2023
DOI: 10.1038/s42003-023-04859-2
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High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications

Abstract: Extracellular vesicles (EVs) are highly interesting for the design of next-generation therapeutics. However, their preparation methods face challenges in standardization, yield, and reproducibility. Here, we describe a highly efficient and reproducible EV preparation method for monodisperse nano plasma membrane vesicles (nPMVs), which yields 10 to 100 times more particles per cell and hour than conventional EV preparation methods. nPMVs are produced by homogenizing giant plasma membrane vesicles following cell… Show more

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Cited by 4 publications
(4 citation statements)
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“…43 This protocol resulted in blebs with cell-like mechanical properties 44 that were then extruded to produce 200nm-diameter ‘large’ plasma membrane vesicles (LPMVs). 45…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…43 This protocol resulted in blebs with cell-like mechanical properties 44 that were then extruded to produce 200nm-diameter ‘large’ plasma membrane vesicles (LPMVs). 45…”
Section: Resultsmentioning
confidence: 99%
“…43 This protocol resulted in blebs with cell-like mechanical properties 44 that were then extruded to produce 200nm-diameter 'large' plasma membrane vesicles (LPMVs). 45 Figure 1, Schematic diagram of experimental approach to produce EVs and LPMVs. EVs are collected from the supernatant of cultured MDA-MB-231 cells and are purified with SEC (upper path).…”
Section: Evs and Plasma Membrane Vesicles: Collection Synthesis And C...mentioning
confidence: 99%
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“…EVs were once thought to function as cellular garbage disposals [ 2 ], but it is now accepted that EVs play an important role in cellular communication [ 3 , 4 , 5 ], intracellular signaling [ 6 , 7 , 8 ], and cellular differentiation [ 9 , 10 , 11 ]. In addition, EVs are used in multiple applications [ 12 , 13 , 14 ]. EVs isolated from biological fluids including urine [ 15 ], plasma [ 16 ], and cerebrospinal fluid [ 17 ] represent a rich source of biomarkers.…”
Section: Introductionmentioning
confidence: 99%