High FNDC1 expression correlates with poor prognosis in gastric cancer

Zhong, Meifeng; Zhang, Yijie; Yuan, Fangfang; Peng, Yuling; Wu, Jingjing; Yuan, Jiawei; Zhu, Wei; Zhang, Yali

doi:10.3892/etm.2018.6731

Cited by 10 publications

(9 citation statements)

References 41 publications

(57 reference statements)

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“…Furthermore, the expression of FNDC1 was not associated with DFS in patients with BC. By contrast, previous studies have suggested that the expression of FNDC1 was associated with prognosis in gastric cancer (11,14). These results suggested that the role of FNDC1 in tumors is complicated.…”

Section: Discussioncontrasting

confidence: 61%

The silencing of FNDC1 inhibits the tumorigenesis of breast cancer cells via modulation of the PI3K/Akt signaling pathway

et al. 2021

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Fibronectin type III domain-containing protein 1 (FNDC1) is a protein that contains a major component of the structural domain of fibronectin. Although many studies have indicated that FNDC1 serves vital roles in the development of various diseases, the role of FNDC1 in the progression of breast cancer (BC) remains elusive. The aim of the present study was to investigate the biological functions of FNDC1 in BC cells and the associated mechanisms. The expression levels of FNDC1 in BC tissues and normal breast tissues were analyzed using The Cancer Genome Atlas database (TCGA). Kaplan-Meier curves were mined from TCGA to examine the clinical prognostic significance of FNDC1 mRNA in patients with BC. The expression of FNDC1 was knocked down by transfection with shRNA in BC cells. Cell viability, colony formation ability, migration and invasion were assayed following the silencing of FNDC1 in BC cells. The expression of proteins was measured using a western blotting assay. The bioinformatic data indicated that the FNDC1 mRNA expression levels were significantly upregulated in BC tissues compared with normal breast tissues, and the high mRNA expression levels of FNDC1 were associated with a lower overall survival in patients with BC. The downregulation of FNDC1 inhibited the proliferation, colony formation, migration and invasion of BC cells. Investigation of the mechanisms revealed that the silencing of FNDC1 decreased the protein expression levels of MMPs and epithelial-to-mesenchymal markers. Furthermore, the silencing of FNDC1 led to the inactivation of the PI3K/Akt signaling pathway. FNDC1 was highly upregulated and acted as an oncogene in BC. Therefore, targeting FNDC1 may be a potential strategy for the treatment of BC.

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Section: Discussioncontrasting

confidence: 61%

The silencing of FNDC1 inhibits the tumorigenesis of breast cancer cells via modulation of the PI3K/Akt signaling pathway

et al. 2021

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“…Some scholars have found that the upregulation of microRNA-1207-3p can significantly inhibit the migration and proliferation of tumors in the study of prostate cancer, further verifying that it can induce tumor cell apoptosis by regulating the downstream target gene FNDC1 (7). Recent studies have also reported a correlation between the expression of FNDC1 in GC and clinical characteristics of patients, reporting that the overexpression of FNDC1 is associated with poor prognosis of patients (8)(9)(10). In our previous study (4), molecular markers of PM were analyzed by WES and FNDC1 was found to have a higher frequency of mutations in PM patients, suggesting that FNDC1 may be associated with GC invasion and PM.…”

Section: Discussionmentioning

confidence: 92%

“…Several studies have found that FNDC1 is closely related to the development of many diseases including various tumors (5)(6)(7). Recent studies have reported a correlation between the expression of FNDC1 in GC and clinical characteristics of patients (8)(9)(10). However, the exact role of FNDC1 in GC and the potential molecular mechanism has not been fully elucidated, and the effect of FNDC1 on PM remains to be revealed.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: FNDC1 Promotes the Invasiveness of Gastric Cancer via Wnt/β-Catenin Signaling Pathway and Correlates With Peritoneal Metastasis and Prognosis

et al. 2020

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BackgroundGastric cancer (GC) has a high morbidity and mortality rate, with peritoneal metastasis (PM) identified as the main site of metastasis. Our previous study found that FNDC1 has a higher frequency of mutations in patients with PM by high-throughput sequencing assay, suggesting that it may be associated with GC invasion and PM, however the specific mechanism remains unclear.MethodsFirst, the correlation between FNDC1 and PM and prognosis of GC was clarified by bioinformatics and clinicopathological analysis. Next, the effect of FNDC1 expression on the invasion and metastasis ability of GC was investigated in vivo and in vitro. Finally, the signaling pathways involved in the regulation of FNDC1 were explored.ResultsFNDC1 was highly expressed in GC and was associated with PM and poor prognosis. FNDC1 was also associated with epithelial-mesenchymal transition (EMT) in GC cells. Through in vivo and in vitro experiments, it was clarified that knockdown of FNDC1 could inhibit the proliferation, invasion, and migration of GC cells. In addition, it was elucidated that FNDC1 promotes EMT through the Wnt/β-catenin signaling pathway.ConclusionFNDC1 may be associated with the invasion of GC and PM after surgery. FNDC1 was highly expressed in GC tissues and cell lines, while significantly associated with poor DFS and OS in GC patients. Both univariate and multivariate analyses suggested that the expression of FNDC1 was an independent factor for GC. Knockdown of FNDC1 also significantly inhibited the proliferation, migration, and activity of GC cells. FNDC1 may promote EMT in GC cells through the regulation of Wnt/β-catenin signaling pathway. FNDC1 has the potential to be used as a predictor of PM and may also be studied in depth as a therapeutic target for GC, which has potential clinical utility and is worthy of further validation.

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“…FNDC1 was also shown to promote apoptosis through hypermethylation in human salivary-like cystic carcinoma cells [ 36 ]. Additionally, high FNDC1 expression was also reported to be associated with poor prognosis in gastric cancer [ 37 ], which is consistent with our findings. INHBA encodes a member of the TGF-beta superfamily of proteins, which has been found to be associated with various types of human cancers.…”

Section: Discussionsupporting

confidence: 92%

Identification of hub genes and construction of an mRNA-miRNA-lncRNA network of gastric carcinoma using integrated bioinformatics analysis

Wei

Dong²,

He³

et al. 2021

PLoS ONE

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Background Gastric carcinoma (GC) is one of the most common cancer globally. Despite its worldwide decline in incidence and mortality over the past decades, gastric cancer still has a poor prognosis. However, the key regulators driving this process and their exact mechanisms have not been thoroughly studied. This study aimed to identify hub genes to improve the prognostic prediction of GC and construct a messenger RNA-microRNA-long non-coding RNA(mRNA-miRNA-lncRNA) regulatory network. Methods The GSE66229 dataset, from the Gene Expression Omnibus (GEO) database, and The Cancer Genome Atlas (TCGA) database were used for the bioinformatic analysis. Differential gene expression analysis methods and Weighted Gene Co-expression Network Analysis (WGCNA) were used to identify a common set of differentially co-expressed genes in GC. The genes were validated using samples from TCGA database and further validation using the online tools GEPIA database and Kaplan-Meier(KM) plotter database. Gene set enrichment analysis(GSEA) was used to identify hub genes related to signaling pathways in GC. The RNAInter database and Cytoscape software were used to construct an mRNA-miRNA-lncRNA network. Results A total of 12 genes were identified as the common set of differentially co-expressed genes in GC. After verification of these genes, 3 hub genes, namely CTHRC1, FNDC1, and INHBA, were found to be upregulated in tumor and associated with poor GC patient survival. In addition, an mRNA-miRNA-lncRNA regulatory network was established, which included 12 lncRNAs, 5 miRNAs, and the 3 hub genes. Conclusions In summary, the identification of these hub genes and the establishment of the mRNA-miRNA-lncRNA regulatory network provide new insights into the underlying mechanisms of gastric carcinogenesis. In addition, the identified hub genes, CTHRC1, FNDC1, and INHBA, may serve as novel prognostic biomarkers and therapeutic targets.

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High FNDC1 expression correlates with poor prognosis in gastric cancer

Cited by 10 publications

References 41 publications

The silencing of FNDC1 inhibits the tumorigenesis of breast cancer cells via modulation of the PI3K/Akt signaling pathway

The silencing of FNDC1 inhibits the tumorigenesis of breast cancer cells via modulation of the PI3K/Akt signaling pathway

RETRACTED: FNDC1 Promotes the Invasiveness of Gastric Cancer via Wnt/β-Catenin Signaling Pathway and Correlates With Peritoneal Metastasis and Prognosis

Identification of hub genes and construction of an mRNA-miRNA-lncRNA network of gastric carcinoma using integrated bioinformatics analysis

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