High Expression of Tetraspanin 5 as a Prognostic Marker of Colorectal Cancer

Abstract

Cancer is a major disease and the leading cause of death worldwide, with colorectal cancer (CRC) being the third-most common cancer in Korea. The survival rate associated with CRC reduces as the disease stage increases. Therefore, its early detection and treatment can greatly increase patient survival rates. In this study, we identified the tetraspanin 5 (TSPAN5) gene as an important biomarker for predicting the prognosis of patients with CRC. A TMA slide was used for statistical analysis. pN and clinical stage were found to be significant factors according to chi-square analysis, whereas pT, pN, metastasis, clinical stage, and TSPAN5 expression were significant according to Cox regression analysis. In order to prove the usefulness of TSPAN5, which is overexpressed in patients with metastatic CRC, as a biomarker, proliferation, migration, invasion, and tumorigenicity were examined using cell lines inhibited using small interfering RNA. The evaluations confirmed that TSPAN5 suppression, in turn, suppressed proliferation, migration, invasion, and tumorigenesis, which are characteristic of cancer cells. Therefore, the evaluation of TSPAN5 expression may help observe the prognosis of CRC and determine an appropriate treatment method for patients with CRC.

Spatial Transcriptomic Profiling of Tetraspanins in Stage 4 Colon Cancer from Primary Tumor and Liver Metastasis

“…By forming protein complexes with various cell surface molecules, such as integrins and receptors, tetraspanins participate in the progression and metastasis of various cancers, including CC (Scheme S1, Supplementary File S1) [5,7,8]. In CC, various studies have shown specific expressions of some tetraspanins in CC cell lines and bulk tissues, indicating their potential as diagnostic markers and therapeutic Life 2024, 14, 126 2 of 16 targets [7,9,10]. Some tetraspanins, such as TSPAN8 and CD151, have been associated with advanced tumor stage, metastasis, and poor clinical outcomes [7,11,12].…”