High genetic differentiation among wild populations of alien Medicago sativa in Lithuania

Vyšniauskienė, R.; Rančelienė, Vida; Patamsytė, Jolanta; Žvingila, Donatas

doi:10.2478/s11535-013-0159-4

Cited by 8 publications

(13 citation statements)

References 42 publications

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“…These correlations were stronger for invasive species (for I. parviflora r = 0.861 at RAPD loci and r = 0.941 at ISSR loci, for I. glandulifera r = 0.893 and r = 875, respectively) and weaker for I. noli-tangere (r = 0.696 at RAPD loci and r = 0.249 at ISSR loci). Significant correlations between genetic and geographic distances at RAPD and ISSR loci were found in other studies of invasive species of the other genera, like Erigeron annuus (Edwards et al, 2006;Patamsytė et al, 2013 a), although insignificant in a case of Medicago sativa, M. falcata (Vyšniauskienė et al, 2013) and Bunias orientalis (Patamsytė et al, 2013 b). Comparisons of genetically close invasive and native plants are of special importance for elucidating mechanisms of spread and forecasting this process (Hejda et al, 2009), prompting theoretically validated methods for eradication.…”

Section: Glandulifera)mentioning

confidence: 74%

“…Alien species on the way of their expansion might meet congenerous species. Few investigations deal with comparisons of geographically close relatives, local and alien ones like in the cases of genera Atriplex (Mandak, 2003) and Medicago (Vyšniauskienė et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

“…Multilocus dominant markers, like RAPD or ISSR meet such requirements and have been employed alone or together for the research of invaders of the other genera: Erigeron annuus (Edwards et al, 2006), Bunias orientalis (Patamsytė et al, 2013 b) and Medicago sativa (Vyšniauskienė et al, 2013). The present study is aimed at evaluation of populations of three Impatiens species from two countries employing two multilocus markers of different type.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of genetic diversity of three Impatiens species from Central Europe and Baltic region

Kupčinskienė

Zybartaitė

Paulauskas

2015

Zemdirbyste-Agriculture

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The present study is aimed at evaluation of genetic diversity of populations of three Impatiens species from two countries by employing multilocus markers of two different types. The total number of individuals was 355, the number of populations for each species -native Impatiens noli-tangere and invasive -I. parviflora, I. glandulifera, was 8 (4 from Lithuania and 4 from the Czech Republic). Impatiens species were evaluated according to 8 randomly amplified polymorphic DNA (RAPD) loci and 5 inter-simple sequence repeat (ISSR) loci. Independently of the site, at the species level polymorphism was the highest for I. glandulifera at RAPD loci (35.2%, as a mean per population) and for I. noli-tangere at ISSR loci (48%). RAPD and ISSR based dendrograms for individuals and populations also principal coordinate analyses clearly clustered populations of each species according to their countries, except for ISSR based dendrogram for the populations of I. noli-tangere. Estimated at ISSR loci, molecular variance among the populations of the two countries was much smaller for native I. noli-tangere (7.3%), when compared to invasive I. parviflora (49.7%) or I. glandulifera (45.6%). Genetic differentiation among the populations was the smallest for I. noli-tangere (G ST = 0.36). The populations of both invasive species from the Czech Republic were more polymorphic at ISSR loci, when compared to those from Lithuania. Mantel test showed significant correlations between geographic and genetic distances in the case of both DNA markers. The Czech Republic's populations were bigger in size, grew in higher altitudes, mean annual temperatures of the site were higher by several degrees and mean annual rainfall was lower compared to Lithuania's. Polymorphisms at RAPD and ISSR loci might play different role for viability of populations of Impatiens species. ISSR markers were more useful for the assessment of genetic differences between native and invasive Impatiens species.

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Section: Glandulifera)mentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of genetic diversity of three Impatiens species from Central Europe and Baltic region

Kupčinskienė

Zybartaitė

Paulauskas

2015

Zemdirbyste-Agriculture

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“…(Halldén et al, 1994;Riaz et al, 2001;Negi et al, 2004;Hasan et al, 2006). RAPD markers are used for genetic diversity analysis of many plant species (Galović et al, 2006;Vyšniauskienė et al, 2011;Zybartaite et al, 2011;Vyšniauskienė et al, 2013). RAPD markers were used for evaluation of genetic variability in some Brassica species (Dulson et al, 1998;Yu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Morphological traits and genetic diversity of differently overwintered oilseed rape (Brassica napus L.) cultivars

Paulauskas

Jodinskienė

Griciuvienė

et al. 2013

Zemdirbyste-Agriculture

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Winter oilseed rape is an important crop grown in many countries for its valuable oil and protein. One of the main problems of cultivating rapeseed in northern countries is winter survival. The objective of this study was to assess morphological traits and genetic diversity of eleven cultivars of winter oilseed rape (Brassica napus L.) grown in experimental fields and to determine the correlation between phenotypic variation, deoxyribonucleic acid (DNA) polymorphism and overwintering characteristics. Oilseed rape stand density, height of growth point from the soil surface, thickness of root neck as well as the number of developed leaves per plant were observed for morphological traits analysis. The number of owerwintered plants was evaluated. The genetic variability of 11 oilseed rape cultivars was evaluated by random amplified polymorphic DNA (RAPD) screening 14 primers, 9 of them being polymorphic and used for further analysis. A total of 84 reproducible RAPD bands were identified among 134 individuals, 73 of the detected DNA bands were polymorphic. Among cultivars Nei's gene diversity ranged in the interval 0.1344-0.0313, Shannon's information index was 0.2022-0.0449. An analysis of molecular variance (AMOVA) showed a significant genetic diversity among populations (Phi PT = 0.684, p < 0.01). Unweighted pair group with arithmetic mean (UPGMA) cluster analysis dendrogram generated on the basis of 73 polymorphic RAPD bands showed a clear separation of all analysed cultivars to different clusters. Dendrogram constructed using five morphological traits divided 11 oilseed rape cultivars into two clusters with one stand-alone cultivar 'DK Secure'. No significant correlation of genetic diversity with morphological traits was obtained. A strong and significant correlation between the oilseed rape overwintering capability and the number of polymorphic loci was obtained only in 2008-2009 season (r = 0.81, p = 0.03).

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“…Genetic diversity within and among alfalfa populations have been assessed by using various types of DNA markers: Random Amplified Polymorphic DNA (RAPD) [9][10][11][12], Amplified Fragment Length Polymorphism (AFLP) [13,14], Restriction Fragment Length Polymorphism (RFLP) [15,16], Simple Sequence Repeat (SSR) [17][18][19], Intersimple Sequence Repeat (ISSR) [20] and Sequence Related Amplified Polymorphisms (SRAP) [21,22]. Results of all this research lead to conclusions (i) that alfalfa populations, both wild and cultivated, are highly diverse and (ii) that most genetic variation in alfalfa resides within populations.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation and relationship of alfalfa populations and their progenies based on RAPD markers

et al. 2014

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The aim of investigation was to evaluate genetic variation and relationship among alfalfa populations and their offspring, with minimal cost, by using DNA marker analysis. RAPD analysis was performed on bulked DNA samples of five alfalfa parental populations and their progenies: 20 F1 populations from reciprocal diallel crosses and five S1 populations from self-pollination. Twenty primers generated 217 bands, ranging in size from 300 to 6000 bp, with the average number of bands per primer of 10.85 and polymorphism information content of 0.246. Percentage of polymorphic loci, effective number of alleles, expected heterozygosity and Shannon’s information index were used to estimate genetic variation. Higher diversity was observed in F1 progeny populations, while genetic variation in parental populations and S1 progenies remained similar. The genetic relatedness of alfalfa populations was analysed by UPGMA and Bayesian model-based clustering approach. In both types of analysis selfpollinated progenies were grouped. Furthermore, the hybrid offspring where Zuzana, and RSI 20 were maternal parents were placed in separate groups. The results indicate that use of RAPD markers on bulked DNA samples can be fast and cost-effective way for differentiation of alfalfa parental populations and their offspring, as well as for evaluation of their genetic relationships.

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High genetic differentiation among wild populations of alien Medicago sativa in Lithuania

Cited by 8 publications

References 42 publications

Comparison of genetic diversity of three Impatiens species from Central Europe and Baltic region

Comparison of genetic diversity of three Impatiens species from Central Europe and Baltic region

Morphological traits and genetic diversity of differently overwintered oilseed rape (Brassica napus L.) cultivars

Genetic variation and relationship of alfalfa populations and their progenies based on RAPD markers

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