High-efficiency endovascular gene delivery via therapeutic ultrasound

Amabile, Philippe; Waugh, Jacob M.; Lewis, Thomas N.; Elkins, Christopher J.; Janas, W.; Dake, Michael D.

doi:10.1016/s0735-1097(01)01253-0

Cited by 67 publications

(40 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Secondly, the sonication needed to achieve enhanced gene expression by either plasmid alone or coupled to MSP is within the range emitted by diagnostic transducers. [107][108][109] Thus, if efficient gene transfer could be achieved by this technique, it would have great advantages in clinical use over other physical methods, such as electroporation. Thirdly, enhanced transgene expression can be achieved by US alone or in combination with other non-viral delivery systems, such as lipoplex-mediated gene delivery.…”

Section: Microbubbles and Ultrasoundmentioning

confidence: 99%

Non-viral gene delivery in skeletal muscle: a protein factory

Lu¹,

Bou‐Gharios²,

Partridge³

2003

Gene Ther

169

109

View full text Add to dashboard Cite

Ever since the publication of the first reports in 1990 using skeletal muscle as a direct target for expressing foreign transgenes, an avalanche of papers has identified a variety of proteins that can be synthesized and correctly processed by skeletal muscle. The impetus to the development of such applications is not only amelioration of muscle diseases, but also a range of therapeutic applications, from immunization to delivery of therapeutic proteins, such as clotting factors and hormones. Although the most efficient way of introducing transgenes into muscle fibres has been by a variety of recombinant viral vectors, there are potential benefits in the use of non-viral vectors. In this review we assess the recent advances in construction and delivery of naked plasmid DNA to skeletal muscle and highlight the options available for further improvements to raise efficiency to therapeutic levels.

show abstract

Section: Microbubbles and Ultrasoundmentioning

confidence: 99%

Non-viral gene delivery in skeletal muscle: a protein factory

Lu¹,

Bou‐Gharios²,

Partridge³

2003

Gene Ther

169

109

View full text Add to dashboard Cite

show abstract

“…Indeed, enhancement of gene expression was observed by irradiating ultrasonic wave to the tissue after injection of DNA. 20,21 Since ultrasound application is flexible and safe, its use in gene delivery has a great advantage in clinical use. Recently, it was reported that combination of microbubble with ultrasound could further increase the gene expression level.…”

Section: Introductionmentioning

confidence: 99%

Gene Therapy Progress and Prospects: Nonviral vectors

2002

View full text Add to dashboard Cite

“…Since the sonication power needed for achieving enhanced gene expression is within safe therapeutic and imaging limits, 13,16,17 this method might be combined with ultrasound imaging of a target tissue. This would give it an advantage over other methods, such as electroporation, for which clinically safe limits have yet to be established.…”

Section: Introductionmentioning

confidence: 99%