High frequency of Bob1 lo T follicular helper cells in florid reactive follicular hyperplasia

Matsumiya, Hiroshi; Kawata, Keiichiro; Kamekura, Ryuta; Tsubomatsu, Chieko; Jitsukawa, Sumito; Asai, Takamasa; Akasaka, Syunsuke; Kamei, Motonari; Yamashita, Keiji; Ito, Fumie; Kubo, Terufumi; Sato, Noriyuki; Takano, Kenichi; Himi, Tetsuo; Ichimiya, Shingo

doi:10.1016/j.imlet.2017.07.012

Cited by 1 publication

(2 citation statements)

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“…Although OSA tonsils contained higher numbers of T FH cells than RT tonsils, OSA tonsil-derived T FH cells were inferior in terms of providing B cell help. Functional impairment of this nature was linked to the reduced expression of the transcription co-activator Bob1 and decreased IL-4 production in T FH cells from OSA tonsils (48). Dan et al published similar results in a study on tonsils from children with RT compared to OSA tonsils, where RT-tonsils showed smaller GC and a lower frequency of GC-T FH cells (49).…”

Section: Discussionmentioning

confidence: 90%

“…We additionally explored potential sources of T cell help to B cells but found no changes in the T cell compartment, including T FH cells, in large versus small tonsils. Matsumiya et al have previously demonstrated that, in adults, tonsils from patients with OSA contain considerably larger GC areas with increased frequency of GC B cells compared to those from patients with recurrent tonsillitis (RT) (48). Although OSA tonsils contained higher numbers of T FH cells than RT tonsils, OSA tonsil-derived T FH cells were inferior in terms of providing B cell help.…”

Section: Discussionmentioning

confidence: 99%

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The Tonsil Lymphocyte Landscape in Pediatric Tonsil Hyperplasia and Obstructive Sleep Apnea

et al. 2021

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Tonsil hyperplasia is the most common cause of pediatric obstructive sleep apnea (OSA). Despite the growing knowledge in tissue immunology of tonsils, the immunopathology driving tonsil hyperplasia and OSA remains unknown. Here we used multi-parametric flow cytometry to analyze the composition and phenotype of tonsillar innate lymphoid cells (ILCs), T cells, and B cells from pediatric patients with OSA, who had previous polysomnography. Unbiased clustering analysis was used to delineate and compare lymphocyte heterogeneity between two patient groups: children with small tonsils and moderate OSA (n = 6) or large tonsils and very severe OSA (n = 13). We detected disturbed ILC and B cell proportions in patients with large tonsils, characterized by an increase in the frequency of naïve CD27-CD21hi B cells and a relative reduction of ILCs. The enrichment of naïve B cells was not commensurate with elevated Ki67 expression, suggesting defective differentiation and/or migration rather than cellular proliferation to be the causative mechanism. Finally, yet importantly, we provide the flow cytometry data to be used as a resource for additional translational studies aimed at investigating the immunological mechanisms of pediatric tonsil hyperplasia and OSA.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%