High frequency ultrasound characterization of the coagulation process of whole blood

Ossant, F.; Libgot, R.; Coupé, Pierrick; Lermusiaux, Patrick; Patat, F.

doi:10.1109/ultsym.2004.1417869

Cited by 9 publications

(22 citation statements)

References 4 publications

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“…The figure 4 shows the evolution of the velocity during the sedimentation of untreated rat blood and of human blood, as previously reported [1]. Kinetics of variations in velocity for non-clotting human blood are faster and higher than those for non-clotting blood from untreated rats.…”

Section: ) During Red Blood Cell Sedimentationsupporting

confidence: 58%

“…The structures are getting organized. During the first minutes, red blood cells are aggregating, as previously reported [1] [2] [5] [6].…”

Section: Resultsmentioning

confidence: 61%

“…Comparison between human and rat blood 1) During the coagulation process: In previous studies [1] [2], we presented results on the simultaneous measurement, using ultrasonic backscattering and double-transmission modes, to monitor the coagulation process of human blood.…”

Section: Resultsmentioning

confidence: 99%

“…Blood samples were collected into 3.5 ml vacuum citrated tubes (3.2 %) and heated at 37 • C. The coagulation process was initiated by adding calcium chloride (100 µl at 0.5 M concentration) and acoustic measurements were recorded into a blood chamber within 30 min after sampling, in an incubator adjusted at 37 ± 0.1 • C, as previously described ( [1], [2]). …”

Section: ) Rat Blood Collectionmentioning

confidence: 99%

“…Velocity of longitudinal waves was only investigated in this study; this parameter could be considered as a good indicator of the liquid to gel transition of the blood, as described in previous studies [1] [2].…”

Section: Introductionmentioning

confidence: 99%

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1G-3 HF Ultrasound Device Designed to Describe the Whole Blood Coagulation Process: Application to Rats under Anticoagulant Therapy

Libgot

Ossant

Desbuards³

et al. 2006

2006 IEEE Ultrasonics Symposium

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In previous studies [1] [2], we presented a device capable to simultaneously estimate, during the blood clotting process, HF ultrasound parameters both in double-transmission mode (DT-mode) and in backscattering mode (BS-mode). The integrated BS coefficient (IBC) and the effective scatterer size (ESS) were evaluated between 10 and 30 MHz. In DT-mode, sound velocity was estimated with a 45 MHz transducer.The aim of the present work was to confirm several specific results with human whole blood and to validate the method with an animal model: the rat under anticoagulant therapy. Acoustic velocity was estimated during ex-vivo coagulation process of blood from heparin-treated rats.

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Section: ) During Red Blood Cell Sedimentationsupporting

confidence: 58%

“…The structures are getting organized. During the first minutes, red blood cells are aggregating, as previously reported [1] [2] [5] [6].…”

Section: Resultsmentioning

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Section: ) Rat Blood Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

1G-3 HF Ultrasound Device Designed to Describe the Whole Blood Coagulation Process: Application to Rats under Anticoagulant Therapy

Libgot

Ossant

Desbuards³

et al. 2006

2006 IEEE Ultrasonics Symposium

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Measurement of the ultrasonic attenuation coefficient of human blood plasma during clotting in the frequency range of 8 to 22 MHz

Calor-Filho

Machado

2006

Ultrasound in Medicine & Biology

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Micro-ultrasonic Assessment of Early Stage Clot Formation and Whole Blood Coagulation Using an All-Optical Ultrasound Transducer and Adaptive Signal Processing Algorithm

et al. 2022

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Abnormal formation of solid thrombus inside a blood vessel can cause thrombotic morbidity and mortality. This necessitates early stage diagnosis, which requires quantitative assessment with a small volume, for effective therapy with low risk to unwanted development of various diseases. We propose a micro-ultrasonic diagnosis using an all-optical ultrasound-based spectral sensing (AOUSS) technique for sensitive and quantitative characterization of early stage and whole blood coagulation. The AOUSS technique detects and analyzes minute viscoelastic variations of blood at a micro-ultrasonic spot (<100 μm) defined by laser-generated focused ultrasound (LGFU). This utilizes (1) a uniquely designed optical transducer configuration for frequency-spectral matching and wideband operation (6 dB widths: 7–32 MHz and d.c. ∼ 46 MHz, respectively) and (2) an empirical mode decomposition (EMD)-based signal process particularly adapted to nonstationary LGFU signals backscattered from the spot. An EMD-derived spectral analysis enables one to assess viscoelastic variations during the initiation of fibrin formation, which occurs at a very early stage of blood coagulation (1 min) with high sensitivity (frequency transition per storage modulus increment = 8.81 MHz/MPa). Our results exhibit strong agreement with those obtained by conventional rheometry (Pearson’s R > 0.95), which are also confirmed by optical microscopy. The micro-ultrasonic and high-sensitivity detection of AOUSS poses a potential clinical significance, serving as a screening modality to diagnose early stage clot formation (e.g., as an indicator for hypercoagulation of blood) and stages of blood-to-clot transition to check a potential risk for development into thrombotic diseases.

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High frequency ultrasound characterization of the coagulation process of whole blood

Cited by 9 publications

References 4 publications

1G-3 HF Ultrasound Device Designed to Describe the Whole Blood Coagulation Process: Application to Rats under Anticoagulant Therapy

1G-3 HF Ultrasound Device Designed to Describe the Whole Blood Coagulation Process: Application to Rats under Anticoagulant Therapy

Measurement of the ultrasonic attenuation coefficient of human blood plasma during clotting in the frequency range of 8 to 22 MHz

Micro-ultrasonic Assessment of Early Stage Clot Formation and Whole Blood Coagulation Using an All-Optical Ultrasound Transducer and Adaptive Signal Processing Algorithm

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