High efficiency reporter gene transfection of vascular tissue in vitro and in vivo using a cationic lipid–DNA complex

Transfection of cultured myoblasts in high serum concentration with DODAC:DOPE liposomes

“…Several studies have reported the optimization of cationic-liposome-DNA complexes in transfecting several tissues (eg lungs, arteries, bone marrow) both in vitro and in vivo, 10,16,17 but no in vivo study has focused on skeletal muscle. The few reports that have dealt with the use of cationic liposomes to transfect cultured muscle cells obtained significant transfection levels only in a serumfree environment.…”

“…To date, all liposome formulations reported have transfection efficiencies that are severely affected by serum in the medium and consequently transfection of cultured cells with cationic liposomes has been carried out in serum-free conditions or at most in 10-15% serum. [8][9][10][11][12] A liposome formulation with a transfection efficiency that is not inhibited by serum in the extracellular environment would provide a considerable advance toward the goal of systemic delivery.…”

Efficient adventitial gene delivery to rabbit carotid artery with cationic polymer–plasmid complexes

“…[1][2][3][4][5][6][7][8][9][10][11] To date, viral systems have been more efficient than nonviral vehicles in vascular gene transfer. 10,12,13 However, the use of viral vectors in gene therapy has raised questions concerning their immunogenicity, oncogenic properties and unknown long-term effects.…”

Tissue-selective expression of dominant-negative proteins for the regulation of vascular smooth muscle cell proliferation

“…Following transfection into mammalian cells it is expected that the pCI vector will support expression of genes linked in cis in all cell types, while the genes will be expressed only in muscle cells when the pCI/h999 vector is used. 39 To confirm this expression pattern, the MycEn and MybEn constructs in the pCI or pCI/h999 vectors were transfected into rabbit VSMCs or the HUVEC cell line ECV304 as described 39,40 and total RNA harvested 36 h later for Northern analysis. The resulting membranes were probed for engrailed or the endogenous ribosomal pro- …”