High genetic differentiation in the endemic and endangered freshwater fish Achondrostoma salmantinum Doadrio and Elvira, 2007 from Spain, as revealed by mitochondrial and SNP markers

Quaternary geomorphological and climatic changes associated with the diversification of Iberian freshwater fishes: The case of the genus Cobitis (Cypriniformes, Cobitidae)

“…According to our divergence time estimations, the timing of these divergences agrees with a common period of population differentiation for many Iberian freshwater fish species, which in turn coincides with the culmination of the formation of the Iberian drainage network and the Pleistocene glacial cycles (Casal‐López et al, 2017 ; Corral‐Lou et al, 2019 , 2021 ; Pais et al, 2012 ; Perea et al, 2016 ). Although geological information for the study area is scarce, several tectonic and climatic events are known to have occurred during the Quaternary near the study area, which influenced the hydrogeomorphology of the aquatic network such as the fluvial capture phenomena, the presence of paleoglaciers during the last glacial cycle, and changes in the trajectory of some rivers (Benito et al, 2003 ; Carrasco et al, 2013 , 2015 ; Goy et al, 2020 ).…”

“…Similarly, greater differentiation within the same sub‐basins than between different sub‐basins has been found for Achondrostoma salmantinum , which has a restricted distribution area that partially overlaps with that of C . vettonica (Corral‐Lou et al, 2021 ). Iberian freshwater fishes with a wider distribution range that partially or completely overlaps with that of C .…”

“…paludica ) (Table S1 ). Amplifications were performed following the protocol described by Doadrio and Perdices ( 2005 ) for MT ‐ CYB , and Corral‐Lou et al ( 2021 ) for RAG1 .…”

“…In recent years, phylogeographic and population genetics studies have taken advantage of next‐generation sequencing (NGS) technologies to broadly screen the genome at high resolution, yet some challenges remain in the analysis of NGS data such as the handling of large‐scale and complex data, the upstream process of pipeline, and the limited number of available reference genomes (McCormack et al, 2013 ; Tan et al, 2019 ). Combined analyses of single‐nucleotide polymorphisms (SNPs) with mitochondrial and nuclear markers have proven useful to provide more complete and more detailed phylogeographic and biogeographic models of the past and present relationships of populations of various species (Corral‐Lou et al, 2021 ; Mendes et al, 2019 ; Zarraonaindia et al, 2012 ).…”

Conservation and Phylogeography of Plants: From the Mediterranean to the Rest of the World

“…Trying to give importance to the phylogeography considerations, Doadrio et al [90] introduced the Operational Conservation Unit concept (OCUs), which is defined as a continuous area limited by geographical boundaries and inhabited by one or more populations sharing the same genetic pattern. Despite the simplicity of the proposal, this estimator has been scarcely used and always with fauna [91][92][93].…”