High fluence low‐power laser irradiation induces apoptosis via inactivation of Akt/GSK3β signaling pathway

Huang, Lei; Wu, Shengnan; Xing, Da

doi:10.1002/jcp.22367

Cited by 61 publications

(55 citation statements)

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“…At higher doses, a higher concentration of Ca 2+ is released causing hyperactivity of Ca 2+ -ATPase, an enzyme that promotes calcium influx, which exhausts the ATP reserves in the cell (Hawkins and Abrahamse, 2005), and thereby promotes cell death. Huang et al (2011) identified a new and important pro-apoptotic signalling pathway that utilized Akt/GSK3β inactivation by High fluence LLLT (HF-LLLT) (120 J/cm 2 , 633 nm) stimulation. The authors found that the activation of glycogen synthase kinase 3β (GSK3β) by HF-LLLT was due to the inactivation of protein kinase B (Akt), a widely reported and important upstream negative regulator of GSK3β.…”

Section: Discussionmentioning

confidence: 99%

“…Specific alterations in the cell compartment levels during cell death can be identified and measured. Mechanisms of cellular death can be initiated by diverse stimuli, including radiation (Hawkins and Abrahamse, 2005;Huang et al, 2011). …”

Section: Introductionmentioning

confidence: 99%

“…It is known that the mitochondria, a fundamental organelle for maintaining vital cellular functions, also plays a key role in cell death through the regulation of cytochromes (Lev-Tov et al, 2013a, b), intracellular Ca 2+ concentration (Rimessi et al, 2008), reactive oxygen species (ROS) (Huang et al, 2011;Sun et al, 2010;Wu et al, 2009), transmembrane mitochondrial potential (Belletti et al, 2015;Wu et al, 2009), mitochondrial transition pores by caspases or ATP depletion , changes in the redox state metabolism (Belletti et al, 2015) and cyclosporine A-sensitive mitochondrial permeability transition .…”

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of different doses of coherent light (laser) and non-coherent light (light-emitting diode) on cellular necrosis and apoptosis: a study in vitro

et al. 2016

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Introduction: Threshold doses of electromagnetic radiation can initiate necrosis and apoptosis in cells. The purpose of this study was to evaluate cellular apoptosis and necrosis immediately (t 0 ) and 24 hours (t 24 ) after irradiation with different doses of coherent light (laser) or non-coherent light (LED). Methods: CHO-K1 lineage cells were irradiated with laser (810nm) or LED (945±20nm), with 24mW, contact area of 1cm 2 and doses of 10, 20, 30, 40 and 50J/cm 2 for 300, 660, 960, 1230 and 1620s, respectively, at both wavelengths. Cells were evaluated by fluorescence microscopy, differentiating viable, apoptotic and necrotic cells immediately and 24 hours after irradiation. Results: The number of necrotic cells at t 0 was higher in the LED 40 and 50J/cm 2 groups (86±14 and 84±16% respectively, p <0.05), than in the 10 and 20J/cm 2 laser (5±2 and 5±3%, p<0.05) and LED (5±3 and 4±1%, p<0.05) conditions. At t 24, the LED 40J/cm 2 (80±20%, p<0.05) group also showed more necrosis than the control and lower dose groups (laser 10, 20, and 30J/cm 2 percentage of 6±4, 10±3 and 7±3%, p<0.05; LED 10 and 20J/cm 2 percentage of 3±1 and 17±10%, p<0.05). A decrease in apoptotic cells was observed in the laser group with doses of 10, 40, and 50J/cm 2 (6±4, 3±1 and 1±1% respectively, not significant), as well as in the LED 40J/cm 2 (2±2%, not significant) group versus control. The cells had a higher percentage of apoptosis cells in the control group and with laser doses of 10 and 30J/cm 2 (percentage of 20±1 and 20±4%, not significant), while only the LED 40J/cm 2 (10±10%, not significant) had a lower percentage compared the control group. Conclusion: Laser or LED stimulation promoted an increase in cell necrosis in a high energy density condition as characterized in a dose-dependent inhibition therapy. Laser or LED infrared irradiation in low doses (up to 20J/cm 2 ) reduced the percentage of apoptosis in CHO-K1 cells, while high doses (30J/cm 2 ) elevated apoptosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative analysis of different doses of coherent light (laser) and non-coherent light (light-emitting diode) on cellular necrosis and apoptosis: a study in vitro

et al. 2016

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“…Laser activates Glycogen synthase kinase 3β (GSK3), which triggers apoptosis. A high-level of ROS can also lead to inactivation of the Akt/GSK3β signaling pathaway [9]. As a result of down regulation of this signaling pathway, greater GSK3 level is observed leading to increased apoptosis.…”

Section: Modification Of Kinases Activitymentioning

confidence: 99%

The Use of Low-Level Energy Laser Radiation in Basic and Clinical Research

Rola¹,

Doroszko²,

Derkacz³

2014

Adv Clin Exp Med

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Laser radiation has specific attributes: monochromaticity, high coherence and polarization. These properties result in the extensive use of lasers in medicine. Laser devices can be assigned into three basic groups by means of their level of energy: high, medium and low energy. All of these types of radiation are used in medicine. However, the most commonly used, in basic science and clinical studies, is low-energy radiation. Molecular effects of low energy laser irradiation on cells are generally described as "fotobiostimulation" and "fotobiomodulation". These phenomena consequently lead to attempts to exploit this kind of radiation as a treatment method (low-level laser therapy-LLLT). Areas in which LLLT is used are: regenerative medicine (for healing wounds and ulcers); aesthetic medicine (to improve appearance of scars); dentistry (to accelerate healing of implants); physiotherapy (to reduce chronic pain syndromes), orthopedics (in bone healing) and cardiology (as a prevention of restenosis after percutaneous coronary intervention). This paper discusses the medical applications of LLLT which are used in daily clinical practice as well as those The laser was invented by Theodor Maiman in 1960 and since that time it is used in many different aspects of human life, including medicine. Lasers are distinguished from other light sources by their coherence, polarization and monochromaticity; therefore, they can transmit a wide range of energy.The physical process enabling the laser to function is called stimulated emission (laser is an acronym of light amplification by stimulated emission of radiation). There are many ways to classify laser devices. Lasers can be grouped by gain medium, wavelengths, modes of operation but the most useful way, from a medical point of view, is the division by energy level. And so by the amount of energy transmitted, the sources of laser radiation are divided into 3 basic groups: low, medium and high energy.There are many reports regarding the usefulness of full laser energy spectrum in clinical practice. High-energy radiation causes tissue destruction and therefore it is mainly applied in surgery. It is used for coagulation, cutting tissue, control of bleeding, destruction of tumors etc. Medium energy lasers are mainly used in oncology, especially as a part of photodynamic therapy. The low energy laser, among the all energy radiation groups, seems to have the widest range of applications in medicine. Despite much research, the exact mechanism action of low energy laser radiation on the human body is still unknown. Due to numerous studies on low-energy radiation, new therapeutic applications are being discovered and enrolled to clinical practice. The aim of this paper is to review the most common clinical applications of low-energy lasers.

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“…22 Brie°y, the extracted proteins were separated in SDS-polyacrylamide gels and transferred to polyvinylidene di°uoride (PVDF) membranes. The membranes were washed three times for 10 min each time with tris bu®ered saline with tween 20 (TBST) and incubated with indicated primary antibodies at 4 C overnight.…”

Section: Western Blot Analysismentioning

confidence: 99%

Photoactivation of GLUT4 translocation promotes glucose uptake via PI3-K/Akt2 signaling in 3T3-L1 adipocytes

Huang

Gong

Jiang

et al. 2014

J. Innov. Opt. Health Sci.

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Insulin resistance is a hallmark of the metabolic syndrome and type 2 diabetes. Dysfunction of PI-3K/Akt signaling was involved in insulin resistance. Glucose transporter 4 (GLUT4) is a key factor for glucose uptake in muscle and adipose tissues, which is closely regulated by PI-3K/Akt signaling in response to insulin treatment. Low-power laser irradiation (LPLI) has been shown to regulate various physiological processes and induce the synthesis or release of multiple molecules such as growth factors, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of intracellular signaling pathways. Nevertheless, it is unclear whether LPLI could promote glucose uptake through activation of PI-3K/Akt/GLUT4 signaling in 3T3L-1 adipocytes. In this study, we investigated how LPLI promoted glucose uptake through activation of PI-3K/Akt/GLUT4 signaling pathway. Here, we showed that GLUT4 was localized to the Golgi apparatus and translocated from cytoplasm to cytomembrane upon LPLI treatment in 3T3L-1 adipocytes, which enhanced glucose uptake. Moreover, we found that glucose uptake was mediated by the PI3-K/Akt2 signaling, but not Akt1 upon LPLI treatment with Akt isoforms gene silence and PI3-K/Akt inhibitors. Collectively, our results indicate that PI3-K/Akt2/GLUT4 signaling act as the key regulators for improvement of glucose uptake under LPLI treatment in 3T3L-1 adipocytes. More importantly, our¯ndings suggest that activation of PI3-K/Akt2/GLUT4 signaling by LPLI may provide guidance in practical applications for promotion of glucose uptake in insulin-resistant adipose tissue.

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High fluence low‐power laser irradiation induces apoptosis via inactivation of Akt/GSK3β signaling pathway

Cited by 61 publications

References 61 publications

Comparative analysis of different doses of coherent light (laser) and non-coherent light (light-emitting diode) on cellular necrosis and apoptosis: a study in vitro

Comparative analysis of different doses of coherent light (laser) and non-coherent light (light-emitting diode) on cellular necrosis and apoptosis: a study in vitro

The Use of Low-Level Energy Laser Radiation in Basic and Clinical Research

Photoactivation of GLUT4 translocation promotes glucose uptake via PI3-K/Akt2 signaling in 3T3-L1 adipocytes

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