High fluorescence quenching probe-based reverse fluorescence enhancement LFTS coupling with IS-primer amplification reaction for the rapid and sensitive Parkinson Disease-associated MicroRNA detection

Chen, Ming‐Hui; Li, Hengxuan; Shi, Zhihong; Peng, Weipan; Qin, Yi; Luo, Ran; Zhou, Dianming; Gong, Xiaoqun; Chang, Jin

doi:10.1016/j.bios.2020.112278

Cited by 29 publications

(14 citation statements)

References 41 publications

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“…[366] In recent work, miRNA detection was achieved for PD by coating AuNPs with black hole quencher 2 (BHQ2) labeling hairpin DNA (AuNP@hDNA-BHQ2), which showed a quick (35 min) and highly sensitive (fM level) response to miR-5010 and miR-331. [367] Notably, the properties of AuNPs, including cellular internalization, biodistribution, gene delivery efficiency, and optical properties, may change dramatically when the size and/or the shape of spherical AuNPs change. This should be taken into consideration before using AuNPs to deliver gene modulators.…”

Section: Gold Npsmentioning

confidence: 99%

“…[ 363 , 364 ] Indeed, AuNPs have been widely used in biosensor development for disease‐implicating genes detection in AD [ 365 , 366 ] and PD. [ 367 ] For example, Delkhahi et al developed an enzyme‐free biosensor for the detection and quantification of miR‐137 by combining AuNPs‐based colorimetric detections and DNA hybridization chain reaction (HCR) amplification. This biodevice allowed for a high sensitivity within the linear range of 0.25–5 n m .…”

Section: Nanomedicines Used In Gene Delivery For Cns Disease Treatmentmentioning

confidence: 99%

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Delivering the Promise of Gene Therapy with Nanomedicines in Treating Central Nervous System Diseases

et al. 2022

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Central Nervous System (CNS) diseases, such as Alzheimer's diseases (AD), Parkinson's Diseases (PD), brain tumors, Huntington's disease (HD), and stroke, still remain difficult to treat by the conventional molecular drugs. In recent years, various gene therapies have come into the spotlight as versatile therapeutics providing the potential to prevent and treat these diseases. Despite the significant progress that has undoubtedly been achieved in terms of the design and modification of genetic modulators with desired potency and minimized unwanted immune responses, the efficient and safe in vivo delivery of gene therapies still poses major translational challenges. Various non‐viral nanomedicines have been recently explored to circumvent this limitation. In this review, an overview of gene therapies for CNS diseases is provided and describes recent advances in the development of nanomedicines, including their unique characteristics, chemical modifications, bioconjugations, and the specific applications that those nanomedicines are harnessed to deliver gene therapies.

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Section: Gold Npsmentioning

confidence: 99%

Section: Nanomedicines Used In Gene Delivery For Cns Disease Treatmentmentioning

confidence: 99%

Delivering the Promise of Gene Therapy with Nanomedicines in Treating Central Nervous System Diseases

et al. 2022

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“…[31] In addition to the abovementioned examples, BHQs have also been used in the detection of ATP, [32] esterase, [33] DNA topoisomerase, [34] Uracil DNA glycosylases (Ung), [35] nicking enzyme, [36] conformational changes of ribosome, [37] DNA methylation, [38] MMP-13, [39] cell differentiation of mesenchymal stem cells (MSCs), [40] hypoxic tissues, [41] and Parkinson's disease (PD) biomarkers. [42] [26] with permission. Copyright 2021, American Chemical Society.…”

Section: Black Hole Quenchers (Bhqs)mentioning

confidence: 99%

Small‐Molecule Quenchers for Förster Resonance Energy Transfer: Structure, Mechanism, and Applications

et al. 2022

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For fluorogenic probes, Förster resonance energy transfer (FRET) is one of the most widely used mechanisms to realize the detection of biochemical activities. Dark quenchers relax from the excited state to the ground state nonradiatively, and are promising alternatives to fluorescent FRET acceptors. Small-molecule (dark) quenchers have been widely used as acceptors in FRET-based probes to monitor various physiological processes with minimum background signal. Herein, we summarize the relevant advances of small-molecule quenchers that are used in FRET-based probes. This Review is intended to provide suggestions regarding the rationale design and selection of appropriate fluorophorequencher FRET pairs, which are fully compatible with challenging analytical applications in various biological systems. Finally, an outlook of the future biomedical applications and developments of this field is presented.

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“…Nanotechnology, with the improvement and application of the nano‐particles (NPs)/nanocarriers with dimension in the nanoscale range (1 ~ 100 nm) (Gong et al, 2018; Piao et al, 2019; B. Zhang, Gao, et al, 2018), has been widely utilized in a variety of fields, including medicine (Emerich & Thanos, 2003), pharmacy (Li et al, 2020), biological detection (Chen et al, 2020; J. Cui et al, 2020; Peng, Huang, et al, 2020), optics (Ochmann et al, 2017; Peng, Qin, et al, 2020), and agriculture (Curtis & Wilkinson, 2001; Prasad et al, 2017). The extremely small size of NPs allows efficient entrance into living system such as animal and human body.…”

Section: Introductionmentioning

confidence: 99%

Applications of nanotechnology in virus detection, tracking, and infection mechanisms

Kang

Tahir

Wang

et al. 2021

WIREs Nanomed Nanobiotechnol

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Viruses are among the most infectious pathogens, responsible for the highest death toll around the world. Lack of effective clinical drug for most of the viruses emphasizes the rapid and accurate diagnosis at early stages of infection to prevent rapid spread of the pathogens. Nanotechnology is an emerging field with applications in various domains, where nano‐biomedical science has many significant contributions such as effective delivery of drugs/therapeutic molecules to specific organs, imaging, sensitive detection of virus, and their accurate tracking in host cells. The nanomaterials reported for virus detection and tracking mainly include magnetic and gold NPs, ZnO/Pt‐Pd, graphene, and quantum dots (QDs). In addition, the single virus tracking technology (SVT) allowed to track the life cycle stages of an individual virus for better understanding of their dynamics within the living cells. Inorganic as well as non‐metallic fluorescent materials share the advantages of high photochemical stability, a wide range of light absorption curves and polychromatic emission. Hence, are considered as potential fluorescent nano‐probes for SVT. However, there are still some challenges: (i) clinical false positive rate of some detection methods is still high; (ii) in the virus tracking process, less adaptability of QDs owing to larger size, flicker, and possible interference with virus function; and (iii) in vivo tracking of a single virus, in real time needs further refinement. In the future, smaller, non‐toxic, and chemically stable nanomaterials are needed to improve the efficiency and accuracy of detection, and monitoring of virus infections to curb the mortalities. This article is categorized under: Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease Biology‐Inspired Nanomaterials > Protein and Virus‐Based Structures

show abstract

High fluorescence quenching probe-based reverse fluorescence enhancement LFTS coupling with IS-primer amplification reaction for the rapid and sensitive Parkinson Disease-associated MicroRNA detection

Cited by 29 publications

References 41 publications

Delivering the Promise of Gene Therapy with Nanomedicines in Treating Central Nervous System Diseases

Delivering the Promise of Gene Therapy with Nanomedicines in Treating Central Nervous System Diseases

Small‐Molecule Quenchers for Förster Resonance Energy Transfer: Structure, Mechanism, and Applications

Applications of nanotechnology in virus detection, tracking, and infection mechanisms

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