High-fluence rate responses in the light-oriented chloroplast movement in Adiantum protonemata

Yatsuhashi, Hiroko; Wada, Masamitsu

doi:10.1016/0168-9452(90)90156-i

Cited by 33 publications

(18 citation statements)

References 8 publications

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“…RT-PCR analysis was performed as described previously (11). Chloroplast photorelocation movement was analyzed by WBA in a growth chamber with a 3.6-kW xenon lamp (Ushio) and by the microbeam irradiation method with a custom-made system (24). Immunoblotting, fluorescent protein, and Y2H analyses are described in SI Materials and Methods.…”

Section: Methodsmentioning

confidence: 99%

Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis

Kodama

Suetsugu

Kong

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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120

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Chloroplasts move toward weak light (accumulation response) and away from strong light (avoidance response). The fast and accurate movement of chloroplasts in response to ambient light conditions is essential for efficient photosynthesis and photodamage prevention in chloroplasts. Here, we report that two Arabidopsis mutants, weak chloroplast movement under blue light 1 (web1) and web2, are defective in both the avoidance and the accumulation responses. Mapbased cloning revealed that both genes encode coiled-coil proteins and that WEB2 is identical to the plastid movement impaired 2 (PMI2) gene. The velocities of chloroplast movement in web1 and pmi2 were approximately threefold lower than that in the wild type. Defects in the avoidance response of web1 and pmi2 were suppressed by mutation of the J-domain protein required for chloroplast accumulation response 1 (JAC1) gene, which is essential for the accumulation response; these results indicate that WEB1 and PMI2 play a role in suppressing JAC1 under strong light conditions. A yeast two-hybrid analysis and a nuclear recruitment assay identified a physical interaction between WEB1 and PMI2, and transient expression analysis of CFP-WEB1 and YFP-PMI2 revealed that they colocalized in the cytosol. Bimolecular fluorescence complementation analysis confirmed the interaction of these proteins in the cytosol. Blue light-induced changes in short chloroplast actin filaments (cp-actin filaments) were impaired in both web1 and pmi2. Our findings suggest that a cytosolic WEB1-PMI2 complex maintains the velocity of chloroplast photorelocation movement via cp-actin filament regulation.Arabidopsis thaliana | chloroplast velocity | phototropin | protein-protein interaction

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Section: Methodsmentioning

confidence: 99%

Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis

Kodama

Suetsugu

Kong

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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120

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“…Plants were incubated on one-half-strength Gamborg's B5 agar medium (Gamborg et al, 1968) For microbeam irradiation, gemmalings were transferred to a custom-made cuvette (25 mm in diameter and 5 mm in height) that consisted of two sets of a steel ring and a round glass with a silicone-rubber ring spacer in the dark (Wada et al, 1983). The cuvette containing the gemmaling was placed on the sample stage of a microbeam irradiator (Yatsuhashi and Wada, 1990;Tsuboi et al, 2006;Wada, 2007). Microbeam irradiation was performed as previously described (Yatsuhashi and Wada, 1990;Tsuboi et al, 2006;Wada, 2007) with some modifications.…”

Section: Culture and Growth Conditions Of Marchantia Polymorphamentioning

confidence: 99%

“…The cuvette containing the gemmaling was placed on the sample stage of a microbeam irradiator (Yatsuhashi and Wada, 1990;Tsuboi et al, 2006;Wada, 2007). Microbeam irradiation was performed as previously described (Yatsuhashi and Wada, 1990;Tsuboi et al, 2006;Wada, 2007) with some modifications. The gemmalings were irradiated with a microbeam at different intensities of red or blue light.…”

Section: Culture and Growth Conditions Of Marchantia Polymorphamentioning

confidence: 99%

Phototropin Encoded by a Single-Copy Gene Mediates Chloroplast Photorelocation Movements in the LiverwortMarchantia polymorpha

et al. 2014

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Blue-light-induced chloroplast photorelocation movement is observed in most land plants. Chloroplasts move toward weak-lightirradiated areas to efficiently absorb light (the accumulation response) and escape from strong-light-irradiated areas to avoid photodamage (the avoidance response). The plant-specific kinase phototropin (phot) is the blue-light receptor for chloroplast movements. Although the molecular mechanisms for chloroplast photorelocation movement have been analyzed, the overall aspects of signal transduction common to land plants are still unknown. Here, we show that the liverwort Marchantia polymorpha exhibits the accumulation and avoidance responses exclusively induced by blue light as well as specific chloroplast positioning in the dark. Moreover, in silico and Southern-blot analyses revealed that the M. polymorpha genome encodes a single PHOT gene, MpPHOT, and its knockout line displayed none of the chloroplast photorelocation movements, indicating that the sole MpPHOT gene mediates all types of movement. Mpphot was localized on the plasma membrane and exhibited blue-light-dependent autophosphorylation both in vitro and in vivo. Heterologous expression of MpPHOT rescued the defects in chloroplast movement of phot mutants in the fern Adiantum capillus-veneris and the seed plant Arabidopsis (Arabidopsis thaliana). These results indicate that Mpphot possesses evolutionarily conserved regulatory activities for chloroplast photorelocation movement. M. polymorpha offers a simple and versatile platform for analyzing the fundamental processes of phototropin-mediated chloroplast photorelocation movement common to land plants.

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“…Prothallial cells were microbeam irradiated using an epimicrobeam irradiator (Yatsuhashi and Wada, 1990). The prothalli between agar-gelatin thin films were transferred into a cuvette composed of two round coverslips supported by a silicon-rubber, ring-shaped spacer (o.d.…”

Section: Microbeam Irradiationmentioning

confidence: 99%

“…Lightinduced chloroplast relocation can also be induced by partial cell irradiation by both LFR and HFR light. If a cell is partially irradiated with a beam of low-fluence blue light, the chloroplasts in the cell move toward the irradiated area; however, with a high-fluence beam they move away from the irradiated area (Yatsuhashi et al, 1985;Yatsuhashi and Wada, 1990).…”

mentioning

confidence: 99%

Chloroplast-Avoidance Response Induced by High-Fluence Blue Light in Prothallial Cells of the Fern Adiantum capillus-veneris as Analyzed by Microbeam Irradiation1

Kagawa

Wada

1999

Plant Physiology

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High-fluence rate responses in the light-oriented chloroplast movement in Adiantum protonemata

Cited by 33 publications

References 8 publications

Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis

Two interacting coiled-coil proteins, WEB1 and PMI2, maintain the chloroplast photorelocation movement velocity in Arabidopsis

Phototropin Encoded by a Single-Copy Gene Mediates Chloroplast Photorelocation Movements in the LiverwortMarchantia polymorpha

Chloroplast-Avoidance Response Induced by High-Fluence Blue Light in Prothallial Cells of the Fern Adiantum capillus-veneris as Analyzed by Microbeam Irradiation1

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