High expression of CD47 in triple negative breast cancer is associated with epithelial‑mesenchymal transition and poor prognosis

Yuan, Jingping; Shi, Xi; Chen, Chuang; He, Huihua; Liu, Lin; Wu, Juan; Yan, Honglin

doi:10.3892/ol.2019.10618

Cited by 54 publications

(70 citation statements)

References 39 publications

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“…As for ZC3H18 (Zinc finger CCCH domain-containing protein 18), our study suggested that the gene of ZC3H18 could be regarded as a protective factor and negatively associated with CD47 ( Figure 9C). Massive studies have shown that overexpression of CD47 predicts poor prognosis and promotes cancer cell invasion in many types of cancer [32][33][34]. What is more, in this correlation network, we found most of alternative splicing events were associated with poor overall survival and positively co-related to splicing factors.…”

Section: Discussionsupporting

confidence: 56%

Alternative Splicing Events as Indicators for the Prognosis of Uveal Melanoma

Wan

Sang

Jin

et al. 2020

Genes

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Growing evidence has revealed that abnormal alternative splicing (AS) events are closely related to carcinogenic processes. However, the comprehensive study on the prognostic value of splicing events involved in uveal melanoma (UM) is still lacking. Therefore, splicing data of 80 UM patients were obtained from the Cancer Genome Atlas (TCGA) SpliceSeq and RNA sequence data of UM and patient clinical features were downloaded from the Cancer Genome Atlas (TCGA) database to identify survival related splicing events in UM. As a result, a total of 37996 AS events of 17911 genes in UM were detected, among which 5299 AS events of 3529 genes were significantly associated with UM patients’ survival. Functional enrichment analysis revealed that this survival related splicing genes are corelated with mRNA catabolic process and ribosome pathway. Based on survival related splicing events, seven types of prognostic markers and the final overall prognostic signature could independently predict the overall survival of UM patients. Finally, an 11 spliced gene was identified in the final signature. On the basis of these 11 genes, we constructed a Support Vector Machine (SVM) classifier and evaluated it with leave-one-out cross-validation. The results showed that the 11 genes could determine short- and long-term survival with a predicted accuracy of 97.5%. Besides, the splicing factors and alternative splicing events correlation network was constructed to serve as therapeutic targets for UM treatment. Thus, our study depicts a comprehensive landscape of alternative splicing events in the prognosis of UM. The correlation network and associated pathways would provide additional potential targets for therapy and prognosis.

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Section: Discussionsupporting

confidence: 56%

Alternative Splicing Events as Indicators for the Prognosis of Uveal Melanoma

Wan

Sang

Jin

et al. 2020

Genes

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“…14 However, recent studies revealed that CD47, which was associated with several clinicopathological characteristics, was not an independent predictor of poor DFS in breast cancer. 10,11 The inconsistency across different studies may be due to variations in immunohistochemical techniques and differences between the cancers, including type, stage, and treatment history. We consider that CD47 is a feasible prognostic biomarker of NPC patients.…”

Section: Discussionmentioning

confidence: 99%

“…9 Several studies have demonstrated that high CD47 expression predicts poor prognosis in several cancers. [10][11][12][13][14] However, little is known about the expression and role of CD47 in NPC.…”

Section: Introductionmentioning

confidence: 99%

<p>CD47 Overexpression Is Associated with Epstein–Barr Virus Infection and Poor Prognosis in Patients with Nasopharyngeal Carcinoma</p>

Wang¹,

Pei²,

Zhu³

et al. 2020

OTT

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Little is known about the clinical significance of CD47 expression and its association with Epstein-Barr virus (EBV) infection in patients with nasopharyngeal carcinoma (NPC). The aim of this study was to clarify the prognostic value and role of CD47 in EBV-associated NPC. Materials and Methods: Sixty-six cases of non-metastatic NPC were retrospectively reviewed. Tissues were collected for immunohistochemical staining of CD47 and the EBVencoded oncoprotein latent membrane protein 1 (LMP1). Western blotting and quantitative real-time PCR were performed to determine the CD47 and LMP1 levels in common human NPC cell lines. Additionally, CD47 and LMP1 expression in a constructed EBV-positive human NPC cell (CNE-2-EBV+) and a stable cell line transfected with LMP1 plasmid (CNE-2-LMP1) was assessed. Next, we used Western blotting to assess the decrease in CD47 expression on CNE-2-LMP1 cells after transfecting them with small interfering RNA (siRNA)-targeting LMP1. Results: In NPC patients, CD47 overexpression was significantly associated with disease recurrence (P=0.010), leading to poorer disease-free survival (DFS; P=0.002) and overall survival (P=0.021). Multivariate Cox proportional hazards models demonstrated that CD47 (HR=5.452, P=0.016) was an independent prognostic factor of DFS. Moreover, CD47 expression was associated with plasma EBV-DNA copy number and LMP1 tissue expression. Among the human NPC cell lines, CD47 and LMP1 expression was notably higher in the EBV-positive C666-1 cell line than in the EBV-negative cell lines. Furthermore, EBV infection upregulated CD47 expression via LMP1-mediated pathways in human NPC cells. Conclusion: This study indicated that CD47 is related to EBV infection in NPC patients, and it is a feasible biomarker.

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“…By binding to signal regulatory protein α (SIRPα), among others expressed by neutrophils, it inhibits phagocytic uptake or trogocytosis ( Figure 4 B; panel 1). Cancer cells hijack this inhibitory CD47–SIRPα pathway by overexpressing CD47, which is associated with poor prognosis in both solid [ 184 , 185 ] and hematological cancers [ 3 , 186 ]. Furthermore, significantly lower CD47 expression levels were detected on breast cancer cells of human patients that responded with a complete response after trastuzumab plus vinorelbine therapy [ 187 ].…”

Section: Fcr-mediated Neutrophil Activation; Targeting Fcγriia (Cdmentioning

confidence: 99%

The Neutrophil: The Underdog That Packs a Punch in the Fight against Cancer

Avtenyuk

Visser

Bremer

et al. 2020

IJMS

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The advent of immunotherapy has had a major impact on the outcome and overall survival in many types of cancer. Current immunotherapeutic strategies typically aim to (re)activate anticancer T cell immunity, although the targeting of macrophage-mediated anticancer innate immunity has also emerged in recent years. Neutrophils, although comprising ≈ 60% of all white blood cells in the circulation, are still largely overlooked in this respect. Nevertheless, neutrophils have evident anticancer activity and can induce phagocytosis, trogocytosis, as well as the direct cytotoxic elimination of cancer cells. Furthermore, therapeutic tumor-targeting monoclonal antibodies trigger anticancer immune responses through all innate Fc-receptor expressing cells, including neutrophils. Indeed, the depletion of neutrophils strongly reduced the efficacy of monoclonal antibody treatment and increased tumor progression in various preclinical studies. In addition, the infusion of neutrophils in murine cancer models reduced tumor progression. However, evidence on the anticancer effects of neutrophils is fragmentary and mostly obtained in in vitro assays or murine models with reports on anticancer neutrophil activity in humans lagging behind. In this review, we aim to give an overview of the available knowledge of anticancer activity by neutrophils. Furthermore, we will describe strategies being explored for the therapeutic activation of anticancer neutrophil activity.

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High expression of CD47 in triple negative breast cancer is associated with epithelial‑mesenchymal transition and poor prognosis

Cited by 54 publications

References 39 publications

Alternative Splicing Events as Indicators for the Prognosis of Uveal Melanoma

Alternative Splicing Events as Indicators for the Prognosis of Uveal Melanoma

<p>CD47 Overexpression Is Associated with Epstein–Barr Virus Infection and Poor Prognosis in Patients with Nasopharyngeal Carcinoma</p>

The Neutrophil: The Underdog That Packs a Punch in the Fight against Cancer

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