High frequency of cytomixis observed at zygotene in tetraploid Lippia alba

Reis, Aryane Campos; Sousa, Saulo Marçal de; Viccini, Lyderson Facio

doi:10.1007/s00606-015-1249-3

Cited by 29 publications

(26 citation statements)

References 51 publications

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“…The wounding of the tissues during the excision of explant can trigger the damaging oxidative burst [73]. Along with other factors that induce somaclonal variation, the natural instability of L. alba genome already reported [28,36,38], might compose a complex scenario responsible for genetic variation detected in L. alba.…”

Section: Discussionmentioning

confidence: 98%

“…Reis, et al [28] reported that there is a considerable variation in genome size of L. alba. Five different chromosome numbers (2n = 30, 38,45,60,90) and different DNA amounts were detected in 106 accessions. Another aspect is the natural karyotype variation observed even within the diploids.…”

Section: Discussionmentioning

confidence: 99%

“…[25,30,31,32,33,34]. Few studies have been done about biological aspects of the species [28,35,36,37,38]. Five chromosome numbers were reported [28] raising the importance to investigate the genetic variation of the species using different approaches.…”

Section: Flow Cytometry and Molecular Markers Havementioning

confidence: 99%

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Genome Status of Lippia alba Polyploid Complex Long-term in vitro Cultivated

Julião

Lopes

Zorzatto

et al. 2020

EJMP

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This study is the first report of a genetic stability analysis of a polyploid complex maintained in vitro for a long-time. Twenty-two accessions of Lippia alba, a medicinal species of economic importance, had been maintained under in vitro culture conditions for 7 years through sprouting of axillary buds. Four clones of each accession were analyzed, being three plants from in vitro bank and one cultivated in the field. We investigated the genetic stability of diploid, aneuploid, triploid, tetraploid, and hexaploid accessions. The investigation was carried out using flow cytometry, inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers. No significant variation in nuclear DNA content was observed between the in vitro conserved plants and their respective field plant. Out of 23 ISSR primers screened, 8 primers were found to produce clear reproducible bands resulting in a total of 5456 bands. 86.36% of the analyzed plantlets (19 accessions) showed at least one polymorphic band. The polymorphic rate ranged from 1.61 to 33.87%. The SSR markers were used to confirm the absence or low occurrence of variation in accessions that showed no polymorphism or polymorphism for only one ISSR primer. The genetic instability detected in our study at the molecular level may be attributed to the natural instability of L. alba genome combined with the long-time in vitro maintenance.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Genome Status of Lippia alba Polyploid Complex Long-term in vitro Cultivated

Julião

Lopes

Zorzatto

et al. 2020

EJMP

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“…Some scientists suggest that the chromosomal polymorphisms of microsporocytes and pollen grains, as well as a large variety of natural polyploid cytotypes in many plant species confirm the involvement of migrant chromatin in the karyotype restructuring (Zheng et al, 1987;Zhou, 2003;Malallah and Attia, 2003;Lattoo et al, 2006;Ghaffari, 2006;Lattoo et al. 2006;Negron-Ortiz, 2007;Kumar and Singhal, 2008;Pierre e de and Sousa, 2011;Singhal et al, 2011a,b;Rana et al, 2013;Mandal et al, 2013;Mursalimov et al, 2013Mursalimov et al, , 2015bReis et al, 2015).…”

Section: Peculiarities Of Nuclear Interactions and Nuclear Translocationmentioning

confidence: 99%

“…(Tarkowska, ; Bobak and Herich, ; Narain, ; Bauchan et al, ; Dwivedi et al, ; Bedi, ; Grodzinsky et al, ; de Souza and Pagliarini, ; Ghaffari, ; Sidorchuk et al, ; Kravets, , ; Negron‐Ortiz, ). Cytomixis rather frequently occurs in polyploids and within the same species in the forms with different ploidy levels (Falistocco et al, ; Bellucci et al, ; Ghaffari, ; Lattoo et al, ; Kumar and Singhal, , ; Singhal et al, ; Guan et al, ; Mursalimov et al, ; Reis et al, ). Climatic and seasonal factors, especially extreme temperatures and drought also increase cytomixis activity (Soman and Bhavanandan, ; Kumar and Singhal, , ; Zinn et al, ; Pecrix et al, ; Singhal et al, ; Kravets et al, ; Malik and Gupta, ; de Storme and Geelen, ; de Storme and Mason, ).…”

Section: Introductionmentioning

confidence: 99%

Cytoskeleton and nucleoskeleton involvement in processes of cytomixis in plants

Kravets

Yemets

Blume

2017

Cell Biology International

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Cytomixis is a form of cell-to-cell nuclear migration that involves the interaction of dynamic cytoskeletal components with the nucleus through signalling systems and linker complexes. In cytomixis two known mechanisms can be involved: actomyosin and/or microtubules and their associated motors. Perinuclear actin anchors and determines the direction of nuclear movement. In microsporogenesis cytomixis is probably initiated by a cascade of signals that trigger prophase reorganization of nucleus and cytoskeleton, and is a result of cytoskeletal protein activation, as well as a weakening of mechanisms responsible for anchoring the nucleus. The interactions between nuclei and the cytoskeleton are mediated by linker complexes that play a major role in nuclear positioning and shape, chromatin-nuclear envelope interactions, nucleoskeleton organization, gene expression and genome organization. Other contributing factors include changes in the protein composition and post-translational modifications that alter protein conformation. Cytomixis appears also to have relevance to higher order structuring, influencing tissue and organ architecture, causing collective forms of cell interactions and information exchange within a single continuum. In this review we summarize our current understanding of the cytoskeleton dynamic function in cytomictic nuclear migration.

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Occurrence of 2nmicrospore production in diploid interspecific hybrids between the wild parental species of peanut (Arachis hypogaeaL., Leguminosae) and its relevance in the genetic origin of the cultigen

et al. 2020

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Unreduced (2n) gametes are the driving force that leads to polyploidization of plants in nature and an important tool for ploidy breeding. Peanut (Arachis hypogaea L., Leguminosae) seems most likely to have originated by sexual polyploidization through interspecific hybridization between the wild diploid relatives—A. duranensis Krapov. & W.C. Greg. and A. ipaënsis Krapov. & W.C. Greg—and subsequent binding of the unreduced gametes in the hybrid. Therefore, aiming to contribute to understanding the event of polyploid origin of this important crop, we made diploid hybrids between A. duranensis and A. ipaënsis and then investigated the production of unreduced gametes in the hybrids by analyzing the size range of pollen, the constitution of the sporads, and the microsporogenesis process. The meiotic abnormalities found, such as persistent bridges, tripolar spindles, and cytomixis processes, would lead to the formation of restitution nuclei in both the first and second meiotic division. These failures in the regularity of meiosis lead to the formation of dyads and triads, and consequently to 2n pollen grains. These findings constitute the first evidence of the formation of unreduced gametes in intergenomic hybrids obtained between the wild progenitors of A. hypogaea and enable us to propose a model of origin of the crop through sexual polyploidization.

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High frequency of cytomixis observed at zygotene in tetraploid Lippia alba

Cited by 29 publications

References 51 publications

Genome Status of Lippia alba Polyploid Complex Long-term in vitro Cultivated

Genome Status of Lippia alba Polyploid Complex Long-term in vitro Cultivated

Cytoskeleton and nucleoskeleton involvement in processes of cytomixis in plants

Occurrence of 2nmicrospore production in diploid interspecific hybrids between the wild parental species of peanut (Arachis hypogaeaL., Leguminosae) and its relevance in the genetic origin of the cultigen

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