High-efficiency Agrobacterium rhizogenes-mediated genetic transformation in Artemisia vulgaris: Hairy root production and essential oil analysis

Sujatha, G.; Zdravković-Korać, Snežana; Ćalić﻿﻿﻿, Dušica; Flamini, Guido; Kumari, B. D. Ranjitha

doi:10.1016/j.indcrop.2012.09.007

Cited by 55 publications

(35 citation statements)

References 28 publications

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“…In our experiments, the maximum GI, which was obtained in 1/2 MS-B5 treatment, was 1.8-fold higher than that obtained in the MS treatment. These results are consistent with those of Sujatha et al (2013) and suggest that a low level of an inorganic nitrogen source in the medium (1/2 MS) and a high level of an organic nitrogen source (B5 vitamins) were more suitable for hairy root survival and growth. MS medium is very rich, and saline medium may be too salty for HR growth and sometimes, due to lower concentration of thiamine present, the vitamins can be replaced with B5 vitamins.…”

Section: Discussionsupporting

confidence: 89%

Establishment of Persicaria minor hairy roots and analysis of secreted β-caryophyllene in medium broth

Ashraf

Zain

Zainal

et al. 2014

Plant Cell Tiss Organ Cult

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A method for hairy root induction of Persicaria minor, was developed and investigated, and a qualitative evaluation of the sesquiterpenes in the culture medium was performed. The transgenic status was confirmed by PCR analysis using rolB and virD specific primers. The efficiency with which four different strains of Agrobacterium rhizogenes (A4, ATCC43056, ATCC15834 and ATCC13333) and different concentrations of acetosyringone induced hairy roots from in vitro leaf segments of P. minor was evaluated. Strains A4 and ATCC13333 were able to induce hairy root in an average of 6 days of cocultivation and exhibited significantly higher transformation efficiencies, 20 and 21.3 %, respectively, compared to the other strains in the presence of 100 lM acetosyringone. Half-strength Murashige-Skoog basal medium supplemented with Gamborg vitamins supported the greatest increase (35-fold) of growth index of hairy roots relative to the initial weight after 8 weeks of culture. GC-MS results showed that b-caryophyllene was the main sesquiterpenes detected and was quantified with standards, with total peak areas of 7.934. Results suggested that hairy roots secreted sesquiterpenes into the medium while preserving viable hairy roots as a source of specialized metabolites.

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Section: Discussionsupporting

confidence: 89%

Establishment of Persicaria minor hairy roots and analysis of secreted β-caryophyllene in medium broth

Ashraf

Zain

Zainal

et al. 2014

Plant Cell Tiss Organ Cult

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“…Such roots have been established from many important medicinal plants for the production of active principle (Sujatha et al 2013;Tiwari et al 2007;Giri et al 2001) and these roots have also played important role in the conservation of these plant species (Tiwari et al 2007;Thimmaraju et al 2008). Root induction potential of the various strains of A. rhizogenes varied significantly (Table 1) and maximum root induction was observed in case of strain SA79 followed by strain R1000.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, screening of established lines of hairy roots would be important for the selection of a promising line (Sujatha et al 2013;Giri et al 2001). Not many reports are available on A. rhizogenes-mediated hairy root establishment of B. monnieri except one preliminary study (Majumdar et al 2011), wherein 'bacoside A' production from shoot buds and callus derived from hairy roots was reported.…”

Section: Introductionmentioning

confidence: 99%

Influence of Agrobacterium rhizogenes strains on hairy root induction and ‘bacoside A’ production from Bacopa monnieri (L.) Wettst.

2014

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Stable lines of hairy roots were established from leaf explants of Bacopa monnieri using different strains (A4, R1000, SA79, MTCC 532 and MTCC 2364) of Agrobacterium rhizogenes. The efficiency of hairy roots induction of these strains varied significantly and the maximum transformation frequency (75 %) was observed in case of strain SA79 using leaf explants followed by internode (55 %) in the presence of acetosyringone. Different parameters such as cell density of Agrobacterium suspension, co-cultivation period and infection time influenced the root induction frequency. Maximum frequency of root induction was obtained with bacterial density of 0.6 OD 600 , 2 days of co-cultivation period and 10 min of infection time. Integration of T-DNA in the genome of hairy roots was confirmed by PCR amplification of rolB gene. Elimination of Agrobacterium from the established root cultures was ascertained by amplifying the DNA fragment specific to 16S rDNA and virD gene. All lines of hairy roots except strain A4 induced showed higher growth rate and accumulated higher levels of 'bacoside A' than the untransformed roots. Maximum biomass accumulation (6.8 g l -1 ) and 'bacoside A' content (10.02 mg g -1 DW) were recorded in case of the hairy root line induced by strain MTCC 2364.

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“…Depending upon the target tissue, the transformation frequency varies among plant species, and hence, the identification of suitable explant is indispensible for successful transformation. Root explants superseded other explants in Taraxacum platycarpum (Lee et al, 2004) while explants such as petiole in W. somnifera (Saravanakumar et al, 2012), hypocotyls in Psoralea corylifolia L. (Abhyankar et al, 2005), leaf in Artemisia vulgaris (Sujatha et al, 2013) and stem in Gentiana scabra (Huang et al, 2014) were found to be highly susceptible to A. rhizogenes strains.…”

Section: Factors Influencing Transformation Frequencymentioning

confidence: 99%

Methyl jasmonate elicits the solasodine production and anti-oxidant activity in hairy root cultures of Solanum trilobatum L.

Sakthivel

Satish

Kavikkuil

et al. 2015

Industrial Crops and Products

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High-efficiency Agrobacterium rhizogenes-mediated genetic transformation in Artemisia vulgaris: Hairy root production and essential oil analysis

Cited by 55 publications

References 28 publications

Establishment of Persicaria minor hairy roots and analysis of secreted β-caryophyllene in medium broth

Establishment of Persicaria minor hairy roots and analysis of secreted β-caryophyllene in medium broth

Influence of Agrobacterium rhizogenes strains on hairy root induction and ‘bacoside A’ production from Bacopa monnieri (L.) Wettst.

Methyl jasmonate elicits the solasodine production and anti-oxidant activity in hairy root cultures of Solanum trilobatum L.

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