High Drug Loading Nanoparticles Stabilized with Autologous Serum Proteins Passively Inhibits Tumor Growth

Abstract

We report drug nanocrystals stabilized with host-specific serum proteins with high loading (∼63% w/w). The human serum derived curcumin nanoparticles (Cur-NanoSera) showed superior in vitro anticancer efficiency compared to a free drug with substantial hemocompatibility. The preadsorbed protein coating impeded further protein corona formation, even with repeated serum exposures. Acute and subacute toxicity evaluations post single and dual injections of C57BL/6 mice indicated that Cur-NanoSera showed no prominent inflammatory response or organ damage in the in-bred mice. Passive accumulation of Cur-NanoSera in tumor tissue significantly suppressed its growth in a syngeneic breast tumor model in addition to controlling tumor burden associated splenomegaly.

Plasmonic nanodendrites stabilized with autologous serum proteins for sustainable host specific photothermal tumor ablation

“…26 Our group has recently developed autologous approaches to utilize host derived serum proteins as stabilizers of drug nanocrystals and metal quantum clusters broadly termed 'NanoSera'. 27,28 Considering the established preclinical safety of this approach, in the present work we involved autologous whole serum proteins to form gold nanodendrites, also named Plasmonic-NanoSera (PNS). This rapid and seedless one pot synthesis allows serum proteins to serve as both a template and stabilizer of the nanodendrites.…”

Shielding against breast tumor relapse with an autologous chemo-photo-immune active Nano–Micro-Sera based fibrin implant

“…18 Recently our group showed that API nanocrystals stabilized with autologous serum proteins with high drug loading (>60% w/w) termed 'NanoSera'. 19 Furthermore, we have demonstrated the stabilization of photo-active metal nanoparticles with autologous serum proteins with confirmed safety in mouse models. 20,21 Building on previous reports, an 'all-in-one' autologous therapeutic strategy has been formulated, emphasizing the predominant utilization of host-derived biomaterials in the development of a hybrid fibrin glue.…”

“…While albumin is a known drug carrier, apolipoproteins provide a stealth effect counterbalancing the opsonization effect of the globulins. 19,20,35,36…”

“…The synthesis process excludes additional surfactants or cross-linkers, involving only minimal components such as the drug and whole serum proteins. 19 Through a straightforward LAS process, which entails mixing serum and DMSO containing lapatinib under optimal conditions, spherical Lap-NS with a diameter of approximately 250 nm and a surface charge of around −10 mV were successfully formed (Fig. 2A, S1 † and 2C).…”

Preclinical safety assessment of photoluminescent metal quantum clusters stabilized with autologous serum proteins for host specific theranostics

“…Prior study utilized host derived serum protein to form nanovesicles for patient specific nanomedicines 36 . Our group recently showed that drug nanocrystals can be directly stabilized with autologously derived serum proteins for cancer therapy 37 . This approach takes advantage of the endogenously derived proteins that are less likely to cause inflammatory response in addition to being eventually metabolized safely by the body.…”