High Frequency of Mutations in the<i>PIK3CA</i>Gene Helical and Kinase Coding Regions in a Group of Iranian Patients with High-Grade Glioblastomas: Five Novel Mutations

Derakhshandeh-Peykar, Pupak; Alivi, J; Hossein–Nezhad, Arash; Rautenstrauß, Bernd; Re, Vesal; Doriani, A

doi:10.3109/01677063.2011.623202

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…Eighty six up regulated proteins were enriched in progesterone-mediated oocyte maturation, which is an important process in cell cycle regulation, as its dysregulation can lead to uncontrolled proliferation of cancer cells 65 . In addition, studies have shown that progesterone-mediated oocyte maturation is related to the pathogenesis of glioma 66 .…”

Section: Discussionmentioning

confidence: 99%

Stoichiogenomics reveal oxygen usage bias, key proteins and pathways associated with stomach cancer

Zuo

Zhu

et al. 2019

Sci Rep

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Stomach cancer involves hypoxia-specific microenvironments. Stoichiogenomics explores environmental resource limitation on biological macromolecules in terms of element usages. However, the patterns of oxygen usage by proteins and the ways that proteins adapt to a cancer hypoxia microenvironment are still unknown. Here we compared the oxygen and carbon contents ([C]) between proteomes of stomach cancer (hypoxia) and two stomach glandular cells (normal). Key proteins, genome locations, pathways, and functional dissection associated with stomach cancer were also studied. An association of oxygen content ([O]) and protein expression level was revealed in stomach cancer and stomach glandular cells. For differentially expressed proteins (DEPs), oxygen contents in the up regulated proteins were3.2%higherthan that in the down regulated proteins in stomach cancer. A total of 1,062 DEPs were identified; interestingly none of these proteins were coded on Y chromosome. The up regulated proteins were significantly enriched in pathways including regulation of actin cytoskeleton, cardiac muscle contraction, pathway of progesterone-mediated oocyte maturation, etc. Functional dissection of the up regulated proteins with high oxygen contents showed that most of them were cytoskeleton, cytoskeleton associated proteins, cyclins and signaling proteins in cell cycle progression. Element signature of resource limitation could not be detected in stomach cancer for oxygen, just as what happened in plants and microbes. Unsaved use of oxygen by the highly expressed proteins was adapted to the rapid growth and fast division of the stomach cancer cells. In addition, oxygen usage bias, key proteins and pathways identified in this paper laid a foundation for application of stoichiogenomics in precision medicine.

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Section: Discussionmentioning

confidence: 99%

Stoichiogenomics reveal oxygen usage bias, key proteins and pathways associated with stomach cancer

Zuo

Zhu

et al. 2019

Sci Rep

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“…Annibali et al, (2014) found that Myc inhibition reduces proliferation, increases apoptosis, and, remarkably, elicits the formation of multinucleated cells that then arrest or die by mitotic catastrophe, revealing a new role for Myc in the proficient division of glioma cells. Some results confirmed that PIK3CA mutations occurred in a significant number of human glioblastomas, making it a promising target for therapy, particularly for primary glioblastomas (Weber et al, 2011;Derakhshandeh-Peykar et al, 2012). A meta-analysis by Xiao et al (2012) provided direct and strong evidences that mutations in the PTEN gene were correlated with the poor prognosis of glioma patients (Han et al, 2014).…”

Section: Discussionmentioning

confidence: 61%

Analysis of the gene-protein interaction network in glioma

Zhou

Wj²,

Zhuang

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Glioma is the most aggressive type of brain tumor. Great progress has been achieved in glioma treatment, but the protein-protein interaction networks underlining glioma are poorly understood. We identified the protein-protein interaction network for glioma based on gene expression and predicted biological pathways underlying the molecular complexes in the network. Genes involved in glioma were selected from the Online Mendelian Inheritance in Man (OMIM) database. A literature search was performed using the Agilent Literature Search plugin, and Cytoscape was used to establish a protein-protein interaction network. The molecular complexes in the network were detected using the Clusterviz plugin, and pathway enrichment of molecular complexes was performed using DAVID online. There were 378 glioma genes in the OMIM database. The protein-protein interaction network in glioma contained 1814 nodes, 6471 edges, and 8 molecular complexes. There were 17 pathways (false discovery rate <1), which were related to cytokinecytokine receptor interaction, Toll-like receptor signaling pathway, chemokine signaling pathway, oocyte meiosis, progesterone-mediated oocyte maturation, transmembrane transport of small molecules, metabolism of amino acids, and notch signaling pathway, among others. Our results provide a bioinformatic 14197 Gene-protein interaction network analysis in glioma ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 14196-14206 (2015) foundation for further studies of the mechanisms of glioma.

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“…Mutation p.M1043I was described in previous studies as oncogenic [9]. Mutation p.N1044D has been detected only in a few cases of large intestine carcinoma, endometrial carcinoma and glioblastoma so far [34][35][36]. Using in silico tool PROVEAN, we evaluated its effect on the protein function as neutral (PROVEAN score -0.458).…”

Section: Discussionmentioning

confidence: 99%

Detection of oncogenic mutations in cervical carcinoma using method High Resolution Melting (HRM)

Wayhelova¹,

Mikulášová²,

Smetana³

et al. 2016

neo

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Oncogenic mutations in proto-oncogenes and tumor suppressor genes represent one of key events in cancerogenesis. In this study, we analysed mutation status in PIK3CA, KRAS and EGFR proto-oncogenes and TP53 tumor suppressor gene in a cohort of twenty-four patients diagnosed with squamous cell carcinoma or adenocarcinoma using the screening method "High Resolution Melting" (HRM). Positive findings were confirmed and identified by Sanger sequencing. Totally, we detected DNA sequence changes in targeted regions in seven patients (7/24, 29.2%). In PIK3CA gene, we found six sequence changes in four patients (4/24, 16.7%) and four of them were confirmed as oncogenic mutations. In KRAS gene, we detected sequence changes in four patients (4/24, 16.7%). Conversely, we identified pathogenic or potentially pathogenic sequence changes neither in EGFR nor TP53 genes. Our results suggest that sequence changes are specific neither for a certain histological subtype, clinical stage nor lymph node involvement and they appear independently on the presence of HPV (human papillomavirus) infection since early clinical stages. We observed the correlation between the presence of DNA sequence changes and hTERC gene amplification, but we did not find a significant relationship between the identified DNA sequence changes and detected copy-number alterations using the technique of array-CGH (array-based comparative genomic hybridization). Regardless our results confirmed an important role of oncogenic mutations in PIK3CA and KRAS genes in the neoplastic transformation process in the cervical carcinoma pathogenesis. Their identification in the early clinical stages should encourage further studies to better understand these mutations and exploit them for more detailed diagnostics.

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High Frequency of Mutations in thePIK3CAGene Helical and Kinase Coding Regions in a Group of Iranian Patients with High-Grade Glioblastomas: Five Novel Mutations

Cited by 5 publications

References 17 publications

Stoichiogenomics reveal oxygen usage bias, key proteins and pathways associated with stomach cancer

Stoichiogenomics reveal oxygen usage bias, key proteins and pathways associated with stomach cancer

Analysis of the gene-protein interaction network in glioma

Detection of oncogenic mutations in cervical carcinoma using method High Resolution Melting (HRM)

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