High Field Magnetic Resonance Imaging Evaluation of Superparamagnetic Iron Oxide Nanoparticles in a Permanent Rat Myocardial Infarction

Chapon, Catherine; Franconi, Florence; Lemaire, Laurent; Marescaux, Laurent; Legras, Pierre; Saint‐André, Jean‐Paul; Denizot, B.; Jeune, J-J Le

doi:10.1097/01.rli.0000052979.96332.90

Cited by 37 publications

(18 citation statements)

References 25 publications

(20 reference statements)

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“…SPIO were also injected intraveneously to label macrophages, which infiltrated kidney grafts in rats (Beckmann et al 2003), brain infarctions (Kleinschnitz et al 2003) and cardiac infarction in vivo (Chapon et al 2003). Thus, these particles offer new ways to perform a labelling of macrophages in vivo.…”

Section: Cell Labelling For Mrimentioning

confidence: 99%

Noninvasive Cell Tracking

Kießling

2008

Molecular Imaging II

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Cell-based therapies may gain future importance in defeating different kinds of diseases, including cancer, immunological disorders, neurodegenerative diseases, cardiac infarction and stroke. In this context, the noninvasive localization of the transplanted cells and the monitoring of their migration can facilitate basic research on the underlying mechanism and improve clinical translation. In this chapter, different ways to label and track cells in vivo are described. The oldest and only clinically established method is leukocyte scintigraphy, which enables a (semi)quantitative assessment of cell assemblies and, thus, the localization of inflammation foci. Noninvasive imaging of fewer or even single cells succeeds with MRI after labeling of the cells with (ultrasmall) superparamagentic iron oxide particles (SPIO and USPIO). However, in order to gain an acceptable signal-to-noise ratio, at a sufficiently high spatial resolution of the MR sequence to visualize a small amount of cells, experimental MR scanners working at high magnetic fields are usually required. Nevertheless, feasibility of clinical translation has been achieved by showing the localization of USPIO-labeled dendritic cells in cervical lymph nodes of patients by clinical MRI.Cell-tracking approaches using optical methods are important for preclinical research. Here, cells are labeled either with fluorescent dyes or quantum dots, or transfected with plasmids coding for fluorescent proteins such as green fluorescent protein (GFP) or red fluorescent protein (RFP). The advantage of the latter approach is that the label does not get lost during cell division and, thus, makes imaging of proliferating transplanted cells (e.g., tumor cells) possible. In summary, there are several promising options for noninvasive cell tracking, which have different strengths and limitations that should be considered when planning cell-tracking experiments.

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Section: Cell Labelling For Mrimentioning

confidence: 99%

Noninvasive Cell Tracking

Kießling

2008

Molecular Imaging II

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“…Numerical simulations indicated a relaxation rate enhancement (⌬R 2b ‫␥214.0؍‬ ⅐ LMD) proportional to LMD, the local magnetic dose (the additional sample magnetization due to the SPIO particles), a quantity related to the concentration of contrast agent. Key words: steady state free precession; magnetic susceptibility; contrast agent; iron-oxide; relaxometry; magnetic resonance microscopy Magnetic resonance microscopy using magnetically labeled cells has been used to study a variety of cellular events in medical research including stem cell (1-4) and immune cell (5,6) migration, brain ischemia (7,8), and cancer (9 -11). One approach uses high resolution MRI to detect either single (12,13) or groups of cells labeled with paramagnetic contrast agents, such as superparamagnetic iron-oxide (SPIO).…”

mentioning

confidence: 99%

“…Magnetic resonance microscopy using magnetically labeled cells has been used to study a variety of cellular events in medical research including stem cell (1)(2)(3)(4) and immune cell (5,6) migration, brain ischemia (7,8), and cancer (9 -11). One approach uses high resolution MRI to detect either single (12,13) or groups of cells labeled with paramagnetic contrast agents, such as superparamagnetic iron-oxide (SPIO).…”

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confidence: 99%

Relaxometry model of strong dipolar perturbers for balanced‐SSFP: Application to quantification of SPIO loaded cells

Menon

Bowen

2006

Magnetic Resonance in Med

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Magnetic resonance microscopy using magnetically labeled cells is an emerging discipline offering the potential for nondestructive studies targeting numerous cellular events in medical research. The present work develops a technique to quantify superparamagnetic iron-oxide (SPIO) loaded cells using fully balanced steady state free precession (b-SSFP) imaging. An analytic model based on phase cancellation was derived for a single particle and extended to predict mono-exponential decay versus echo time in the presence of multiple randomly distributed particles. Numerical models verified phase incoherence as the dominant contrast mechanism and evaluated the model using a full range of tissue decay rates, repetition times, and flip angles. Numerical simulations indicated a relaxation rate enhancement (⌬R 2b ‫␥214.0؍‬ ⅐ LMD) proportional to LMD, the local magnetic dose (the additional sample magnetization due to the SPIO particles), a quantity related to the concentration of contrast agent. Key words: steady state free precession; magnetic susceptibility; contrast agent; iron-oxide; relaxometry; magnetic resonance microscopy Magnetic resonance microscopy using magnetically labeled cells has been used to study a variety of cellular events in medical research including stem cell (1-4) and immune cell (5,6) migration, brain ischemia (7,8), and cancer (9 -11). One approach uses high resolution MRI to detect either single (12,13) or groups of cells labeled with paramagnetic contrast agents, such as superparamagnetic iron-oxide (SPIO). Cell labeling using this strategy has been accomplished through both in vitro and in vivo approaches. In vitro labeling involves incubation of a desired cell line in culture with SPIO prior to i.v. (1,5) or tissuespecific injection (2,3), while in vivo (or active) labeling is accomplished through i.v. injection of SPIO particles that are preferentially taken up by a target cell population (14,15). For the vast majority of cell types and with each labeling technique, SPIO agents have been shown to be minimally disruptive to cell function (16) with few exceptions (17), and show great promise for revealing cellular contributions to early stages of pathology (7), as well as the therapeutic effectiveness of stem cell homing in regenerative medicine (2,3).To date, the majority of imaging studies involve a qualitative assessment of the hypo-or hyper-intensities observed in images of tissues containing SPIO-labeled cells. Quantitative measures of cellular migration may allow assessment of the relative effectiveness of pharmacological interventions, stem cell homing, or changes in the intensity of inflammatory response that are typically associated with the progression of pathology. Detection of SPIO labeled cells has been accomplished through T 1 (9,18), T 2 (19,20) and TЈ 2 -weighted (21-23) acquisitions. TЈ 2 -weighted gradient echo acquisitions provide the greatest sensitivity to the presence of SPIO; however, these measurements are also sensitive to background field inhomogeneities induced by im...

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“…More detailed syntheses and modifications to the basic protocol are described in our recent work on size-controlled synthesis of these nanoparticles [69]. The relaxivity values for the 5 wt% dextran sulfate doped particles [anionic dextran iron oxide (ADIO)] were comparable to a literature dextran coated iron oxide preparation (SPIO) at 7 T [86]. Structural analysis by transmission electron microscope (TEM) (Fig.…”

Section: ) Targeting Of Mal-bsa Probe To Macrophages In Vitromentioning

confidence: 83%

The Integration of Positron Emission Tomography With Magnetic Resonance Imaging

Cherry¹,

Louie

Jacobs

2008

Proc. IEEE

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| A number of laboratories and companies are currently exploring the development of integrated imaging systems for magnetic resonance imaging (MRI) and positron emission tomography (PET). Scanners for both preclinical and human research applications are being pursued. In contrast to the widely distributed and now quite mature PET/computed tomography technology, most PET/MRI designs allow for simultaneous rather than sequential acquisition of PET and MRI data. While this offers the possibility of novel imaging strategies, it also creates considerable challenges for acquiring artifact-free images from both modalities. This paper discusses the motivation for developing combined PET/MRI technology, outlines the obstacles in realizing such an integrated instrument, and presents recent progress in the development of both the instrumentation and of novel imaging agents for combined PET/MRI studies. The performance of the first-generation PET/MRI systems is described. Finally, a range of possible biomedical applications for PET/MRI are outlined.

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High Field Magnetic Resonance Imaging Evaluation of Superparamagnetic Iron Oxide Nanoparticles in a Permanent Rat Myocardial Infarction

Abstract: Nanoparticle injection made it possible to discriminate normal from infarcted myocardium on T2-weighted images. However, the high magnetic field prevented the visualization of the T1 effect of SPIO nanoparticles.

Cited by 37 publications

References 25 publications

Noninvasive Cell Tracking

Noninvasive Cell Tracking

Relaxometry model of strong dipolar perturbers for balanced‐SSFP: Application to quantification of SPIO loaded cells

The Integration of Positron Emission Tomography With Magnetic Resonance Imaging

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