High Expression of the Newly Found Long Noncoding RNA Z38 Promotes Cell Proliferation and Oncogenic Activity in Breast Cancer

Deng, Rilin; Liu, Bin; Wang, Yan; Yan, Feng; Hu, Shifan; Wang, Hongcan; Wang, Tingting; Li, Bin; Deng, Xiyun; Xiang, Sheng; Yang, Yinke; Zhang, Jian

doi:10.7150/jca.13117

Cited by 36 publications

(33 citation statements)

References 51 publications

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“…For example, lncRNA MALAT1 was upregulated in breast cancer and MALAT1 suppression inhibited cell proliferation, migration, and invasion, promoted apoptosis and cell cycle arrest in breast cancer . LncRNA Z38 was identified to be highly expressed and Z38 silencing significantly inhibited cell proliferation and tumorigenesis in breast cancer . Although great efforts have been made to elucidate the biological and mechanisms of lncRNAs in breast cancer, the pathogenesis of breast cancer is still unclear.…”

Section: Discussionsupporting

confidence: 52%

Long non-coding RNA H19 promotes the proliferation and invasion of breast cancer through upregulating DNMT1 expression by sponging miR-152

et al. 2017

J Biochem Mol Toxicol

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Long non-coding RNA (lncRNA) H19 in tumors played important roles in various biological processes. However, the biological role and molecular mechanism of H19 in breast cancer are unclear. Here, we found that H19 was aberrantly upregulated in human breast tumor tissues and cells. A negative correlation between H19 and miR-152 and positive correlation between H19 and DNMT1 mRNA were observed. Downregulation of H19 and DNMT1 significantly retarded breast cancer cell proliferation and invasion. H19 act as an endogenous sponge by directly binding to miR-152. miR-152 directly targeted DNMT1 and was regulated by H19. Besides, H19 overexpression dramatically relieved the inhibition of miR-152 on DNMT1 expression. miR-152 inhibition and DNMT1 overexpression obviously reversed the inhibitory effects of H19 downregulation on cell proliferation and invasion. In conclusion, H19 promoted proliferation and invasion of breast cancer through the miR-152/DNMT1 axis, providing a novel mechanism about the occurrence and development of breast cancer.

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Section: Discussionsupporting

confidence: 52%

Long non-coding RNA H19 promotes the proliferation and invasion of breast cancer through upregulating DNMT1 expression by sponging miR-152

et al. 2017

J Biochem Mol Toxicol

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“…The testis, epididymis, sperm, and seminal extracellular vesicles were lysed in RIPA buffer (20 mM sodium phosphate, 150 mM NaCl, pH 7.4, 1% Nonidet P‐40, 0.11% SDS, and 0.5% deoxycholic acid) containing phenylmethylsulfonyl fluoride and protease inhibitor mixture (Roche, Switzerland). The total protein content in each sample was quantified using the Modified BCA Protein Assay kit (Sangon Biotech, China; Deng et al, ). Proteins were separated on SDS‐polyacrylamide gels and transferred to nitrocellulose membranes (Amersham Biosciences, Germany).…”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ

Lin

Liang

et al. 2019

Molecular Reproduction Devel

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Asthenozoospermia is a common cause of male infertility, but in most cases its etiology is unknown. The exocytic cell vesicles called seminal extracellular vesicles in the human seminal fluid have been reported to play a pivotal role in promoting the motility of spermatozoa, and functional disorder of seminal extracellular vesicles may cause male infertility. To determine whether abnormal seminal extracellular vesicles are involved in asthenozoospermia, the differential abundance proteins between normozoospermic (NSEV) and asthenozoospermic seminal extracellular vesicles (ASEV) samples were analyzed by iTRAQ coupled with two‐dimensional liquid chromatography–tandem mass spectrometry. A total of 3,699 proteins were identified in the seminal extracellular vesicles (false discovery rate <0.01). Overall, 11 proteins were significantly upregulated (>1.2) in ASEV and 80 were significantly downregulated (<0.833). Functional bioinformatic analysis showed that these proteins with differential abundance were mainly associated with transport, metabolism, and signal pathways. The changes of OPTN, SMYD2, EIF2B2, TRPV6, ACE, PRSS8, and PPAP2A in ASEV were verified by western blot analysis, and we found that the abundance of TRPV6 markedly reduced in the seminal extracellular vesicles and ejaculated spermatozoa of asthenozoospermic patients, which indicated trpv6 was important in sperm motility. This study provides deeper insight into the involvement of seminal extracellular vesicles in asthenozoospermia and should aid the search for novel biomarkers of male infertility.

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“…A novel lncRNA Z38 was found to have high expression in breast cancer [2]. Inhibition of lncRNA Z38 expression by gene silencing reported suppression of cell proliferation and tumorigenesis.…”

Section: Therapeutic Usementioning

confidence: 99%

“…Inhibition of lncRNA Z38 expression by gene silencing reported suppression of cell proliferation and tumorigenesis. Treatment with siRNAs has shown significant induction of apoptosis and inhibition of tumor growth indicating that Z38 can act as a therapeutic target and biomarker in carcinomas [2]. Similarly, knockdown of lncRNA Linc00974 is reported to inhibit the cell proliferation and invasion with activation of apoptosis and cell cycle arrest in hepatocellular carcinoma [4].…”

Section: Therapeutic Usementioning

confidence: 99%

LncRNA as a Therapeutic Target for Angiogenesis

Kumar

Goyal

2017

CTMC

212

152

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Background: Out of 3 billion base pairs in human genome only ~2% code for proteins; and out of 180,000 transcripts in human cells, about 20,000 code for protein, remaining 160,000 are non-coding transcripts. Most of these transcripts are more than 200 base pairs and constitute a group of long non-coding RNA (lncRNA). Many of the lncRNA have its own promoter, and are well conserved in mammals. Accumulating evidence indicates that lncRNAs act as molecular switches in cellular differentiation, movement, apoptosis, and in the reprogramming of cell states by altering gene expression patterns. However, the role of this important group of molecules in angiogenesis is not well understood. Angiogenesis is a complex process and depends on precise regulation of gene expression. Conclusion:Dysregulation of transcription during this process may lead to several diseases including various cancers. As angiogenesis is an important process in cancer pathogenesis and treatment, lncRNA may be playing an important role in angiogenesis. In support of this, lncRNA microvascular invasion in hepatocellular carcinoma (MVIH) has been shown to activate angiogenesis. Furthermore, lncRNA-Meg3-knockout mouse showed increased expression of vascular endothelial growth factor pathway genes and increased cortical microvessel density. Overall, there is strong evidence that lncRNA is an important class of regulatory molecule, and a number of studies have demonstrated that these can be targeted to change cellular physiology and functions. In this review, we have attempted to summarize these studies and elucidate the potential of this novel regulatory molecule as a therapeutic target.

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High Expression of the Newly Found Long Noncoding RNA Z38 Promotes Cell Proliferation and Oncogenic Activity in Breast Cancer

Cited by 36 publications

References 51 publications

Long non-coding RNA H19 promotes the proliferation and invasion of breast cancer through upregulating DNMT1 expression by sponging miR-152

Long non-coding RNA H19 promotes the proliferation and invasion of breast cancer through upregulating DNMT1 expression by sponging miR-152

Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ

LncRNA as a Therapeutic Target for Angiogenesis

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