High Frequency of Drug Resistance Mutations in Human Immunodeficiency Virus Type 1-Infected Korean Patients Treated with HAART

Sung, Heungsup; Jung, Yoojin; Kang, Moon Won; Bae, In-Gyu; Chang, Hyun‐Ha; Woo, Jun Hee; Cho, Young K.

doi:10.1089/aid.2007.0008

Cited by 19 publications

(18 citation statements)

References 15 publications

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“…Denatured DNA samples (10 l) were amplified by nested PCR. Details are described in earlier reports (29)(30)(31). The outer and inner primer pairs employed were HXB2/half RT and PO1/RIT137, The amplified products were purified and sequenced directly using the primers PO1 and RIT137 or 527.…”

Section: Methodsmentioning

confidence: 99%

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Beneficial Effects of a Combination of Korean Red Ginseng and Highly Active Antiretroviral Therapy in Human Immunodeficiency Virus Type 1-Infected Patients

Sung

Jung²,

Cho³

2009

Clin Vaccine Immunol

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To determine whether Korean red ginseng (KRG) has beneficial effects on human immunodeficiency virus type 1 (HIV-1)-infected patients administered highly active antiretroviral therapy (HAART), we analyzed the CD4 T-cell count, viral load, and resistance mutations to HAART in 46 individuals. Thirteen patients harbored resistance mutations at baseline. The study population was divided into two groups: specifically, a group treated with a combination of HAART plus KRG (23 patients) and a group treated with HAART alone (23 patients). The annual increase in CD4 T-cell count in the combination group was significantly higher than that in the group treated with HAART alone (P < 0.05). Overall, 21 patients harbored resistance mutations after 3 years of therapy. Following exclusion of 13 patients displaying baseline resistance mutations, 7.1% of patients (1/14) in the combination group and 42.1% (8/19) in the HAART group were identified with resistance mutations. One patient with baseline resistance mutations in the combination group did not display resistance mutations 3 years after HAART therapy. High-level resistance mutations were significantly lower in the combination group than in the group treated with HAART alone. Five patients showed no improvement in viral copy number (26.3% [5/19]) in the combination group and 9 (45.0% [9/20]) showed no improvement in the HAART-only group. Our data support the clinical utility of KRG intake during HAART therapy.

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Section: Methodsmentioning

confidence: 99%

“…Drug resistance mutations were determined on the basis of published guidelines (19). The details are provided in an earlier report (31).…”

Section: Methodsmentioning

confidence: 99%

Beneficial Effects of a Combination of Korean Red Ginseng and Highly Active Antiretroviral Therapy in Human Immunodeficiency Virus Type 1-Infected Patients

Sung

Jung²,

Cho³

2009

Clin Vaccine Immunol

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“…Three HPs (nos. [21][22][23] were infected with clotting factor purchased outside Korea before 1987 (Table 1). Except for a dead patient diagnosed in 1988 (case 4), 19 we included all homosexual patients diagnosed before 1990 (n ¼ 15) and 17 of 18 homosexual patients diagnosed in 1990.…”

Section: Twenty Hiv-1-infected Hps and Local Controlsmentioning

confidence: 99%

“…Details are described in earlier reports. 18,[20][21][22] The subsequent amplicons were directly sequenced using Applied Biosystems 3730XL (Macrogen Inc., Seoul, Korea).…”

Section: Rna Preparation and Pol Gene Amplificationmentioning

confidence: 99%

Phylogenetic Analysis of Full-LengthpolGene from Korean Hemophiliacs and Plasma Donors Infected with Korean Subclade B of HIV Type 1

Cho

Jung

Foley

2011

AIDS Research and Human Retroviruses

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There was an outbreak of HIV-1 transmission among 20 out of 122 Korean hemophiliacs from 1990. We assessed the genetic relationships among HIV-1 viruses found in three cash-paid plasma donors whose preseroconversion plasma was used to produce Korean-made clotting factor, 20 hemophiliacs infected with HIV-1 in Korea, three hemophiliacs infected with HIV-1 from clotting factor manufactured outside Korea, and 71 local control patients infected with the Korean subclade of HIV-1 subtype B (KSB). Full-length pol gene sequences (2841 bp) of viruses from frozen stored serum, samples obtained 1-3 years after diagnosis, were amplified by RT-PCR and sequenced by direct DNA sequencing. Phylogenetic and signature pattern analyses were used to investigate the relationships among the sequences. Donors O and P were associated with two clusters, of 8 and 12 hemophiliacs, respectively, which were demarcated from the 71 KSB-infected local control patients and donor R. These data confirm that HIV-1 transmission to 20 hemophiliacs occurred through infusion of Korean-made clotting factor.

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“…These drugs target different steps of HIV-1 life cycle. Despite the significant effect of the current HIV therapy method, there are many disadvantages associated with the market drugs, including drug-related side effects [16], inconvenient dosing requirements and drug resistance [17][18][19]. Therefore, identification and development of new HIV-1 inhibitor is still an urgent task for anti-HIV-1 drug research nowadays.…”

Section: Hiv-1 High-throughput Screening Inhibitor Virus-cell-basementioning

confidence: 99%

Establishment of a high-throughput screening system for universal anti-HIV targets

et al. 2010

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The process of identifying novel human immunodeficiency virus 1 (HIV-1) inhibitors presents a challenge for industrial and scientific research. Virus-cell-based screening approaches offer some advantages in the quest for novel inhibitors because they include multiple targets in a single screen and in some cases reveal targets not captured in biochemical assays. In this study, a high-throughput screening (HTS) system for HIV-1 inhibitors was developed, which allows the simultaneous screening of all the HIV-1 targets required for replication in the cell culture. HeLa/cluster of differentiation 4 (CD4)/long terminal repeat (LTR) indicator cells, which stably expressed high levels of HIV receptor CD4 and contained the firefly luciferase reporter gene under the control of the HIV-1 LTR promoter, were generated. The expression of CD4 and LTR function in this cell line was validated by Western blot and luciferase assay. MT2 cells, a human T-cell leukemia cell line that support high levels of HIV-1 replication, were infected with HIV-1, and then the infected MT2 cells were co-cultured with HeLa/CD4/LTR cells. In the optimized assay conditions, HIV-1 replication occurs rapidly in the MT2 cells, resulting in the infection of the HeLa/CD4/LTR cells and a significant induction of luciferase signals through LTR, which is activated by the expression of HIV-1 tat gene. The luciferase signals of HeLa/CD4/LTR cells co-cultured with HIV-1 infected MT2 cells were significantly stronger than the signals of noninfected HeLa/CD4/LTR cells (P < 0.001). The inhibitory effects of HIV-1 inhibitors (3′-Azido-3′-deoxythymidine [AZT], efavirenz [EFV], and nevirapine [NVP]) were evaluated with this assay, and the inhibitory concentration 50% (IC 50 ) values of the above three inhibitors were 58, 1.4, and 85 nmol/L, respectively, indicating that the assay provides the necessary sensitivity for identifying antiviral molecules. The Z' factor had a value of 0.563, indicating this is a very robust assay. These results suggested that HIV-1 infection assay represents a novel approach to HIV-1 antiviral screening that allows for the effective execution of HTS campaigns. HIV-1, high-throughput screening, inhibitor, virus-cell-based assayCitation: Yin Q, Zhuang D M, Jiang Y Q, et al. Establishment of a high-throughput screening system for universal anti-HIV targets. Chinese Sci Bull, 2010, 55: 937−942,

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High Frequency of Drug Resistance Mutations in Human Immunodeficiency Virus Type 1-Infected Korean Patients Treated with HAART

Cited by 19 publications

References 15 publications

Beneficial Effects of a Combination of Korean Red Ginseng and Highly Active Antiretroviral Therapy in Human Immunodeficiency Virus Type 1-Infected Patients

Beneficial Effects of a Combination of Korean Red Ginseng and Highly Active Antiretroviral Therapy in Human Immunodeficiency Virus Type 1-Infected Patients

Phylogenetic Analysis of Full-LengthpolGene from Korean Hemophiliacs and Plasma Donors Infected with Korean Subclade B of HIV Type 1

Establishment of a high-throughput screening system for universal anti-HIV targets

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