High Frequency of Doubled Haploid Plant Production in Spelt Wheat

Lantos, Csaba; Jenes, Barnabás; Bóna, L.; Cserháti, Mátyás; Pauk, János

doi:10.1515/abcsb-2016-0014

Cited by 9 publications

(7 citation statements)

References 24 publications

(34 reference statements)

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“…In contrast to the efficiency of the present report, Lantos et al, [30] reported an efficient anther culture protocol for spelt wheat. The number of ELS was produced 62.2 per 100 anthers.…”

Section: Discussioncontrasting

confidence: 86%

Inductive Effects of Carbohydrates and Plant Regeneration via Anther Culture in Madagascar Periwinkle

Narkhedkar¹,

Tidke²

2021

azb

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Anther culture has emerged as an alternative for traditional plant breeding practices for homozygosity development, which in the case of medical plants resulting in increased yield of phytoconstituents. The response of anthers during culture is governed by the various constituents of the nutrient media. The carbohydrate in the nutrient media plays a pivotal role as it acts as a source of carbon skeleton and maintains the osmoticum. In the present research, anthers of Catharanthus roseus (L.) G. Don. at early to late uninucleate stage of development were selected for the experiment. The anthers were cultured to evaluate the androgenic potential under the influence of different types and concentrations of carbohydrates viz. sucrose, glucose, maltose, and galactose. It was found that out of four carbohydrates except maltose, all performed best at 30 gm/l. The maximum rate of androgenic callus induction was noted in sucrose at 30 gm/l (69.66±1.52%). All the carbohydrates showed the least rate of calli induction at high concentration i.e. 70 gm/l. The embryo like structures were able to develop on the media supplemented with the combination of 1 mg/l BAP, 0.5 mg/l Kinetin and 0.5 mg/l NAA (13.33±5.77%). The ploidy analysis of randomly selected 30 calli and 10 leaves revealed that Catharanthus roseus has haploid regeneration tendencies and also possesses a tendency for spontaneous double haploid formation.

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“…In contrast to the efficiency of the present report, Lantos et al, [30] reported an efficient anther culture protocol for spelt wheat. The number of ELS was produced 62.2 per 100 anthers.…”

Section: Discussioncontrasting

confidence: 86%

Inductive Effects of Carbohydrates and Plant Regeneration via Anther Culture in Madagascar Periwinkle

Narkhedkar¹,

Tidke²

2021

azb

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“…Furthermore, ten breeding targeted F 1 genotypes were involved in our research and breeding program. In F 1 generation of twenty-two spelt wheat combinations, in vitro anther culture method was tested to produce genetically pure lines for spelt breeding program [22,23,29].…”

Section: Discussionmentioning

confidence: 99%

“…Well-established DH plant production methods are applicable for breeding and research of some crop plants, for example: Barley, wheat, triticale, maize, and rice [12,13,14,15]. In spite of these benefits, only a few published data can be found in connection with DH plant production of spelt wheat [20,21,22,23,24].…”

Section: Introductionmentioning

confidence: 99%

Utilization of in Vitro Anther Culture in Spelt Wheat Breeding

Lantos

Purgel

Ács

et al. 2019

Plants

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The efficiency of in vitro anther culture was screened in a full diallel population of four spelt wheat genotypes and ten F1 hybrids. Genotype dependency was observed based on the data of embryo-like structures (ELS), green-, albino plantlets. In the diallel population and ten F1 hybrids, the green plantlets production ranged from 13.75 to 85.00 and from 6.30 to 51.00, respectively. The anther culture-derived plants of F1 hybrids were grown up in the nursery. At the harvest, 436 spontaneous doubled haploid (DH) plants were identified among the 1535 anther culture-derived transplanted and grown up individual plants. The mean of spontaneous rediploidization was 28.4% which ranged from 9.76% to 54.24%. In two consecutive years, the agronomic values of ‘Tonkoly.pop1’ advanced line were compared with seven DH lines of ‘Tonkoly.pop1’ in the nursery. The DH lines achieved competitive values in comparison with ‘Tonkoly.pop1’ advanced line based on the 11 measured parameters (heading date, plant height, yield, hardness, width and length of seed, TKW, hulling yield, flour yield, protein and wet gluten content). These observations presage the efficient utilization of anther culture in spelt wheat breeding.

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“…The compositions of plant regeneration medium influence the efficiency of green plantlets production in AC of rice. The MS basal media have been frequently applied for plant regeneration in rice AC [8,11,12,38]. Sucrose is a general carbon source during plant regeneration, while a wide range of growth regulators (BAP, IAA, NAA, kinetin and zeatin), and their combinations were applied for plant regeneration of AC-derived rice calli [11,12,21,23,24,26,28,38].…”

Section: Effect Of Exogenous Growth Regulators On the Regeneration Of...mentioning

confidence: 99%

Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

Lantos

Jancsó

Székely

et al. 2023

Plants

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Anther culture is an efficient biotechnological tool in modern plant breeding programs to produce new varieties and parental lines in hybrid seed productions. However, some bottlenecks—low induction rate, genotype dependency, albinism—restrict the widespread utilization of in vitro anther culture in rice breeding, especially in Oryza sativa ssp. indica (indica) genotypes, while an improved efficient protocol can shorten the process of breeding. Three different induction media (N6NDK, N6NDZ, Ali-1) and four plant regeneration media (mMSNBK1, MSNBK3, MSNBKZ1, MSNBKZ2) were tested with five indica rice genotypes to increase the efficiency of in vitro androgenesis (number of calli and regenerated green plantlets). The production of calli was more efficient on the N6NDK medium with an average 88.26 calli/100 anthers and N6NDZ medium with an average of 103.88 calli/100 anthers as compared to Ali-1 with an average of 6.96 calli/100 anthers. The production of green plantlets was greater when calli was produced on N6NDK medium (2.15 green plantlets/100 anthers) compared when produced on to N6NDZ medium (1.18 green plantlets/100 anthers). Highest green plantlets production (4.7 green plantlets/100 anthers) was achieved when mMSNBK1 plant regeneration medium was used on calli produced utilizing N6NDK induction medium. In the best overall treatment (N6NDK induction medium and mMSNBK1 plant regeneration medium), four tested genotypes produced green plantlets. However, the genotype influenced the efficiency, and the green plantlets production ranged from 0.4 green plantlets/100 anthers to 8.4 green plantlets/100 anthers. The ploidy level of 106 acclimatized indica rice plantlets were characterized with flow cytometric analyses to calculate the percentage of spontaneous chromosome doubling. Altogether, 48 haploid-, 55 diploid-, 2 tetraploid- and 1 mixoploid plantlets were identified among the regenerant plantlets, and the spontaneous chromosome doubling percentage was 51.89%. Utilization of DH plants have been integrated as a routine method in the Hungarian rice breeding program. The tetraploid lines can be explored for their potential to offer new scopes for rice research and breeding directions in the future.

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High Frequency of Doubled Haploid Plant Production in Spelt Wheat

Cited by 9 publications

References 24 publications

Inductive Effects of Carbohydrates and Plant Regeneration via Anther Culture in Madagascar Periwinkle

Inductive Effects of Carbohydrates and Plant Regeneration via Anther Culture in Madagascar Periwinkle

Utilization of in Vitro Anther Culture in Spelt Wheat Breeding

Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

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