High-Frequency Androgenic Green Plant Regeneration in Indica Rice for Accelerated Breeding

Samantaray, Sanghamitra; Dash, Byomkesh; Bhuyan, Sudhansu Sekhar; Chidambaranathan, Parmeswaran; Katara, Jawahar Lal; Verma, Ram Lakhan; Devanna, B. N.; Chattopadhyay, Krishnendu; Kumar, Awadesh; Anandan, A.

doi:10.1007/978-3-030-41866-3_10

Cited by 3 publications

(1 citation statement)

References 55 publications

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“…In the development of new hybrid rice varieties using cytoplasmic male sterility (CMS) to obtain restorer lines, [6] reported that it needed at least three to four years. To provide rice varieties with better quantity and quality, climate-resilient, as well as resistant to pests and diseases in a short period, plant breeding re-orientation is needed to shorten the breeding cycles [7]. Haploid breeding offers a rapid method to produce new varieties to provide food for two or three decades ahead [8].…”

Section: Introductionmentioning

confidence: 99%

Comparison of three different RNA extraction kits for transcriptome analysis of Indonesian rice (Oryza sativa L) explant for anther culture

Fatimah,

Purwoko,

Rosyid

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Recalcitrancy is the main problem in rice anther culture, especially in an Indica subspecies. It is well known that recalcitrancy is genotype dependent, and the effort to tackle this problem is mostly in the manipulations of media. Uncovering the genes responsible for recalcitrancy will benefit to enhance the success of this technique to accelerate the breeding process of Indonesian rice varieties which mostly belong to the indica subspecies. RNA-Seq is considered one of the best approaches to finding candidate genes responsible for specific traits including recalcitrant in anther culture. The aim of this study was to obtain the high concentration and good quality of RNA extracted methods using various extraction kits. The RNA extraction was carried out using Tripure, Promega, and RibospinTM Plant (Geneall) kit methods. Quality of RNA provided by the Promega kit showed good quality of RNA in all three genotypes tested ranging from 2.06±0.01-2.11±0.00 for A260/280 and 1.86±0.14-1.95±0.10 for A260/230.

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Section: Introductionmentioning

confidence: 99%

Comparison of three different RNA extraction kits for transcriptome analysis of Indonesian rice (Oryza sativa L) explant for anther culture

Fatimah,

Purwoko,

Rosyid

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Panicle Formation Order Affects Rice Oryza sativa L. in vitro Androgenesis

Ilyushko¹,

Romashova²,

Гученко³

2023

Russ. Agricult. Sci.

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Panicle formation order influence in rice Oryza sativa L in vitro androgenesis

Ilyushko,

Romashova,

Guchenko

2023

Rossijskaâ selʹskohozâjstvennaâ nauka

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A comparative analysis of rice Oryza sativa L. main and lateral panicles in androgenesis in vitro on F1 plants of two hybrid combinations: L×3P and K×3P to increase the number of cultivated anthers during the season was carried out. Donor plants were grown on the growing area in metal vessels filled with soil. The main panicle was used from three L×3P hybrids and one K×3P hybrid, single lateral panicle was cut from six L×3P plants and three K×3P plants. The genotypes number was calculated as the product of the calli number with doubled haploids by 1.25. In vitro culture 2050 anthers were introduced. According to the intensity of callus formation, the number of green regenerants per callus, and the doubled haploids proportion, there were no statistically significant differences between the main and lateral panicles in the L×3P combination. In total, 215 doubled haploids lines of twenty nine genotypes were obtained, which is theoretically sufficient for breeding a new variety. On the hybrid combination K×3P, 120 doubled haploids of five genotypes were formed from one donor plant with the main panicle. There were no statistically significant differences between the main and lateral panicles. It is concluded that it is expedient to use lateral rice panicles in vitro androgenesis to create the initial breeding material. Their use makes it possible to distribute labor force over time and increase the total volume of anthers cultivated in vitro, and, ultimately, the doubled haploids number and variability of one sample. In the time absence and other restrictions, it is preferable to use the main panicle.

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High-Frequency Androgenic Green Plant Regeneration in Indica Rice for Accelerated Breeding

Cited by 3 publications

References 55 publications

Comparison of three different RNA extraction kits for transcriptome analysis of Indonesian rice (Oryza sativa L) explant for anther culture

Comparison of three different RNA extraction kits for transcriptome analysis of Indonesian rice (Oryza sativa L) explant for anther culture

Panicle Formation Order Affects Rice Oryza sativa L. in vitro Androgenesis

Panicle formation order influence in rice Oryza sativa L in vitro androgenesis

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