High Frequency Ultrasound for the Analysis of Fetal and Placental Development &lt;em&gt;In Vivo&lt;/em&gt;

Meyer, Nicole; Schuler, Thomas M.; Zenclussen, Ana Claudia

doi:10.3791/58616-v

Cited by 3 publications

(2 citation statements)

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“…Methods adopted for Doppler ultrasound analyses of placental labyrinth and umbilical vessels were performed as previously described ( 49 ). Briefly, E14.5 dams were initially anesthetized with isoflurane at 3.5%, transferred to a heated stage to maintain 37°C body temperature in a dorsal position, and remained anesthetized affixed to an anesthesia nose tube with 1.5% isoflurane and O 2 .…”

Section: Methodsmentioning

confidence: 99%

The maternal microbiome promotes placental development in mice

Pronovost,

Yu,

Coley-O’Rourke

et al. 2023

Sci. Adv.

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The maternal microbiome is an important regulator of gestational health, but how it affects the placenta as the interface between mother and fetus remains unexplored. Here, we show that the maternal gut microbiota supports placental development in mice. Depletion of the maternal gut microbiota restricts placental growth and impairs feto-placental vascularization. The maternal gut microbiota modulates metabolites in the maternal and fetal circulation. Short-chain fatty acids (SCFAs) stimulate cultured endothelial cell tube formation and prevent abnormalities in placental vascularization in microbiota-deficient mice. Furthermore, in a model of maternal malnutrition, gestational supplementation with SCFAs prevents placental growth restriction and vascular insufficiency. These findings highlight the importance of host-microbial symbioses during pregnancy and reveal that the maternal gut microbiome promotes placental growth and vascularization in mice.

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Section: Methodsmentioning

confidence: 99%

The maternal microbiome promotes placental development in mice

Pronovost,

Yu,

Coley-O’Rourke

et al. 2023

Sci. Adv.

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“…Pregnant C57BL/6 mice were intra-amniotically injected with 9 ng of HMGB1/100 µL of PBS (or 100 µL 1x PBS as control) in each amniotic sac on 14.5 dpc and maternal-fetal obstetrical parameters were evaluated by ultrasonography (VisualSonics Inc.) 96 h after, as previously described (68,(76)(77)(78)(79)(80). The fetal heart rate and the peak systolic velocity (PSV) of the umbilical arteries of three to four fetuses from each dam were examined.…”

Section: Evaluation Of Maternal-fetal Obstetrical Parameters By Ultra...mentioning

confidence: 99%

Homeostatic macrophages prevent preterm birth and improve neonatal outcomes by mitigatingin uterosterile inflammation

Garcia‐Flores

Liu

Romero

et al. 2023

Preprint

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Preterm birth (PTB), commonly preceded by preterm labor, is the leading cause of neonatal morbidity and mortality worldwide. Recent advances in next-generation sequencing have revealed that most cases of preterm labor are associated with sterile intra-amniotic inflammation (SIAI), an inflammatory condition without detectable microorganisms. To date, no successful strategies to treat SIAI have been developed. Herein, we present mechanistic proof that treatment with M2-polarized macrophages (M2 Mϕ) can effectively prevent SIAI-induced PTB and neonatal mortality. M2 Mϕ halt the premature pathway of labor by infiltrating maternal and fetal compartments, where they inhibit NLRP3 inflammasome activation triggered by HMGB1. Furthermore, M2 Mϕ dampen the HMGB1-induced inflammatory response in the amniotic cavity and fetal lung. Notably, neonates exposed to HMGB1in uterodisplay a reduced capacity to clear bacterial infection and gut microbiome dysbiosis, which are restored by M2 Mϕ treatment. Our findings provide cogent evidence that M2 Mϕ can serve as a cellular strategy to mitigate PTB and decrease neonatal mortality.

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