High Fertilization and Implantation Rates After Intracytoplasmic Sperm Injection

Steirteghem, A.C. Van; Nagy, Zsolt; Joris, Hubert; Liu, Jiaen; Staessen, Cathérine; Smitz, Johan; Wisanto, A.; Devroey, Paul

doi:10.1097/00006254-199402000-00020

Cited by 285 publications

(342 citation statements)

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“…The ICSI procedure was carried out in accordance with Van Steirteghem et al [14]. An inverted microscope with ×200 magnification (Olympus IX70, Tokyo, Japan) and Hoffman modulation contrast (Modulation Optics inc., Greenvale, NY, USA) were employed.…”

Section: Methodsmentioning

confidence: 99%

Does first polar body morphology predict oocyte performance during ICSI treatment?

Younis

Radin

Izhaki

et al. 2009

J Assist Reprod Genet

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Purpose To gain insight into the morphology of the first polar body (1 PB) in ICSI patients and to explore whether it could predict mature oocyte viability and performance in this setting. Methods Seventy two consecutive women planned to perform ICSI treatment were prospectively recruited for this study. All oocytes retrieved underwent evaluation for nuclear maturity and accurate assessment of 1 PB morphology. MII oocytes were cultured in separate groups in each woman in accordance with two different categories of 1 PB morphology. Category A included normal intact round or ovoid 1 PB and category B included abnormal fragmented 1 PB. Each oocyte was followed throughout fertilization, embryo cleavage and embryo transfer. Cycles that reached embryo transfer, were divided into three groups in accordance with 1 PB morphology. Group I included only category A 1 PB embryos, group II included categories A and B 1 PB embryos, whereas group III included only category B 1 PB embryos. Results A total of 687 oocytes were aspirated and 553 MII oocytes underwent ICSI leading to 410 zygotes showing normal fertilization on day one. Three hundred ninety seven embryos cleaved on day two and a total of 176 embryos were replaced into the uterus. Clinical implantation and pregnancy rates were significantly correlated with the morphology of the 1 PB corresponding to 31%, 9% and 2% and 61%, 24% and 5%, in groups I, II and III respectively. Conclusions Our findings demonstrate that 1 PB morphology is related to mature oocyte viability and it has the potential to predict oocyte performance and pregnancy achievement in infertile women undergoing ICSI treatment.

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Section: Methodsmentioning

confidence: 99%

Does first polar body morphology predict oocyte performance during ICSI treatment?

Younis

Radin

Izhaki

et al. 2009

J Assist Reprod Genet

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“…Intracytoplasmic sperm injection was performed in all patients according to the published procedures [10,11]. The holding and injection pipettes were made from glass capillaries with an outside diameter of 1 mm and inside diameter of 0.6 mm.…”

Section: Methodsmentioning

confidence: 99%

Recombinant HCG for triggering ovulation increases the rate of mature oocytes in women treated for ICSI

Farrag¹,

Costantini²,

Manna³

et al. 2008

J Assist Reprod Genet

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Purpose To conduct a prospective randomized study in order to investigate the effect of recombinant HCG (rHCG) on oocyte nuclear and cytoplasm maturity compared to urinary HCG (uHCG), for inducing ovulation in women treated with ICSI for male factor infertility. Materials and Methods We compared 89 patients randomly assigned to one of the two study groups. Group A consisted of 42 women who received a subcutaneous (s.c.) injection of 250 μg rHCG and group B consisted of 47 patients receiving an intramuscular (i.m.) injection of 10,000 IU uHCG. Results Patients treated with rHCG showed a rate of metaphase II oocytes, a number of metaphase II oocytes with mature cytoplasm and a rate of metaphase II oocytes with mature cytoplasm calculated from total MII oocytes statistically higher than in patients treated with uHCG. However this differences were not associated with a significantly better clinical outcome. Conclusion Our data show that in women treated with ICSI for male factor infertility, rHCG increases the rate of metaphase II oocytes, the number and the rate of MII oocytes with mature cytoplasm compared to uHCG. A larger study comparing transfer cycles of embryos all derived from oocytes with mature cytoplasm and transfer cycles of embryos all derived from oocytes with immature cytoplasm may be needed to clarify clinical correlations.

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“…Conventional PVP-ICSI procedure was executed as described elsewhere [10]. Fertilization and embryo development were examined by inverted microscope.…”

Section: Ha-icsimentioning

confidence: 99%

Efficiency of hyaluronic acid (HA) sperm selection

Parmegiani

Cognigni

Ciampaglia

et al. 2009

J Assist Reprod Genet

115

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High Fertilization and Implantation Rates After Intracytoplasmic Sperm Injection

Cited by 285 publications

References 0 publications

Does first polar body morphology predict oocyte performance during ICSI treatment?

Does first polar body morphology predict oocyte performance during ICSI treatment?

Recombinant HCG for triggering ovulation increases the rate of mature oocytes in women treated for ICSI

Efficiency of hyaluronic acid (HA) sperm selection

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