High doses of nandrolone decanoate reduce volume of testis and length of seminiferous tubules in rats

Noorafshan, Ali; Karbalay‐Doust, Saied; Mesbah, Fakhroddin

doi:10.1111/j.1600-0463.2005.apm1130205.x

Cited by 41 publications

(36 citation statements)

References 16 publications

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“…ND was injected in doses of 3 mg/kg of body weight. It was administered in one, two and three mg/kg doses in the first, second, and third week of treatment, respectively [4]. The mice were euthanized by inhalation of ethyl ether 72 hours after the last injection.…”

Section: Methodsmentioning

confidence: 99%

“…The side effects of anabolic steroids on the physiology and function of the reproductive system have been proved [3]. The side effects of steroids on the histology of testis [4], prostate [5], ovary, and uterus [6][7], spermatogenesis [8], genitalia malformation, and ovarian cyclical activity [1,[9][10][11] have also been shown. Ovarian and uterine histology and follicular score have been investigated after the ND treatment [6,7]; however, quantitative aspects of the ovary and oocytes have received little attention.…”

Section: Introductionmentioning

confidence: 99%

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Stereological estimation of ovarian oocyte volume, surface area and number: application on mice treated with nandrolone decanoate

Karbalay‐Doust

Noorafshan

2012

Folia Histochem Cytobiol.

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Changes in the number and size of oocytes can lead to fertilization problems. The present study aimed to evaluate the number, volume, and surface area of oocytes in healthy as well as nandrolone decanoate-treated (ND) mice using stereological methods. Five control mice received vehicle, and five ND-treated mice received ND. Using the 'isotropic Cavalieri' design', the ovary was sectioned. The volume of the ovary (cortex and medulla) was estimated. The oocytes' volume and surface area were estimated using the invariator. The number of the oocytes was estimated using an optical disector. The volumes of the ovary, cortex, and medulla decreased ~50% in the ND-treated mice. The mean number (coefficient of variation) of preantral, antral, and atretic oocytes in the control ovary were 1,690 (0.29), 2,100 (0.52), and 3,900 (0.2), respectively, which decreased ~54%, ~87%, and ~91%, respectively in the ND-treated animals. The mean volume (coefficient of variation) of the preantral, antral, and atretic oocytes were 86,000 (0.27), 110,000 (0.48), and 27,000 (0.33) mm 3 , respectively. The mean surface area (coefficient of variation) of the three types of oocytes were 9,000 (0.24), 9,900 (0.28), and 4,700 (0.21) mm 2 , respectively. These parameters remained unchanged in the ND-treated mice. ND induces reduction in the number of oocytes, but not in the volume or the surface area.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Stereological estimation of ovarian oocyte volume, surface area and number: application on mice treated with nandrolone decanoate

Karbalay‐Doust

Noorafshan

2012

Folia Histochem Cytobiol.

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“…The use of anabolic-androgenic steroids can lead to various body changes, including to the male genital system (12)(13)(14). Many studies have been published reporting structural changes of testes after AAS use (3)(4)(5). High doses of nandrolone decanoate reduce the testis volume and length of seminiferous tubules in rats (4).…”

Section: Discussionmentioning

confidence: 99%

“…Testicular changes resulting from AAS use are well documented in the literature (3). High doses of nandrolone decanoate reduce the volume of the testis and length of seminiferous tubules in rats (4).…”

Section: Introductionmentioning

confidence: 97%

The prostate after administration of anabolic androgenic steroids: a morphometrical study in rats

et al. 2013

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Purpose: Many adverse effects have been associated with abuse of anabolic-androgenic steroids (AAS), including disorders of the urogenital tract. The objective of this study is to analyze the morphological modifications in the prostate ventral lobe of pubertal and adult rats chronically treated with AAS, using morphometric methods. Materials and Methods: We studied 39 male Wistar rats weighing between 400 g and 550 g. The rats were divided into four groups: (a) control rats, with 105 days of age (C105) (n = 7); (b) control rats with 65 days of age (C65) (n = 9), injected only with the vehicle (peanut oil); (c) treated rats, with 105 days of age (T105) (n = 10) and (d) treated rats with 65 days of age (T65) (n = 13). The treated rats were injected with nandrolone decanoate at a dose of 10 mg.Kg-1 body weight. The steroid hormone and the vehicle were administered by intramuscular injection once a week for eight weeks. The rats were killed at 161 days of age (C105 and T105) and 121 days of age (C65 and T65) and the ventral prostate lobe was dissected and processed for histology. The height of the acinar epithelium, the surface densities of the lumen, epithelium and stroma were observed with X400 magnification using an Olympus light microscope coupled to a Sony CCD video camera, and the images transferred to a Sony monitor KX14-CP1. The selected histological areas were then quantified using the M42 test-grid system on the digitized fields. The data were analyzed with the Graphpad software. To compare the quantitative data in both groups (controls and treated) and the outcomes, Student's t-test was used (p < 0.05 was considered significant). Results: The weight (p < 0.001) and volume (p = 0.004) of the prostate ventral lobe showed differences between C65 and T65 groups and between C105 and T105 groups. The epithelium height showed no difference between groups C65 and T65 (p = 0.8509), but the T105 group showed an increase of 32% compared to C105 (p = 0.0089). Concerning the lumen, surface density presented no difference between C65 and T65 (p = 0.9031) and a decrease of 19% for T105 compared to C105 (p = 0.0061). There was no difference in epithelium surface density between C65 and T65 (p = 0.7375), but it was 51% higher (p = 0.0065) in T105 compared with C105. Regarding stroma surface density, there were no differences between C65 and T65 or between C105 and T105. Finally, there was no difference in collagen pattern between C105 and T105, but T65 showed a predominance of collagen fibers compared to C65. Conclusion: The use of anabolic androgenic steroids in rats promotes structural changes in the prostate. We observed structural changes in the weight, volume and epithelium height of the prostate ventral lobe and a predominance of collagen fibers.

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“…The literature also shows that anabolic steroids induce testicular atrophy (23) and increase the weights of the prostate and seminal vesicles (24,25). Supporting these findings, in the present study nandrolone treatment induced gains in seminal vesicle and prostate weight as well as a decrease in testicular weight, confirming the effectiveness of the treatment protocol used.…”

Section: Effectiveness Of the Experimental Protocolsmentioning

confidence: 99%

Vascular adaptive responses to physical exercise and to stress are affected differently by nandrolone administration

Bruder‐Nascimento

Cordellini

2011

Braz J Med Biol Res

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High doses of nandrolone decanoate reduce volume of testis and length of seminiferous tubules in rats

Cited by 41 publications

References 16 publications

Stereological estimation of ovarian oocyte volume, surface area and number: application on mice treated with nandrolone decanoate

Stereological estimation of ovarian oocyte volume, surface area and number: application on mice treated with nandrolone decanoate

The prostate after administration of anabolic androgenic steroids: a morphometrical study in rats

Vascular adaptive responses to physical exercise and to stress are affected differently by nandrolone administration

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