High-efficiency somatic reprogramming induced by intact MII oocytes

Yang, Hui; Shi, Linqi; Zhang, Shenghua; Ling, Jiangwei; Jiang, Jing; Li, Jinsong

doi:10.1038/cr.2010.97

Cited by 17 publications

(14 citation statements)

References 32 publications

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“…Mouse tetraploid embryos were successfully generated by fusing two blastomeres within the zona pellucida and can also be produced by injecting somatic cells into intact MII oocytes 7 . A previous study of tetraploid mouse embryo development showed that tetraploid embryos can undergo compaction and form cavities similar to diploid embryos 8 ; furthermore, tetraploid mouse embryos could be recovered on the 15th day of gestation, but their development was retarded 9 .…”

Section: Introductionmentioning

confidence: 99%

Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues

Wen

Cheng

et al. 2017

Sci Rep

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Our study examined the in vivo chimeric and survival capacities of chimeras created by injecting tetraploid embryonic stem cells (ESCs) expressing green fluorescent protein (GFP) into diploid embryos. At 3.5 days post-coitum (dpc) and 4.5 dpc, the tetraploid ESCs were able to contribute to the inner cell mass (ICM) just as diploid ESCs tagged with GFP. At 6.5 dpc, 8.0 dpc and 10.5 dpc, the tetraploid ESCs manifested in the same location as the diploid ESCs. The GFP cells in the extraembryonic tissues and fetuses of tetraploid ESC chimeras were tetraploid as determined by fluorescence activated cell sorting (FACS). Furthermore, tetraploid ESCs contributed to the development of the placenta, embryolemma and umbilical cord at 13.5 dpc and 16.5 dpc; however, very less GFP cells were found in the fetuses of tetraploid ESC chimeras. We further found that the proliferation of tetraploid ESCs was slower than that of diploid ESCs. In addition, the relative mRNA expression in the three germ layers and the trophoblast was abnormal in the EBs of tetraploid ESCs compared with diploid ESCs. In short, slower proliferation and abnormal differentiation potential of tetraploid ESCs might be two of the reasons for their poor survival and chimeric capacities.

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Section: Introductionmentioning

confidence: 99%

Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues

Wen

Cheng

et al. 2017

Sci Rep

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“…Recently, an intriguing study showed a dramatic improvement in the efficiency of ESC line derivation following SCNT 63 . Instead of using enucleated oocytes, the authors injected somatic cells into intact MII oocytes and confirmed the reprogramming capability of these cells.…”

Section: Somatic Cell Nuclear Transfermentioning

confidence: 98%

“…Recently, an intriguing study showed a dramatic improvement in the efficiency of ESC line derivation following SCNT. 63 Instead of using enucleated oocytes, the authors injected somatic cells into intact MII oocytes and confirmed the reprogramming capability of these cells. The reported efficiency of ESCs generation per injected oocyte was 30%, and the pluripotent ESCs were shown to have undergone epigenetic reprogramming at the Oct4 promoter.…”

Section: Escs From Primates By Means Of Scnt Encourages Further Consimentioning

confidence: 99%

Germ cell formation from embryonic stem cells and the use of somatic cell nuclei in oocytes

Pelosi

Forabosco²,

Schlessinger

2011

Annals of the New York Academy of Sciences

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Embryonic stem cells (ESCs) have remarkable properties of pluripotency and self-renewal, along with the retention of chromosomal integrity. Germ cells function as a kind of “transgenerational stem cells”, transmitting genetic information from one generation to the next. The formation of putative primordial germ cells (PGCs) and germ cells from mouse and human ESCs has, in fact, been shown, and the apparent derivation of functional mouse male gametes has also been described. Additionally investigators have successfully reprogrammed somatic nuclei into a pluripotent state by inserting them into ESCs or oocytes. This would enable the generation of ESCs genetically identical to the somatic cell donor and their use in cell therapy. However, these methodologies are still inefficient and their mechanisms poorly understood. Until full comprehension of these processes is obtained, clinical applications remain remote. Nevertheless, they represent promising tools in the future, enhancing methods of therapeutic cloning and infertility treatment.

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“…In the presence of oocyte nuclei, the transferred cell nuclei underwent NEBD and entered pre-metaphase I stage 5 times faster than the enucleated group. This phenomenon was also observed in mice by using an intact oocyte as the recipient cytoplast [63]. To verify oocyte nucleus capability for cell reprogramming, a reverse nuclear transfer (RNT) procedure was performed.…”

Section: Second Metaphase Stage Cytoplasts As Recipientsmentioning

confidence: 99%

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

Yin¹,

Liu²,

Bou³

et al. 2014

Front. Agr. Sci. Eng.

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Oocytes are unique cells with the inherent capability to reprogram nuclei. The reprogramming of the somatic nucleus from its original cellular state to a totipotent state is essential for term development after somatic cell nuclear transfer. The nuclear-associated factors contained within oocytes are critical for normal fertilization by sperm or for somatic cell nuclear reprogramming. The chromatin of somatic nuclei can be reprogrammed by factors in the egg cytoplasm whose natural function is to reprogram sperm chromatin. The oocyte first obtains its reprogramming capability in the early fetal follicle, and then its capacity is enriched in the late growth phase and reaches its highest capability for reprogramming as fully-grown germinal vesicle oocytes. The cytoplasmic milieu most likely contains all of the specific transcription and/or reprogramming factors necessary for cellular reprogramming. Certain transcription factors in the cytoplast may be critical as has been demonstrated for induced pluripotent stem cells. The maternal pronucleus exerts a predominant, transcriptiondependent effect on embryo cytofragmentation, with a lesser effect imposed by the ooplasm and the paternal pronucleus. With deep analysis of transcriptomics in oocytes and early developmental stage embryos more maternal transcription factors inducing cellular reprogramming will be identified.

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High-efficiency somatic reprogramming induced by intact MII oocytes

Cited by 17 publications

References 32 publications

Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues

Tetraploid embryonic stem cells can contribute to the development of chimeric fetuses and chimeric extraembryonic tissues

Germ cell formation from embryonic stem cells and the use of somatic cell nuclei in oocytes

Oocyte-associated transcription factors in reprogramming after somatic cell nuclear transfer: a review

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