High-frequency transformation ofLactobacillus casei with plasmid pHY300PLK by electroporation

A practical random mutagenesis system for probiotic Lactobacillus casei using Tn 5 transposition complexes

“…1994). The highest efficiency of L. casei ATCC 27139 was obtained at the pulse voltage of 8·75 kV cm −1 and the resistance of 400 Ω, which were significantly different from those reported previously about several species such as L. casei (Watanabe et al. 1994) or L. fermentum (Wei et al.…”

“…2005), L. plantarum (5·8 × 10 5 CFU μg −1 DNA) (Alegre et al. 2004) and L. casei (1·4 × 10 7 CFU μg −1 DNA) (Watanabe et al. 1994).…”

“…1990). In addition to growth‐phase, concentration of electro‐competent cells has also been reported to affect the transformation efficiency in L. casei (Watanabe et al. 1994).…”

Transformation ofLeuconostoc oenos by electroporation

“…Similar results were obtained for Lactobacillussake, L. curvatus (Gaier et al, 1990) and L. casei (Watanabe et al, 1994). Higher transformation efficiencies were reported for Lactobacillus helveticus subsp, jugurti (Hashiba et al, 1990) and L. lactis subsp, lactis (Mclntyre and Harlander, 1989) when cells from the stationary growth phase were electroporated.…”

An alternative genome-integrated method for undomesticated <i>Bacillus subtilis</i> and related species

“…brevis, B. nematocida, B. thuringiensis, B. antracis, and B. pumilus (Cai et al, 2016;Chen et al, 2003;Ishiwa and Shibahara, 1985;Lertcanawanichakul et al, 2004;Makino et al, 2002;Niu et al, 2015;Oda et al, 2012;Shaw et al, 2000;Toth et al, 2016). In addition, this vector has been successfully used in other related Bacillus such as Lactobacillus, Streptococcus, Paenibacillus, Staphylococcus, and Listeria (Fukuda et al, 2009;Hanawa et al, 2002;Kimoto and Taketo, 2003;Shiratsuchi et al, 2010;Watanabe et al, 1994). It has broad range thermo-stability as it was reported in a psychrophilic Listeria monocytogenes (Okada et al, 2006) as well as a thermophilic B. licheniformis under the selective conditions (Niu et al, 2009;Qiu et al, 2016).…”