High field BOLD response to forepaw stimulation in the mouse

Adamczak, Joanna; Farr, Tracy D.; Seehafer, Jörg U.; Kalthoff, Daniel; Hoehn, Mathias

doi:10.1016/j.neuroimage.2010.02.083

Cited by 90 publications

(84 citation statements)

References 30 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent study [1] performed on mice using electrical forepaw stimulation claims the α2-adrenergic receptor agonist medetomidine to be better for long time studies than other anesthetics. However, the occurrence of BOLD activation in less than 60% of all scans performed (our study: > 95%) and the noisy temporal BOLD profiles do not clearly show the superiority of medetomidine anesthesia.…”

Section: Discussionmentioning

confidence: 99%

Hyperalgesia by low doses of the local anesthetic lidocaine involves cannabinoid signaling: An fMRI study in mice

Bosshard

Grandjean

Schroeter

et al. 2012

Pain

View full text Add to dashboard Cite

Functional magnetic resonance imaging (fMRI) using the blood oxygen level dependent (BOLD) contrast was used to study sensory processing in the brain of isoflurane anesthetized mice. The use of a cryogenic surface coil in a small-animal 9.4T system provided the sensitivity required for detection and quantitative analysis of hemodynamic changes caused by neural activity in the mouse brain in response to electrical forepaw stimulation at different amplitudes. A gradient echo-echo planar imaging (GE-EPI) sequence was used to acquire five coronal brain slices of 0.5 mm thickness. BOLD signal changes were observed in primary and secondary somatosensory cortices, the thalamus and the insular cortex, important regions involved in sensory and nociceptive processing. Activation was observed consistently bilateral despite unilateral stimulation of the forepaw. The temporal BOLD profile was segregated into two signal components with different temporal characteristics. The maximum BOLD amplitude of both signal components correlated strongly with the stimulation amplitude. Analysis of the dynamic behavior of the somatosensory 'fast' BOLD component revealed a decreasing signal decay rate constant koff with increasing maximum BOLD amplitude (and stimulation amplitude). This study demonstrates the feasibility of a robust BOLD fMRI protocol to study nociceptive processing in isoflurane anesthetized mice. The reliability of the method allows for detailed analysis of the temporal BOLD profile and for investigation of somatosensory and noxious signal processing in the brain, which is attractive for characterizing genetically engineered mouse models. AbstractFunctional magnetic resonance imaging (fMRI) using the blood oxygen level dependent (BOLD) contrast was used to study sensory processing in the brain of isoflurane anesthetized mice. The use of a cryogenic surface coil in a small-animal 9.4T system provided the sensitivity required for detection and quantitative analysis of hemodynamic changes caused by neural activity in the mouse brain in response to electrical forepaw stimulation at different amplitudes. A gradient echo-echo planar imaging (GE-EPI) sequence was used to acquire five coronal brain slices of 0.5 mm thickness. BOLD signal changes were observed in primary and secondary somatosensory cortices, the thalamus and the insular cortex, important regions involved in sensory and nociceptive processing. Activation was observed consistently bilateral despite unilateral stimulation of the forepaw. The temporal BOLD profile was segregated into two signal components with different temporal characteristics. The maximum BOLD amplitude of both signal components correlated strongly with the stimulation amplitude. Analysis of the dynamic behavior of the somatosensory 'fast' BOLD component revealed a decreasing signal decay rate constant k off with increasing maximum BOLD amplitude (and stimulation amplitude).This study demonstrates the feasibility of a robust BOLD fMRI protocol to study nociceptive processing in isofluran...

show abstract

Section: Discussionmentioning

confidence: 99%

Hyperalgesia by low doses of the local anesthetic lidocaine involves cannabinoid signaling: An fMRI study in mice

Bosshard

Grandjean

Schroeter

et al. 2012

Pain

View full text Add to dashboard Cite

show abstract

“…Functional connectivity maps from rats anaesthetized with α-chloralose are more similar to the ones observed under medetomidine than those seen under isoflurane; however, they exhibit localization to a lesser extent [37]. Therefore, medetomidine is currently the preferred choice [23,26,28,39,40,41]. …”

Section: Translational Functional Methodsmentioning

confidence: 99%

Advantages and Challenges of Small Animal Magnetic Resonance Imaging as a Translational Tool

Hoyer¹,

Gass

Weber‐Fahr

et al. 2014

Neuropsychobiology

View full text Add to dashboard Cite

The utilization of magnetic resonance imaging (MRI) methods in rodent models of psychiatric disorders provides considerable benefits for the identification of disease-associated brain circuits and metabolic changes. In this review, we discuss advantages and challenges of animal MRI and provide an overview of the major structural (voxel-based morphometry and diffusion tensor imaging) and functional approaches [resting-state functional MRI (rs-fMRI), MR spectroscopy (MRS), regional cerebral blood volume measurement and arterial spin labelling] that are applied in animal MRI research. The review mainly focuses on rs-fMRI and MRS. Finally, we take a look at some recent developments and refinements in the field.

show abstract

“…In the last years several alternatives have been presented: Medetomidine (Weber et al, 2006), (Adamczak et al, 2010), Propofol (Lahti et al, 1999), Etomidate and Sevoflurane. Medetomidine can be used for long scanning sessions and has produced a consistent BOLD response in SI, SII and thalamus, similar in magnitude to those of AC experiments (Pawela et al, 2009).…”

Section: Discussionmentioning

confidence: 99%