High genetic diversity and recombination events of porcine astrovirus strains identified from ill and asymptomatic pigs in 2017, Hunan Province, China

Lv, Sun-Liang; Zhang, Huihui; Li, Jieyu; Hu, Wen-Qin; Song, Yating; Opriessnig, Tanja; Xiao, Chao-Ting

doi:10.1007/s11262-019-01692-w

Cited by 18 publications

(28 citation statements)

References 31 publications

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“…The results of the present study showed that PAstV-2 was circulating in ten different provinces, covering five regions of China (Figure 3), suggesting that PAstV-2 is the most widely distributed strain in China, which is supported by recent studies conducted in the provinces of Hebei, Hunan, Sichuan, and Guangxi (11,21,22,25). Previous studies have reported the existence of multiple PAstV genotypes in China.…”

Section: Genotype Distribution Of Pastv In Chinasupporting

confidence: 85%

“…Sequence analysis of the 27 RdRp genes identified in this study had nucleotide identities of 53.8-99.5%, indicating (4)(5)(6)12). PAstV-4, which was first reported in 2013, is a newly identified genotype in China that has since spread to the provinces of Hunan, Tianjin, Shanxi, Jilin, and Jiangxi (9,21,22). In addition, Lv et al (21) and Zhao et al (24) reported novel recombinant PAstV-4 strains in China.…”

Section: Phylogenetic Analysis Of Pastvmentioning

confidence: 63%

“…In the current study, a total of 27 RdRp genes were successfully sequenced from the 89 PAstV-positive diarrhea samples ( Table S2 ). The RdRp gene is widely used to classify the genotype of PAstV ( 5 , 11 , 12 , 21 – 23 ). Here, a phylogenetic tree was constructed based on the RdRp genes of the 27 identified PAstV strains and 115 reference PAstV strains.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, five PAstV strains identified in Jiangxi province exhibited three distinct genotypes (PAstV-2, PAstV-4, and PAstV-5). Previous studies reported the presence of two or more genotypes of PAstV in the same province of China, such as four different genotypes of PAstV circulating in Hunan province (21,25), while all five genotypes of PAstV were identified in Guangxi province (22), indicating a remarkable diversity of genotypes of PAstV cocirculating among pig farms in China.…”

Section: Genotype Distribution Of Pastv In Chinamentioning

confidence: 94%

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Coinfection and Genetic Characterization of Porcine Astrovirus in Diarrheic Piglets in China From 2015 to 2018

Yang

et al. 2020

Front. Vet. Sci.

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Porcine astrovirus (PAstV) is broadly distributed globally and exists as at least five distinct genotypes. PAstV, which was recently identified as an important pathogen of diarrhea in piglets, is widely distributed in China. However, few studies have investigated the coinfection and genetic characterization of PAstV in diarrheic piglets in China. In this study, 89 PAstV-positive samples were identified in 543 diarrhea samples in China from 2015 to 2018, of which 75.28% (67/89) were coinfected with three to five different porcine pathogens, while none were positive for PAstV only. Among the 543 diarrhea samples, statistical analysis showed that PAstV-induced diarrhea was potentially associated with coinfection of PEV (p < 0.01) and GARV (p < 0.01). Phylogenetic analysis showed that the 27 identified PAstV strains belong to three different genotypes and that PAstV-2 (81.48%, 22/27) was predominant in diarrheic piglets in China, followed by PAstV-4 (11.11%, 3/27) and PAasV-5 (7.41%, 2/27). Sequence analysis revealed that the 27 RdRp genes identified in this study had nucleotide homologies of 53.8-99.5%. In addition, the RdRp gene of PAstV-4 strain JL/MHK/2018/0115 harbored a unique insert of three nucleotides (GAA) as compared with other known PAstV-4 strains. Furthermore, the genotypes of PAstV varied among different geographical locations, although PAstV-2 was the most widely distributed in China. These data demonstrate that PAstV coinfection with other porcine pathogens was common and there was genetic diversity of PAstV in diarrheic piglets in China.

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Section: Genotype Distribution Of Pastv In Chinasupporting

confidence: 85%

Section: Phylogenetic Analysis Of Pastvmentioning

confidence: 63%

Section: Resultsmentioning

confidence: 99%

Section: Genotype Distribution Of Pastv In Chinamentioning

confidence: 94%

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Coinfection and Genetic Characterization of Porcine Astrovirus in Diarrheic Piglets in China From 2015 to 2018

Yang

et al. 2020

Front. Vet. Sci.

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“…In South Korea, 25/129 (19.4%) domestic pigs and 1/146 (0.7%) wild boar fecal samples were tested positive for PAstV [31]. Multiple PAstV types were identified in many cities in China [32][33][34][35][36][37]. The co-infection of different genotypes in the same pig was commonly observed, while in an individual pig a high genetic diversity was observed for viruses that belong to the same PAstV genotype [37].…”

Section: Discussionmentioning

confidence: 99%

Epidemiological analysis of Porcine Viral Diarrhea Pathogens in Local Area

Shi¹,

Li²,

Tao³

et al. 2020

Preprint

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Background Porcine viral diarrhea can cause great damage to the pig industry and high mortality to piglets. Furthermore, multiple pathogen infections and synergistic infections commonly existed in clinic. This has resulted in great difficulties in determining the main pathogenic factors, which would delay the prevention and control of diseases. Methods A total of 518 porcine stool specimens were collected from 9 pig herds in Shanghai, China from 2015 to 2017 and used for pathogen detection. A Luminex xTAG multiplex detection method was developed for the detection of 11 viral diarrhea pathogens, which allows for the simultaneous qualitative and quantitative detection of viral diarrhea pathogens in clinical samples. Results The minimum detection rate of the Luminex xTAG multiplex detection method was at least 10 times higher than the traditional PCR method. As a result, 209 (40.3%) were positive for porcine kobuvirus (PKoV), 138 (26.6%) for porcine astrovirus (PAstV), 91 (17.6%) for porcine epidemic diarrhea virus (PEDV), 78 (15.1%) for porcine sapelovirus (PSV), 68 (13.1%) for porcine sapovirus (PoSaV), 27 (5.2%) for porcine teschovirus (PTV), 25 (4.8%) for porcine deltacoronavirus (PDCoV), 17 (3.3%) for porcine rotavirus (PoRV), 16 (3.1%) for transmissible gastroenteritis virus (TGEV), 10 (1.9%) for porcine torovirus (PToV), and 9 (1.7%) for bovine viral diarrhea virus (BVDV), respectively. Furthermore, multiple infection rate of diarrhea sample was 17.57% for dual-infection, 11.58%for triple-infection, 4.63% for quadruple-infection, 0.77% for quintuple-infection, 0.58% for sextuple-infection and septuple-infection, respectively. Infection pattern of the viral diarrheal pathogens was changing, and different farm had the various diarrhea infection patterns, which proved the great importance of epidemiological surveillance and the guidance effect to clinical production. PoSaV, PoRV, PAstV, PToV and PEDV were indicated as the predominant viruses of clinical samples collected in 2017 by the quantitative analysis. Conclusions Here we provide a Luminex xTAG multiple detection method for viral diarrhea pathogen infection in clinical,which was more sensitive and specific than general multiplex PCR method. Furthermore, the surveillance confirmed the complicated infection status in China, which demonstrated the need for continuous surveillance and provided data for the prevention and control of viral diarrhea.

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Molecular detection and characterization reveals circulation of multiple genotypes of porcine astrovirus in Haryana, India

et al. 2021

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High genetic diversity and recombination events of porcine astrovirus strains identified from ill and asymptomatic pigs in 2017, Hunan Province, China

Cited by 18 publications

References 31 publications

Coinfection and Genetic Characterization of Porcine Astrovirus in Diarrheic Piglets in China From 2015 to 2018

Coinfection and Genetic Characterization of Porcine Astrovirus in Diarrheic Piglets in China From 2015 to 2018

Epidemiological analysis of Porcine Viral Diarrhea Pathogens in Local Area

Molecular detection and characterization reveals circulation of multiple genotypes of porcine astrovirus in Haryana, India

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