High-fat diet effect on periapical lesions and hepatic enzymatic antioxidant in rats

Tibúrcio-Machado, Camilla dos Santos; Lang, Pauline Mastella; Campos, Maria Martha; Wolle, Carlos Frederico Brilhante; Barcelos, Raquel Cristine Silva; Zago, Natália Brezolin; Leite, Carlos Eduardo; Etges, Adriana; Bier, Carlos Alexandre Souza

doi:10.1016/j.lfs.2020.118637

Cited by 6 publications

(4 citation statements)

References 58 publications

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“…In the present study, although hyperlipidaemia aggravated periapical bone destruction, it did not affect its oxidative stress parameters. This finding is consistent with previous studies, where Tibúrcio-Machado et al found that the area of AP lesions was larger in rats fed with a high-fat diet than in rats fed with a normal diet (Tibúrcio-Machado et al, 2021). However, there were no differences between the two groups in the histological intensity of local inflammation.…”

Section: Discussionsupporting

confidence: 93%

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Is oxidative stress involved in the hepatic inflammatory response to apical periodontitis? A comparative study in normal and hyperlipidaemic rat

Xiao

Lei

et al. 2023

Int Endodontic J

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Aim The aim of the study was to explore the involvement of oxidative stress (OS) in the hepatic inflammation induced by apical periodontitis (AP). Periapical, systemic and hepatic reaction to AP under hyperlipidaemia was also investigated. Methodology A total of 16 male Sprague–Dawley rats were fed with a hyperlipidaemic diet (HD) whereas another 16 rats with a normal diet (ND). After 9 weeks, the first molars of the right maxilla and mandible of 8 HD and 8 ND rats were exposed to induce AP (ND, ND + AP, HD and HD + AP group). After 5 weeks, rats were euthanized, the haematological tissue was collected directly from the heart, and serum levels of inflammatory cytokines were measured. Liver tissue was analysed by haematoxylin–eosin and Masson staining, and reverse transcription–polymerase chain reaction (RT‐PCR) was performed to detect mRNA expression of inflammatory cytokines. Serum, periapical, and hepatic OS parameters including total oxidant status (TOS), total antioxidant capacity (TAOC) and oxidative stress index (OSI) were measured by enzyme‐linked immunosorbent assay (ELISA). The area of AP lesion in the right maxilla or mandible was radiographically assessed. Student's t‐test was performed on the periapical data. A one‐way analysis of variance and the Kruskal–Wallis test were analysed for others. Results The HD + AP group had a larger AP lesion in the maxilla, compared with the ND + AP group (p < .05). The ND + AP group presented higher serum interleukin (IL)‐18, IL‐1β, TOS, OSI levels, lower serum TOAC levels, higher hepatic tumour necrosis factor (TNF)‐α mRNA expression and higher hepatic TOS, and OSI levels, compared with the ND group (p < .05). The HD + AP group had lower serum IL‐4 level, higher serum IL‐1β level, and higher hepatic IL‐6 and transforming growth factor (TGF) ‐β1 mRNA expression, compared with the HD group (p < .05). Conclusions Apical periodontitis could activate systemic and liver inflammation by promoting serum IL‐18, 1L‐1β, TOS and OSI expression, enhancing hepatic TOS and OSI expression and inhibiting serum TOAC expression. AP under hyperlipidaemia led to more profound periapical bone destruction in the maxilla and elicit systemic and liver inflammatory responses through elevating serum levels of IL‐1β, descending serum IL‐4 level and improving hepatic IL‐6 and TGF‐β1 expression.

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Section: Discussionsupporting

confidence: 93%

“…However, there were no differences between the two groups in the histological intensity of local inflammation. The high‐fat diet prolonged the inflammatory process but did not exacerbate the inflammation (Tibúrcio‐Machado et al, 2021). In our study, HD may have a similar effect on the oxidative stress of periapical tissue.…”

Section: Discussionmentioning

confidence: 99%

Is oxidative stress involved in the hepatic inflammatory response to apical periodontitis? A comparative study in normal and hyperlipidaemic rat

Xiao

Lei

et al. 2023

Int Endodontic J

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“…The changes promoted by HFD are known environmental factors that can change susceptibility to oral and systemic diseases. Studies have demonstrated the effects of the association of AP with HFD, and Tibúrcio‐Machado et al (2020) showed a reduction in CAT levels in groups fed HFD with AP. Conti et al (2020) demonstrated that in atherosclerotic animals induced by HFD, the induction of AP increased TG levels, greater intensity of the inflammatory infiltrate and greater alveolar bone loss.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, the pulp canal was exposed for 100 days, which is unprecedented when compared with other studies with durations of 28 days (Tibúrcio-Machado et al, 2020), 30 days (Cintra et al, 2016;Pereira et al, 2017) and 60 days (Tavares et al, 2021). Chronic AP is an inflammatory disorder of the periapical tissues that probably originates from the cariogenic involvement of the dental pulp (Chen et al, 2021), which is often not diagnosed because of its clinical symptoms or radiographic limitations.…”

Section: Groupsmentioning

confidence: 99%

Melatonin decreases plasma TNF‐α and improves nonenzymatic antioxidant defence and insulin sensitivity in rats with apical periodontitis fed a high‐fat diet

et al. 2022

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Aim To analyse the effects of melatonin (ME) treatment on oxidative stress and insulin resistance (IR) in rats with apical periodontitis (AP) fed a high‐fat diet (HFD). Methodology Eighty 60‐day‐old rats were divided into eight groups: control (CN), AP, HFD with AP (HFDAP), control with ME (CNME), AP with ME (APME), HFD with ME (HFDME) and HFD with AP+ME (HFDAPME). The animals from the HFD groups were fed a HFD throughout the experimental period. On day 7, the animals from the AP groups were subjected to experimental AP, and after 70 days, the ME groups were treated for 30 days. Glycaemia, insulinaemia, homeostatic model assessment for IR index, tumour necrosis factor‐α (TNF‐α), and interleukin‐6 were analysed in plasma using biochemical tests and enzyme‐linked immunosorbent assay. Thiobarbituric acid‐reactive substances (TBARS), carbonyl protein (CP), superoxide dismutase (SOD), catalase, glutathione peroxidase, glutathione (GSH) and total antioxidant capacity (ferric reducing antioxidant power [FRAP]) were analysed in the gastrocnemius muscle. Results (1) Association of AP and HDF exacerbated IR, and ME treatment improved this alteration; (2) AP and HFD and their association showed increased TNF‐α, and ME reversed it; (3) TBARS increased in the AP and HFDAP groups, and ME reversed only in the group with the association of disease and diet; (4) CP increased in all HFD groups and improved in the ME groups; (5) GSH activity decreased in all experimental groups, and ME increased this parameter only in the CN and AP groups; (6) FRAP did not change between the groups, but ME treatment increased its activity in the AP and HFD groups; (7) ME increased SOD in the CN and AP groups. Conclusion Apical periodontitis and HFD promoted IR, and the association of AP with diet promoted IR exacerbation; this resistance might have been caused by an increase in TNF‐α. AP promoted more intense changes in lipid oxidative damage than in protein oxidative damage. In non‐enzymatic antioxidant defence, it was observed that both AP and HFD and their association promoted a decrease in GSH levels. Overall, ME treatment reversed changes such as oxidative stress and IR.

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Experimental apical periodontitis alters salivary biochemical composition and induces local redox state disturbances in the salivary glands of male rats

Vazão,

Claudino,

Pimpinato

et al. 2024

Clin Oral Invest

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High-fat diet effect on periapical lesions and hepatic enzymatic antioxidant in rats

Cited by 6 publications

References 58 publications

Is oxidative stress involved in the hepatic inflammatory response to apical periodontitis? A comparative study in normal and hyperlipidaemic rat

Is oxidative stress involved in the hepatic inflammatory response to apical periodontitis? A comparative study in normal and hyperlipidaemic rat

Melatonin decreases plasma TNF‐α and improves nonenzymatic antioxidant defence and insulin sensitivity in rats with apical periodontitis fed a high‐fat diet

Experimental apical periodontitis alters salivary biochemical composition and induces local redox state disturbances in the salivary glands of male rats

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