High expression of heat shock protein 105 predicts a favorable prognosis for patients with urinary bladder cancer treated with radical cystectomy

Kawai, Taketo; Enomoto, Yutaka; Morikawa, Teppei; Matsushita, Hirokazu; Kume, Haruki; Fukayama, Masashi; Yamaguchi, Hirotsugu; Kakimi, Kazuhiro; Homma, Yukio

doi:10.3892/mco.2013.203

Cited by 10 publications

(3 citation statements)

References 25 publications

(25 reference statements)

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“… 25 However, according to another study in which it was revealed that tumors with lymphovascular invasion and lymph node metastasis exhibit a tendency for lower HSP105 scores, low HSP105 expression may be associated with lymphovascular invasion and/or metastasis independent of the depth of tumor invasion, which is consistent with our results. 26 There was no relationship between Breslow thickness and HSP105 expression in our study.…”

Section: Discussioncontrasting

confidence: 64%

Expression of Heat Shock Protein 105 in Cutaneous Squamous Cell Carcinoma: Correlation with Clinicopathological Characteristics

Jia¹,

Li²,

Ye³

et al. 2021

CCID

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Background Heat shock proteins (HSPs), a group of heat stress proteins, are characterized by highly conserved properties. Malignant transformation is a cellular stress, and the expression of HSPs may be affected during this process. Heat shock protein 105 (HSP105) is a protective protein that has long been observed in many cancer types, but little attention has been given to cutaneous squamous cell carcinoma (CSCC). As such, the objectives of this study were to observe the expression of HSP105 on CSCC and evaluate its correlation with clinicopathological characteristics. Methods This retrospective study enrolled 60 patients with CSCC. The patients’ clinical data, including sex, age, tumor location, tumor type, and degree of pathological differentiation, were collected. The expression of HSP105 was measured by Western blot and immunohistochemical staining. Results HSP105 expression was decreased in CSCC (HSCORE=0.65 (0.30, 1.98)) compared with normal skin (HSCORE=2.20 (1.50, 2.80)) (P<0.001). These results were consistent with the Western blot analysis. HSP105 immunostaining of Bowen disease (HSCORE=1.28 (1.08, 2.40)) revealed higher expression than in verrucous carcinoma (HSCORE=0.30 (0.23, 0.85)), keratoacanthoma (HSCORE=0.53 (0.29, 0.93)) and acantholytic squamous cell carcinoma (HSCORE=0.53 (0.41, 0.68) (P<0.01)). Poorly differentiated CSCC showed significantly higher expression of HSP105. Conclusion Our study reveals for the first time that the expression of HSP105 is decreased in CSCC. We suggest that the molecular mechanisms underlying the differential expression of HSP deserve a more rigorous future study, the results of which might explain its role in carcinogenesis and its potential as a target for selective tumor therapy.

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Section: Discussioncontrasting

confidence: 64%

Expression of Heat Shock Protein 105 in Cutaneous Squamous Cell Carcinoma: Correlation with Clinicopathological Characteristics

Jia¹,

Li²,

Ye³

et al. 2021

CCID

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“…Previous studies have also revealed that HSP105 is overexpressed in a variety of human cancers; for example, the expression of HSP105 in rectal cancer in general is higher than that in rectal adenocarcinoma [ 23 ]. However, other studies have found that the prolonged survival of bladder cancer patients is dependent on higher HSP105 expression [ 24 ]. In skin tumors, basal cell carcinoma expresses weak levels of HSP105 or is negative, which is in contrast to the high expression in EMPD and metastatic SCC [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

HSP105 expression in cutaneous malignant melanoma: Correlation with clinicopathological characteristics

Chen

Qian

et al. 2021

PLoS ONE

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Background Heat shock proteins can protect against stress-associated cellular challenges, but they can also protect some tumors from human immune system monitoring. Heat shock protein 105 (HSP105/110) is a high molecular weight protein whose expression has been reported in many cancers, but few studies on its role in cutaneous malignant melanoma have been published. In this study, we analyzed the relationship between HSP105 expression and the clinicopathological characteristics of CMM. Methods This retrospective study included 91 patients with CMM. The clinicopathological characteristics of CMM patients, including age, lesion duration, location, pathological classification, Clark’s level, Breslow thickness, metastasis and recurrence, were collected. Immunohistochemical staining and Western blot analysis for HSP105 were performed. Pigmented nevi (n = 20) served as a control. The staining intensity and percentage of stained cells were expressed as a histochemical score (HSCORE). Results HSP105 was overexpressed in melanoma compared with nevi. Differences in the HSCORE between nevi (HSCORE = 1.05(0.15,1.50)) and CMM (HSCORE = 2.68(1.80,3.60)) were remarkable (P<0.001). Exposed site lesions, recurrent and metastatic lesions, nodular melanoma and lentigo maligna melanoma were closely associated with higher HSP105 expression (P = 0.011, P = 0.001 and P = 0.001, respectively). Moreover, no significant difference was observed in Clark’s level, Breslow thickness, or lesion duration (P>0.05). Conclusion HSP105 is overexpressed in CMM. Higher HSP105 expression in lesions is associated with different clinicopathological variables. HSP105 may be a potential target for the diagnosis, treatment and prognostic prediction of CMM.

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“…The downregulated genes found for heat shock includes HSPA1B, HSPA4 and HSPB7, and those of homeobox family were HOXA13 and HOXB13 (Supplementary Table 5). Expression of heat shock protein family A (Hsp70) member 1B (HSPA1B) and, heat shock protein family A (Hsp70) member 4 (HSPA4) as well as heat shock protein family B (small) member 7 (HSPB7) were reported to be frequently overexpressed in bladder cancer cell lines [43,44]. This is due to the remarkable differences in bladder cancer expression compared with normal urothelium [45], leading to an intense investigation and suggestion for biomarkers of bladder cancer treatment response and prognosis [46].…”

Section: Repression Of Stress Proteins and Transcriptional Genes In Tccsuppimentioning

confidence: 99%

Functional Transcriptome Analysis of Bladder Cancer Cell Lines Persistently Infected with Oncolytic Newcastle Disease Virus

Ahmad

Thirumorthy

Abdullah

et al. 2021

Preprint

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Background Newcastle disease virus (NDV) has been identified as an attractive virotherapeutic agent that targets various type of human cancers while leaving normal cells unharmed. Wild-type NDV strain AF2240 has been found to persistently infect a subpopulation of cancer cells in vitro, making the cells less susceptible to NDV-mediated oncolysis. It is proposed that transcriptome profiling of NDV persistently infected bladder cancer cell lines will provide insights to understand such occurrence by identifying specific pathways associated with NDV persistent infection due to transcriptomic dysregulation. Results Transcriptome profiling revealed a total of 63 and 134 differentially expressed genes (DEGs) from NDV persistently infected TCCSUPPi and EJ28Pi bladder cancer cells relative to their uninfected controls, respectively. Of the 63 DEGs identified for TCCSUPPi cells, 25 DEGs were upregulated (log2 fold-change ≥ 0) and 38 DEGs were downregulated (log2 fold-change ≤ 0). These genes were significantly enriched in the molecular function of calcium binding (GO:0005509) and DNA-binding transcription repressor activity, RNA polymerase II-specific (GO:0001227) and the enriched important upregulated pathways were mainly heme metabolism, TGF-beta signaling and spermatogenesis. As for EJ28Pi, 55 DEGs were upregulated (log2 fold-change ≥ 0) and 79 DEGs were downregulated (log2 fold-change ≤ 0). These DEGs resulted in significantly enriched molecular function such as protein domain specific binding (GO:0019904) and RNA polymerase II regulatory region sequence-specific DNA binding (GO:0000977). The enriched important upregulated pathways were allograft rejection, KRAS signaling up and interferon gamma response. Other important pathways that were downregulated in both the NDV-persistently infected cell lines were angiogenesis, apoptosis, and xenobiotic metabolism. Conclusion The transcriptome profiles (RNA-Seq) of these cell lines suggest that evasion of apoptosis and increase in TGF-beta signaling and interferon gamma response activities are crucial for establishment of NDV persistent infection in bladder cancer cells. Findings from this study provide the molecular basis that warrant further study on how bladder cancer cells acquired NDV persistent infection. Resolving the mechanism of persistent infection will facilitate the application of NDV for more effective treatment of bladder cancer.

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High expression of heat shock protein 105 predicts a favorable prognosis for patients with urinary bladder cancer treated with radical cystectomy

Cited by 10 publications

References 25 publications

Expression of Heat Shock Protein 105 in Cutaneous Squamous Cell Carcinoma: Correlation with Clinicopathological Characteristics

Expression of Heat Shock Protein 105 in Cutaneous Squamous Cell Carcinoma: Correlation with Clinicopathological Characteristics

HSP105 expression in cutaneous malignant melanoma: Correlation with clinicopathological characteristics

Functional Transcriptome Analysis of Bladder Cancer Cell Lines Persistently Infected with Oncolytic Newcastle Disease Virus

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