High-Efficiency Loading and Controlled Release of Doxorubicin Hydrochloride on Graphene Oxide

Yang, Xiaoying; Zhang, Xiaoyan; Liu, Zunfeng; Ma, Yanfeng; Huang, Yi; Chen, Yongsheng

doi:10.1021/jp806751k

Cited by 920 publications

(641 citation statements)

References 23 publications

(26 reference statements)

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“…37,38 On the other hand, it means that appropriate mechanism may be pursued by further work to trigger the effective release in tumor tissues for the desirable chemotherapy performance. 57 Cell Uptake of GO/NP/DOX Complex. Efficient cellular uptake of drug payloads is one of the crucial properties for nanotherapeutics for meaningful drug efficacy.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

et al. 2013

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In this paper, a facile strategy to develop graphenebased delivery nanosystems for effective drug loading and sustained drug release was proposed and validated. Specifically, biocompatible naphthalene-terminated PEG (NP) and anticancer drugs (curcumin or doxorubicin (DOX)) were simultaneously integrated onto oxidized graphene (GO), leading to selfassembled, nanosized complexes. It was found that the oxidation degree of GO had a significant impact on the drug-loading efficiency and the structural stability of nanosystems. Interestingly, the nanoassemblies resulted in more effective cellular entry of DOX in comparison with free DOX or DOX-loaded PEGpolyester micelles at equivalent DOX dose, as demonstrated by confocal microscopy studies. Moreover, the nanoassemblies not only exhibited a sustained drug release pattern without an initial burst release, but also significantly improved the stability of formulations which were resistant to drug leaking even in the presence of strong surfactants such as aromatic sodium benzenesulfonate (SBen) and aliphatic sodium dodecylsulfonate (SDS). In addition, the nanoassemblies without DOX loading showed negligible in vitro cytotoxicity, whereas DOX-loaded counterparts led to considerable toxicity against HeLa cells. The DOX-mediated cytotoxicity of the graphene-based formulation was around 20 folds lower than that of free DOX, most likely due to the slow DOX release from complexes. A zebrafish model was established to assess the in vivo safety profile of curcumin-loaded nanosystems. The results showed they were able to excrete from the zebrafish body rapidly and had nearly no influence on the zebrafish upgrowth. Those encouraging results may prompt the advance of graphene-based nanotherapeutics for biomedical applications.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

et al. 2013

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“…This difference was surmised to be due to a more favorable π-stacking of doxorubicin onto larger diameter CNTs because they have flatter sidewalls. In comparison, doxorubicin is retained much more readily on graphene because of its flat structure [40].…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors

Stefansson¹,

Stefansson²,

Chung³

et al. 2012

Journal of Nanotechnology

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Biomolecular detections performed on carbon nanotube field-effect transistors (CNT-FETs) frequently use reactive pyrenes as an anchor to tether bioactive ligands to the hydrophobic nanotubes. In this paper, we explore the possibility of directly using bioactive aromatic compounds themselves as CNT-FET ligands. This would be an efficient way to functionalize CNT-FETs since many aromatic compounds bind avidly to nanotubes, and it would also ensure that ligand-binding molecules would be brought in close proximity to the nanotubes. Using a model system consisting of pyrene, phenanthrene, naphthalene, or phenyl boronic acids immobilized on CNT-FET wafers, we show that all are able to bind glycated human serum albumin (gHSA), which is an important diabetes marker. Pyrene boronic acid proved to bind CNTs with the greatest apparent affinity as measured by gHSA impedance. Interestingly, gHSA CNT-FET signal intensity, which is proportional to amount of protein bound, remained essentially unchanged for all the boronic acids tested.

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“…Anthracyclines, especially DNR and DOX have been widely investigated using various spectroscopic methods, e.g. Raman spectroscopy [24,25], Resonance Raman (RR) [26][27][28], Surface Enhanced Raman Spectroscopy (SERS) [29][30][31][32], UV-Vis absorption [26,33] and FT-IR absorption spectroscopy [24,34,35]. The visible absorption spectra of DOX and DNR are, however, almost identical, having a maximum of absorption near to 480-490 nm.…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic studies of anthracyclines: Structural characterization and in vitro tracking

Szafraniec

Majzner

Farhane³

et al. 2016

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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A broad spectroscopic characterization, using ultraviolet-visible (UV-vis) and Fourier transform infrared absorption as well as Raman scattering, of two commonly used anthracyclines antibiotics (DOX) daunorubicin (DNR), their epimers (EDOX, EDNR) and ten selected analogs is presented. The paper serves as a comprehensive spectral library of UV-vis, IR and Raman spectra of anthracyclines in the solid state and in solution. The particular advantage of Raman spectroscopy for the measurement and analysis of individual antibiotics is demonstrated. Raman spectroscopy can be used to monitor the in vitro uptake and distribution of the drug in cells, using both 488 nm and 785 nm as source wavelengths, with submicrometer spatial resolution, although the cellular accumulation of the drug is different in each case. The high information content of Raman spectra allows studies of the drug-cell interactions, and so the method seems very suitable for monitoring drug uptake and mechanisms of interaction with cellular compartments at the subcellular level.

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High-Efficiency Loading and Controlled Release of Doxorubicin Hydrochloride on Graphene Oxide

Cited by 920 publications

References 23 publications

Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

Evaluation of Aromatic Boronic Acids as Ligands for Measuring Diabetes Markers on Carbon Nanotube Field-Effect Transistors

Spectroscopic studies of anthracyclines: Structural characterization and in vitro tracking

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