High Glucose Concentration Promotes Vancomycin-Enhanced Biofilm Formation of Vancomycin-Non-Susceptible Staphylococcus aureus in Diabetic Mice

Hsu, Chi-Yu; Shu, Jinian; Lin, Mei-Hui; Chong, Kowit-Yu; Chen, Chien‐Cheng; Wen, Shumin; Hsieh, Yi‐Ting; Liao, Wan-Chun

doi:10.1371/journal.pone.0134852

Cited by 16 publications

(18 citation statements)

References 38 publications

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“…Pioneering groups in this field have shown that in db/db mice (a model of diabetic dyslipidemia), Pseudomonas aeruginosa or S. aureus biofilms delayed wound healing, and that the diabetic condition slowed down healing and increased the biofilm thickness [ 36 , 37 ]. Hsu et al also reported that high glucose levels encourage the formation of vancomycin-resistant S. aureus biofilms [ 38 ]. Other studies have shown that the host response and neutrophil oxidative burst activity were decreased in the wound, and that oxidative stress and reactive oxygen species promoted biofilm appearance [ 39 , 40 ].…”

Section: Overview Of Biofilms In Dfumentioning

confidence: 99%

Biofilms in Diabetic Foot Ulcers: Significance and Clinical Relevance

et al. 2020

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Foot infections are the main disabling complication in patients with diabetes mellitus. These infections can lead to lower-limb amputation, increasing mortality and decreasing the quality of life. Biofilm formation is an important pathophysiology step in diabetic foot ulcers (DFU)—it plays a main role in the disease progression and chronicity of the lesion, the development of antibiotic resistance, and makes wound healing difficult to treat. The main problem is the difficulty in distinguishing between infection and colonization in DFU. The bacteria present in DFU are organized into functionally equivalent pathogroups that allow for close interactions between the bacteria within the biofilm. Consequently, some bacterial species that alone would be considered non-pathogenic, or incapable of maintaining a chronic infection, could co-aggregate symbiotically in a pathogenic biofilm and act synergistically to cause a chronic infection. In this review, we discuss current knowledge on biofilm formation, its presence in DFU, how the diabetic environment affects biofilm formation and its regulation, and the clinical implications.

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Section: Overview Of Biofilms In Dfumentioning

confidence: 99%

Biofilms in Diabetic Foot Ulcers: Significance and Clinical Relevance

et al. 2020

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“…For example, TSB (which already contains 2.5 g/L glucose and 5.0 g/L NaCl) is often supplemented with additional glucose (0.25%, 0.4%, 0.5%, and 1.0%) [22,30,31,32,33,34]. In addition, brain heart infusion (BHI) (which already contains 2.0 g/L glucose) is also used as a culture media for S. aureus biofilm formation in vitro [35], with additional glucose supplementation (0.5%, 1.0%, and 1.5%) [36,37]. Lennox broth supplemented with different concentration of glucose (0 to 320 mg/dL in 20 mg/dL intervals) was also used to study the enhanced effect on biofilm formation by S. aureus [38].…”

Section: Introductionmentioning

confidence: 99%

Biofilm Formation by Staphylococcus aureus Clinical Isolates is Differentially Affected by Glucose and Sodium Chloride Supplemented Culture Media

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Park

Chung

et al. 2019

JCM

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Staphylococcus aureus (S. aureus) causes persistent biofilm-related infections. Biofilm formation by S. aureus is affected by the culture conditions and is associated with certain genotypic characteristics. Here, we show that glucose and sodium chloride (NaCl) supplementation of culture media, a common practice in studies of biofilms in vitro, influences both biofilm formation by 40 S. aureus clinical isolates (methicillin-resistant and methicillin-sensitive S. aureus) and causes variations in biofilm quantification. Methicillin-resistant strains formed more robust biofilms than methicillin-sensitive strains in tryptic soy broth (TSB). However, glucose supplementation in TSB greatly promoted and stabilized biofilm formation of all strains, while additional NaCl was less efficient in this respect and resulted in significant variation in biofilm measurements. In addition, we observed that the ST239-SCCmec (Staphylococcal Cassette Chromosome mec) type III lineage formed strong biofilms in TSB supplemented with glucose and NaCl. Links between biofilm formation and accessory gene regulator (agr) status, as assessed by δ-toxin production, and with mannitol fermentation were not found. Our results show that TSB supplemented with 1.0% glucose supports robust biofilm production and reproducible quantification of S. aureus biofilm formation in vitro, whereas additional NaCl results in major variations in measurements of biofilm formation.

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“…Nutrient deficient growth conditions appeared to promote biofilm formation. It was previously shown that biofilm production increased in the presence of glucose through increased matrix production ( You et al, 2014 ; Hsu et al, 2015 ). Here, we observed that the absence of glucose had statistically significant effect on irreversible adhesion.…”

Section: Discussionmentioning

confidence: 99%

Bone Environment Influences Irreversible Adhesion of a Methicillin-Susceptible Staphylococcus aureus Strain

et al. 2018

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Prosthesis and joint infections are an important threat in public health, especially due to the development of bacterial biofilms and their high resistance to antimicrobials. Biofilm-associated infections increase mortality and morbidity rates as well as hospitalization costs. Prevention is the best strategy for this serious issue, so there is an urgent need to understand the signals that could induce irreversible bacterial adhesion on a prosthesis. In this context, we investigated the influence of the bone environment on surface adhesion by a methicillin-susceptible Staphylococcus aureus strain. Using static and dynamic biofilm models, we tested various bone environment factors and showed that the presence of Mg2+, lack of oxygen, and starvation each increased bacterial adhesion. It was observed that human osteoblast-like cell culture supernatants, which contain secreted components that would be found in the bone environment, increased bacterial adhesion capacity by 2-fold (p = 0.015) compared to the medium control. Moreover, supernatants from osteoblast-like cells stimulated with TNF-α to mimic inflammatory conditions increased bacterial adhesion by almost 5-fold (p = 0.003) without impacting on the overall biomass. Interestingly, the effect of osteoblast-like cell supernatants on bacterial adhesion could be counteracted by the activity of synthetic antibiofilm peptides. Overall, the results of this study demonstrate that factors within the bone environment and products of osteoblast-like cells directly influence S. aureus adhesion and could contribute to biofilm initiation on bone and/or prosthetics implants.

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High Glucose Concentration Promotes Vancomycin-Enhanced Biofilm Formation of Vancomycin-Non-Susceptible Staphylococcus aureus in Diabetic Mice

Cited by 16 publications

References 38 publications

Biofilms in Diabetic Foot Ulcers: Significance and Clinical Relevance

Biofilms in Diabetic Foot Ulcers: Significance and Clinical Relevance

Biofilm Formation by Staphylococcus aureus Clinical Isolates is Differentially Affected by Glucose and Sodium Chloride Supplemented Culture Media

Bone Environment Influences Irreversible Adhesion of a Methicillin-Susceptible Staphylococcus aureus Strain

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