High-frequency in vitro plantlet regeneration in Lilium davidii var. unicolour Salisb, an important edible and medicinal plant, and confirmation of genetic fidelity of regeneration plantlets using ISSR markers

Lu, Yang; Chao, Liqin; Su, Xiuhong; Wang, Chunyan; Dong, Chengming; Chen, Suiqing

doi:10.1007/s11816-021-00690-5

Cited by 13 publications

(5 citation statements)

References 29 publications

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“…napalense [ 48 ]. In contrast, Yang et al [ 33 ] employed IBA and NAA for in vitro rooting of shoots in Lilium davidii var. unicolour Salisb.…”

Section: Discussionmentioning

confidence: 99%

“…This measure is critical to facilitate the successful proliferation and preservation of rare plant species [ 30 ]. In recent years, Polymerase Chain Reaction (PCR) techniques, specifically Inter-Simple Sequence Repeats (ISSR), have proven to be effective in assessing the genetic fidelity of regenerated plantlets in lilies [ 31 – 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Application of machine learning in in vitro propagation of endemic Lilium akkusianum R. Gämperle

Tütüncü

2024

PLoS ONE

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A successful regeneration protocol was developed for micropropagation of Lilium akkusianum R. Gämperle, an endemic species of Türkiye, from scale explants. The study also aimed to evaluate the effects of Meta-Topolin (mT) and N6-Benzyladenine (BA) on in vitro regeneration. The Murashige and Skoog medium (MS) supplemented with different levels of α-naphthaleneacetic acid (NAA)/BA and NAA/mT were used for culture initiation in the darkness. The highest callus rates were observed on explants cultured on MS medium with 2.0 mg/L NAA + 0.5 mg/L mT (83.31%), and the highest adventitious bud number per explant was 4.98 in MS medium with 0.5 mg/L NAA + 1.5 mg/L mT. Adventitious buds were excised and cultured in 16/8 h photoperiod conditions. The highest average shoot number per explant was 4.0 in MS medium with 2.0 mg/L mT + 1.0 mg/L NAA. Shoots were rooted with the highest rate (90%) in the medium with the 1.0 mg/L IBA, and the highest survival rate (87.5%) was recorded in rooted shoots in the same medium. The ISSR marker system showed that regenerated plantlets were genetically stable. Besides traditional tissue culture techniques used in the current study, the potential for improving the effectiveness of L. akkusianum propagation protocols by incorporating machine learning methodologies was evaluated. ML techniques enhance lily micropropagation by analyzing complex biological processes, merging with traditional methods. This collaborative approach validates current protocols, allowing ongoing improvements. Embracing machine learning in endemic L. akkusianum studies contributes to sustainable plant propagation, promoting conservation and responsible genetic resource utilization in agriculture.

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“…napalense [ 48 ]. In contrast, Yang et al [ 33 ] employed IBA and NAA for in vitro rooting of shoots in Lilium davidii var. unicolour Salisb.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Application of machine learning in in vitro propagation of endemic Lilium akkusianum R. Gämperle

Tütüncü

2024

PLoS ONE

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“…The findings of this study support the maintenance of genetic similarity of direct regenerated plants with the mother plants during the in vitro process. The RAPD and ISSR techniques have been applied widely to examine the genetic homogeneity in various in vitro derived plants, such as Magnolia sirindhorniae (Cui et al, 2019 ), Ranunculus wallichianus (Srinivasan et al, 2021 ), P. africana (Komakech et al, 2020 ), Lillium davidii (Yang et al, 2021 ), and Thunbergia coccinea (Sultana et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Ahmad

Yadav²,

Shahzad³

et al. 2022

Front. Plant Sci.

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Casuarina equisetifolia is an important tree of the forest, cultivated in tropical and subtropical regions, providing fuelwood, land reclamation, dune stabilization, paper production, and nitrogen fixation. We have developed a systematic in vitro propagation protocol in C. equisetifolia using nodal segments (NS). Murashige and Skoog (MS) medium augmented with BA (5.0 μM) and NAA (0.5 μM) gave rise to a maximum of 32.00 ± 0.31 shoots per explant (S/E) with shoot length (SL) of 3.94 ± 0.02 cm, and a maximum of 70% regeneration potential (RP) was recorded after 8 weeks of post inoculation. For root induction, in vitro derived shoots were transferred to the nutrient medium consisting of a half-strength (½) MS medium augmented with 2.5 μM NAA, which produced a maximum of 12.68 ± 0.33 roots/shoot (R/S) with 3.04 ± 0.50 cm root length (RL) in 60% of culture after 6 weeks. Micropropagated plants with healthy shoots and roots were successfully acclimatized in vermicompost + garden soil + sand (1:2:1) and a maximum survival percentage of 95.1% was recorded. NS was taken from a 6-weeks-old in vitro derived plant of C. equisetifolia for synthetic seed production, and it was reported that CaCl2 · 2H2O (100 mM) + Na2-alginate (4%) resulted in clear and uniform beads. Furthermore, the maximum conversion of synthetic seeds into plantlets occurred over a period of 4 weeks of storage at 4°C. Scanning Electron Microscopy (SEM) revealed the formation of direct shoot buds without any intermediate callus formation. In addition, the chlorophyll and carotenoid contents of the direct regenerated and mother plant were compared. Similarly, RAPD and ISSR primers were used for genetic homogeneity assessment of the direct regenerated plants, where a total of 18 and 19, respectively, clear and reproducible bands with 100% monomorphism were recorded. The developed micropropagation protocol can certainly be used for large-scale multiplication and germplasm preservation of C. equisetifolia. It will also help in meeting the growing demands of C. equisetifolia in the forest industry.

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“…Several factors can optimize AR induction, including PGRs type and concentration [13], light [14], temperature [15], and other factors. Nutrient de ciency can also serve as a cue for the initiation and elongation of ARs.…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng

Xiong,

Chen,

Liu

et al. 2024

Preprint

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Background The extract from Metasequoia glyptostroboides Hu et Cheng, a rare and endangered species native to China, exhibits numerous biological and pharmacological activities. The species is recalcitrant to rooting during micropropagation, a challenge that has yet to be resolved. In this study, transcriptomic and hormonal analyses were conducted to appreciate the molecular mechanism of adventitious root (AR) formation in optimized rooting conditions. Results The use of 2/5-strength Woody Plant Medium (WPM) significantly promoted AR formation of M. glyptostroboides shoots while the content of endogenous auxin, cytokinins and gibberellins (GAs) varied at different stages of AR formation. Transcriptomic analysis showed the significant up- or down-regulation of differentially expressed genes (DEGs) associated with plant hormone signal transduction and the phenylpropanoid biosynthesis pathway in response to 2/5-strength WPM. DEGs related to the biosynthesis of indole-3-acetic acid, cytokinins and GAs were identified. Transcript factors involved in 13 families were also revealed. A weighted gene co-expression network analysis indicated a strong correlation between hormones and genes involved in plant hormone signal transduction and the phenylpropanoid biosynthetic pathway. Conclusions These results indicate that the AR-promoting potential of 2/5-strength WPM in M. glyptostroboides was due to complex interactions between hormones and the expression of genes related to plant hormone signal transduction and the phenylpropanoid biosynthetic pathway.

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High-frequency in vitro plantlet regeneration in Lilium davidii var. unicolour Salisb, an important edible and medicinal plant, and confirmation of genetic fidelity of regeneration plantlets using ISSR markers

Cited by 13 publications

References 29 publications

Application of machine learning in in vitro propagation of endemic Lilium akkusianum R. Gämperle

Application of machine learning in in vitro propagation of endemic Lilium akkusianum R. Gämperle

Micropropagation, encapsulation, physiological, and genetic homogeneity assessment in Casuarina equisetifolia

Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng

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