunosuppression does not prevent immune responses induced by therapeutic vaccines in combination with Treg depletion

Löhr¹,

Freitag

Technau

et al. 2018

Cancer Immunol Immunother

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High-grade gliomas (HGG) exert systemic immunosuppression, which is of particular importance as immunotherapeutic strategies such as therapeutic vaccines are increasingly used to treat HGGs. In a first cohort of 61 HGG patients we evaluated a panel of 30 hematological and 34 plasma biomarkers. Then, we investigated in a second cohort of 11 relapsed HGG patients receiving immunomodulation with metronomic cyclophosphamide upfront to a DC-based vaccine whether immune abnormalities persisted and whether they hampered induction of IFNγ+ T-cell responses. HGG patients from the first cohort showed increased numbers of leukocytes, neutrophils and MDSCs and in parallel reduced numbers of CD4+/CD8+ T-cells, plasmacytoid and conventional DC2s. MDSCs and T-cell alterations were more profound in WHO IV° glioma patients. Moreover, levels of MDSCs and epidermal growth factor were negatively associated with survival. Serum levels of IL-2, IL-4, IL-5 and IL-10 were altered in HGG patients, however, without any impact on clinical outcome. In the immunotherapy cohort, 6-month overall survival was 100%. Metronomic cyclophosphamide led to > 40% reduction of regulatory T cells (Treg). In parallel to Treg-depletion, MDSCs and DC subsets became indistinguishable from healthy controls, whereas T-lymphopenia persisted. Despite low T-cells, IFNγ-responses could be induced in 9/10 analyzed cases. Importantly, frequency of CD8+VLA-4+ T-cells with CNS-homing properties, but not of CD4+ VLA-4+ T-cells, increased during vaccination. Our study identifies several features of systemic immunosuppression in HGGs. Metronomic cyclophosphamide in combination with an active immunization alleviates the latter and the combined treatment allows induction of a high rate of anti-glioma immune responses.Electronic supplementary materialThe online version of this article (10.1007/s00262-018-2214-0) contains supplementary material, which is available to authorized users.

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mentioning

confidence: 99%

High-grade glioma associated immunosuppression does not prevent immune responses induced by therapeutic vaccines in combination with Treg depletion

Löhr¹,

Freitag

Technau

et al. 2018

Cancer Immunol Immunother

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High-grade glioma associated immunosuppression does not prevent immune responses induced by vaccination with autologous, tumor-lysate pulsed dendritic cells

Cited by 1 publication

References 0 publications

High-grade glioma associated immunosuppression does not prevent immune responses induced by therapeutic vaccines in combination with Treg depletion

High-grade glioma associated immunosuppression does not prevent immune responses induced by therapeutic vaccines in combination with Treg depletion

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