High glucose and TGF-β1 reduce expression of endoplasmic reticulum-resident selenoprotein S and selenoprotein N in human mesangial cells

Huang, Fei; Guo, Yue; Wang, Li; Jing, Lanmei; Zhao, Chen; Lu, Shemin; Fu, Rongguo; Tian, Liang

doi:10.1080/0886022x.2019.1641413

Cited by 10 publications

(7 citation statements)

References 23 publications

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“…We know that TGF-β is a multifunctional cytokine that induces tissue fibrosis and epithelial-mesenchymal transition [ 34 ]. Recent in vitro studies using lung fibroblasts and mesangial cells have revealed that ER stress caused the activation of the TGF-β pathway followed by fibrosis [ 35 – 37 ]. Our observation is in line with these findings; elevated ER stress led to an increased expression of TGF-β and thereby renal fibrosis.…”

Section: Discussionmentioning

confidence: 99%

Steatosis is involved in the progression of kidney disease in a high-fat-diet-induced non-alcoholic steatohepatitis mouse model

et al. 2022

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Chronic kidney disease (CKD) and non-alcoholic steatohepatitis (NASH) are major health issues associated with the metabolic syndrome. Although NASH is a known risk factor of CKD, the mechanisms linking these two diseases remain poorly understood. We aimed to investigate alterations in the kidney complicated with dyslipidemia in an established NASH mouse model. Male C57BL6/J mice were fed with control diet or high-fat diet (HFD), containing 40% fat, 22% fructose, and 2% cholesterol for 16 weeks. Metabolic characteristics, histological changes in the kidney, endoplasmic reticulum (ER) stress, apoptosis, and fibrosis were evaluated by histological analysis, immunoblotting, and quantitative reverse transcription-polymerase chain reaction. Levels of serum aspartate aminotransferase, alanine aminotransferase, alkali-phosphatase, total cholesterol, and urinary albumin were significantly higher in mice fed with HFD. Remarkable steatosis, glomerular hypertrophy, and interstitial fibrosis were also shown in in the kidney by leveraging HFD. Furthermore, HFD increased the mRNA expression levels of Casp3, Tgfb1, and Nfe2l2 and the protein level of BiP. We observed the early changes of CKD and speculate that the underlying mechanisms that link CKD and NASH are the induction of ER stress and apoptosis. Further, we observed the activation of Nfe2l2 in the steatosis-induced CKD mouse model. This NASH model holds implications in investigating the mechanisms linking dyslipidemia and CKD.

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Section: Discussionmentioning

confidence: 99%

Steatosis is involved in the progression of kidney disease in a high-fat-diet-induced non-alcoholic steatohepatitis mouse model

et al. 2022

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“…[ [153][154][155] Selenoprotein K (SELENOK) -Quality control within the ER Protection against ERS and oxidative injury [152] SELENOK expression downregulated by glucose [156] Selenoprotein V (SELENOV) -…”

Section: Selenium Transport and Storage Antioxidant Defensementioning

confidence: 99%

Selenium and Selenoproteins at the Intersection of Type 2 Diabetes and Thyroid Pathophysiology

Gorini

Vassalle

2022

Antioxidants

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Type 2 diabetes (T2D) is considered one of the largest global public-health concerns, affecting approximately more than 400 million individuals worldwide. The pathogenesis of T2D is very complex and, among the modifiable risk factors, selenium (Se) has recently emerged as a determinant of T2D pathogenesis and progression. Selenium is considered an essential element with antioxidant properties, and is incorporated into the selenoproteins involved in the antioxidant response. Furthermore, deiodinases, the enzymes responsible for homeostasis and for controlling the activity of thyroid hormones (THs), contain Se. Given the crucial action of oxidative stress in the onset of insulin resistance (IR) and T2D, and the close connection between THs and glucose metabolism, Se may be involved in these fundamental relationships; it may cover a dual role, both as a protective factor and as a risk factor of T2D, depending on its basal plasma concentration and the individual’s diet intake. In this review we discuss the current evidence (from experimental, observational and randomized clinical studies) on how Se is associated with the occurrence of T2D and its influence on the relationship between thyroid pathophysiology, IR and T2D.

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“…This limitation may lead to non-reproducible data. The present study aimed to evaluate the performance of ve frequently used reference genes (Actb, Hprt, Gapdh, 18S and Ppia) in renal models [18][19][20][21][22][23][24][25][26][27][28][29] and to identify the most stable ones and the optimal number of genes for normalization the expressions of target genes by RT-PCR in MCs treated with TGF-β model.…”

Section: Introductionmentioning

confidence: 99%

Determination of reference genes as a quantitative standard for gene expression analysis in mouse mesangial cells stimulated with TGF-β

Bronel

Anauate

Maquigussa

et al. 2022

Preprint

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Reverse transcription-quantitative polymerase chain reaction (RT-PCR) is the gold standard technique for gene expression analysis, but the choice of quantitative reference genes (housekeeping genes, HKG) remains challenging. Identification of the best HKG is essential for estimating the expression level of target genes. The aim of this study was to identify the best HKG for an in vitro model with mouse mesangial cells (MMCs) stimulated with 5 ng/mL of TGF-β. Candidate five HKG were selected: Actb, Hprt, Gapdh, 18S and Ppia. After quantitative expression, the best combination of these genes was analyzed in silico using six software programs. To validate the results, the best genes were used to normalize the gene expression of fibronectin, vimentin and α-SMA. In silico analysis revealed that Ppia, Gapdh and 18S were the most stable genes between the groups. GenEX software and Spearman's correlation determined that combination of Ppia and Gapdh is the best HKG pair. Validation of the HKG by normalizing fibronectin, vimentin and α-SMA were consistent with results from the literature. This study established the combination of Ppia and Gapdh as the best HKG pair for gene expression analysis by RT-PCR in this in vitro model using MMCs treated with TGF-β.

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High glucose and TGF-β1 reduce expression of endoplasmic reticulum-resident selenoprotein S and selenoprotein N in human mesangial cells

Cited by 10 publications

References 23 publications

Steatosis is involved in the progression of kidney disease in a high-fat-diet-induced non-alcoholic steatohepatitis mouse model

Steatosis is involved in the progression of kidney disease in a high-fat-diet-induced non-alcoholic steatohepatitis mouse model

Selenium and Selenoproteins at the Intersection of Type 2 Diabetes and Thyroid Pathophysiology

Determination of reference genes as a quantitative standard for gene expression analysis in mouse mesangial cells stimulated with TGF-β

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