High expression of RELP (Reg IV) in neoplastic goblet cells of appendiceal mucinous cystadenoma and pseudomyxoma peritonei

Heiskala, Kukka; Giles‐Komar, Jill; Heiskala, Marja; Andersson, Leif C.

doi:10.1007/s00428-005-0105-1

Cited by 25 publications

(19 citation statements)

References 19 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, REGIV was clearly increased in goblets in active disease and seemed to be secreted with the mucus. The observation that REGIV is located to goblets corresponds to previous findings by Kamarainen and coworkers [16], and a recent study on appendiceal mucinous cystadenoma and pseudomyxoma peritonei [17] also found REGIV in goblets and mucus. This suggests that REGIV is secreted and may have a luminal mode of action.…”

Section: Discussionsupporting

confidence: 89%

Activation of REG family proteins in colitis

Granlund

Beisvåg

Torp

et al. 2011

Scandinavian Journal of Gastroenterology

View full text Add to dashboard Cite

AimsTo do a genome-wide gene expression study of active and inactive ulcerative colitis and Crohn's disease (inflammatory bowel disease – IBD) and examine the most differentially expressed genes. As the study showed an extreme upregulation of all regenerating islet-derived genes (REG proteins) in active IBD, we further studied the expression of REGs on protein level in active and inactive IBD, as well as in non-IBD (pseudomembranous) colitis.MethodsMicroarray analysis was done on a total of 100 pinch biopsy samples from healthy controls and patients with Crohn's disease or ulcerative colitis. Tissue samples from IBD and pseudomembranous colitis were examined with routine histology and immunohistochemical analysis for REGIα, REGIV, DEFA6, and serotonin.ResultsREG mRNAs were up to 83 times overexpressed in diseased mucosa compared with mucosa from healthy individuals. REGIα and REGIV were overexpressed at immunohistochemistry and located to different mucosal cell types. REGIα was expressed in basal half of crypts, REGIV in mid and outer parts of crypts and in surface epithelium and seems to be stored in, and secreted from, goblets. Pseudomembranous colitis samples showed similar staining patterns, and some IBD samples stained REG positive without inflammation on routine histology.ConclusionsAll REG family mRNAs are upregulated in IBD. REGIα and REGIV have different cellular localization, possibly reflecting different biological functions. REG protein expression also in pseudomembranous colitis shows that REG family proteins are regulated in inflammatory injury and repair, not specifically for IBD as previously thought.

show abstract

Section: Discussionsupporting

confidence: 89%

Activation of REG family proteins in colitis

Granlund

Beisvåg

Torp

et al. 2011

Scandinavian Journal of Gastroenterology

View full text Add to dashboard Cite

show abstract

“…Three genes showed a characteristic upregulation in pCRCs and a subsequent downregulation in LMs: SPINK4 , MMP3 and REG4 . Whereas SPINK4 and REG4 are expressed in differentiated cells (goblet cells and neuroendocrine cells, respectively48 49), MMP3 is mainly expressed in the connective tissue, implicated in the turnover of extracellular matrix to allow cell migration 50. Why these three genes confer a possible tumour promoting function in primary tumours, whereas their expression seems to be dispensable in liver metastases, is unclear.…”

Section: Discussionmentioning

confidence: 99%

Expression of an ASCL2 related stem cell signature and IGF2 in colorectal cancer liver metastases with 11p15.5 gain

Stange

Engel

Longerich

et al. 2010

Gut

View full text Add to dashboard Cite

show abstract

“…Present knowledge of the mRNA expression patterns of the various REG s in human colonic mucosa is extremely limited, with only a few studies demonstrating the expression of REG1A and REG4 in colorectal cancer and pseudomyxoma peritonei of the appendix (Astrosini et al 2008; Heiskala et al 2006). In the present study, we have utilised an in situ hybridisation (ISH) method involving branched-chain DNA technology, enabling us, for the first time, to localise all human REGs in normal and inflamed colon.…”

Section: Introductionmentioning

confidence: 99%

REG gene expression in inflamed and healthy colon mucosa explored by in situ hybridisation

et al. 2013

View full text Add to dashboard Cite

The regenerating islet-derived (REG) gene family encodes a group of proteins highly expressed in several human pathologies, many of which are associated with epithelial inflammation. All human family members, namely REG1A, REG1B, REG3A and REG4, are closely related in genomic sequence and all are part of the c-type lectin superfamily. REGs are highly expressed during inflammatory bowel disease (IBD)-related colonic inflammation and the in vivo expression pattern of REG1A and REG4 has been localised by using immunohistochemistry. However, the function of the encoded proteins is largely unknown and the cellular localisation of REG expression during colonic inflammation has not been described. Therefore, we have used in situ hybridisation to demonstrate the cellular localisation of REG expression in healthy and diseased colonic mucosa. Samples drawn from an IBD cohort including both inflamed and un-inflamed colonic mucosa are described, as are samples from non-IBD inflammation and healthy controls. Immunohistochemistry against known cell-type markers on serial sections has localised the expression of REGs to metaplastic Paneth cells (REG1A, REG1B and REG3A) and enteroendocrine cells (REG4), with a marked expansion of expression during inflammation. The group of REGs can, based on gene expression patterns, be divided into at least two groups; REG1A, REG1B and REG3A with their expression focused in the crypt base spreading from Paneth cells and REG4 being more highly expressed towards the luminal face. This exploration of expression pattern forms provides the background for further exploration of REG function in the intestine.Electronic supplementary materialThe online version of this article (doi:10.1007/s00441-013-1592-z) contains supplementary material, which is available to authorized users.

show abstract

High expression of RELP (Reg IV) in neoplastic goblet cells of appendiceal mucinous cystadenoma and pseudomyxoma peritonei

Cited by 25 publications

References 19 publications

Activation of REG family proteins in colitis

Activation of REG family proteins in colitis

Expression of an ASCL2 related stem cell signature and IGF2 in colorectal cancer liver metastases with 11p15.5 gain

REG gene expression in inflamed and healthy colon mucosa explored by in situ hybridisation

Contact Info

Product

Resources

About