High ethanol yields using Aspergillus oryzae koji and corn media

Ammonium acceleration of glycolysis in the ethanol fermentation in a high corn system.

“…This stimulation of glycolysis probably explains the similar high ethanol fermentation rates obtained with A. oryzae koji saccharified corn mashes (4,5), since such corn mashes would undoubtedly contain increased amounts of solublized nitrogen compounds. This view is further supported by the observation that the addition of (NH4)25O4 or NH4C1 to A, oryzae koji saccharified corn mashes did not result in an additional stimulation of the fermentation rate, beyond the high rate already observed (data not shown).…”

“…The preparation of the S. cerevisiae Y-567 inoculum and fermentation procedure was as previously described (4,5), with a final inoculation rate of 2.5 x 106 cells ml. Due to the very dense corn mash solids, it was impossible to sample the fermentation flasks during the run.…”