High genetic variability in endophytic fungi from the genusDiaportheisolated from common bean (Phaseolus vulgarisL.) in Brazil

Abstract

The combined use of molecular tools such as multilocus phylogenetic approach and molecular markers, as performed in this study, is the best way to distinguish endophytic strains of Diaporthe isolated from common bean (Phaseolus vulgaris L.).

Seedborne Pathogenic Fungi in Common Bean (Phaseolus vulgaris cv. INTA Rojo) in Nicaragua

“…Diaporthe spp. are pathogens of many different plant species and cause seed rot, stem cankers, lesions, and pod blight [68], but in common beans they are simply endophytes [69]. Therefore, it is remarkable that the two isolates of Diaporthe from seedlot of ‘INTA Rojo’ caused severe disease symptoms in common bean seedlings (Fig 4).…”

“…miriciae were found associated with cankers on soybean and mung bean plants in Australia [68]. Analysis of the ITS regions alone is insufficient to identify the species in the genus Diaporthe [69]. It therefore seems warranted to further characterize the pathogenic isolates described in this study using, e.g., multilocus phylogenetic analysis [69].…”

Multiloci identification ofDiaporthefungi isolated from the medicinal plantCostus spiralis(Jacq.) Roscoe (Costaceae)

“…A total of 12 isolates were selected among those that presented the same band pattern for molecular identification with the ITS region and β‐tubulin, calmodulin and partial sequences of translation elongation factor 1‐α gene, with the protocol provided by Santos et al . (). The primer sets used for the amplification of four genes along with the PCR protocols are presented in Table .…”

“…), has often been used for the molecular identification of the genus Diaporthe (Santos et al . , ; Udayanga et al . , ; Elfar et al .…”

“…These studies have allowed identification, analysis of divergences, phylogenetic relationships and description of new species of the genus (Santos et al . , ; Udayanga et al . , ; Elfar et al .…”