High efficiency gene transfer to the central nervous system of rodents and primates using herpes virus vectors lacking functional ICP27 and ICP34.5

Mk, Howard; Kershaw, T. R.; Gibb, Barry; Storey, Nina M.; Ar, MacLean; By, Zeng; Bc, Tel; Jenner, Peter; Sm, Brown; Cj, Woolf; Anderson, PN; Rs, Coffin; Ds, Latchman

doi:10.1038/sj.gt.3300700

Cited by 50 publications

(41 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[7][8][9] Common marmosets are bred in designated Primate Research Facilities within the United States and as such these animals are readily available at most biomedical research centers, thus facilitating experimental planning and potentially accelerating transitions into important clinical trials.…”

Section: Introductionmentioning

confidence: 99%

Development of a novel non-human primate model for preclinical gene vector safety studies. Determining the effects of intracerebral HSV-1 inoculation in the common marmoset: a comparative study

et al. 2003

View full text Add to dashboard Cite

The owl monkey (Aotus trivirgatus) has served as the standard non-human primate model of herpes simplex virus-1 (HSV-1) infection because it is highly susceptible to HSV-1 encephalitis. Owl monkeys, however, are expensive, difficult to obtain, and difficult to maintain in captivity, thus greatly hampering the efficiency of preclinical gene therapy trials for brain tumors using HSV-1-based vectors. We have therefore compared the susceptibility of the common marmoset (Callithrix jacchus) with the owl monkey in a model of intracerebral inoculation of wildtype HSV-1 F-strain at increasing titers. The common marmosets consistently succumbed earlier to viral encephalitis than the owl monkeys.The histological evaluation of the common marmoset revealed extensive HSV-1 infection with a concomitant yet less marked inflammatory response compared to the owl monkeys. PCR for HSV-1 demonstrated a similar extra-CNS shedding route in both experimental models. Our findings show that the common marmoset is at least as susceptible to intracerebral HSV-infection as the owl monkey and that it can therefore serve as a valid and reliable experimental model for the important preclinical safety tests of HSV-based therapeutic viral vector constructs in the brain.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of a novel non-human primate model for preclinical gene vector safety studies. Determining the effects of intracerebral HSV-1 inoculation in the common marmoset: a comparative study

et al. 2003

View full text Add to dashboard Cite

show abstract

“…The newly synthesised viral particles are released from the infected cells by lysis. Since many of the HSV proteins are nonessential for viral replication, they can be removed and replaced with the target therapeutic genes [68,105].…”

Section: Non-integrating Viral Vectorsmentioning

confidence: 99%

RNA Interference with Special Reference to Combating Viruses of Crustacea

Fauce

Owens

2012

Indian J. Virol.

View full text Add to dashboard Cite

RNA interference has evolved from being a nuisance biological phenomenon to a valuable research tool to determine gene function and as a therapeutic agent. Since pioneering observations regarding RNA interference were first reported in the 1990s from the nematode worm, plants and Drosophila, the RNAi phenomenon has since been reported in all eukaryotic organisms investigated from protozoans, plants, arthropods, fish and mammals. The design of RNAi therapeutics has progressed rapidly to designing dsRNA that can specifically and effectively silence disease related genes. Such technology has demonstrated the effective use of short interfering as therapeutics. In the absence of a B cell lineage in arthropods, and hence no long term vaccination strategy being available, the introduction of using RNA interference in crustacea may serve as an effective control and preventative measure for viral diseases for application in aquaculture.

show abstract

“…Each virus was plaque purified following homologous recombination of the respective plasmids into ICP27-deleted virus DNA. 11 The photomicrographs in Figure 2 clearly demonstrate that in B130/2 cells infected with the 17+pR19 IRES virus (Figure 2a and b) where the deletion of ICP27 is complemented, both reporter genes are expressed to a high level. However, it is also clear that the expression of both the transgene upstream and downstream of the EMCV IRES is decreased in comparison to B130/2 cells infected with the single transgene-expressing 17+pR19 lacZ and 17+pR19 GFP viruses (Figure 2c and d).…”

Section: Figure 2 Reporter Gene Expression In B130/2 Cells Infected Wmentioning

confidence: 99%

“…10,11 Therefore, the vectors used for this study were also deleted for ICP27. Transgene expression was driven by the human cytomegalovirus immediate-early (CMV IE) promoter and the promoter/transgene cassette was inserted by homologous recombination into the 2 kb LAT sequence in the HSV-1 genome (strain 17+).…”

Section: Figure 1 Schematic Representation Of the 17+pr19-based Hsv-1mentioning

confidence: 99%

Gene transfer using a disabled herpes virus vector containing the EMCV IRES allows multiple gene expression in vitro and in vivo

et al. 1998

Self Cite

View full text Add to dashboard Cite

High efficiency gene transfer to the central nervous system of rodents and primates using herpes virus vectors lacking functional ICP27 and ICP34.5

Cited by 50 publications

References 22 publications

Development of a novel non-human primate model for preclinical gene vector safety studies. Determining the effects of intracerebral HSV-1 inoculation in the common marmoset: a comparative study

Development of a novel non-human primate model for preclinical gene vector safety studies. Determining the effects of intracerebral HSV-1 inoculation in the common marmoset: a comparative study

RNA Interference with Special Reference to Combating Viruses of Crustacea

Gene transfer using a disabled herpes virus vector containing the EMCV IRES allows multiple gene expression in vitro and in vivo

Contact Info

Product

Resources

About