High-dose vitamin C and cancer

Ünlü, Ahmet; Kirca, Onder; Özdoğan, Mustafa; Nayır, Erdinç

doi:10.1016/j.jons.2015.11.010

Cited by 44 publications

(35 citation statements)

References 28 publications

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“…Ascorbic acid (AA) is synthesized from D-glucuronate [1]. In the AA synthesis pathway, L-gulonate is converted to L-gulonolactone by a lactonase called SMP30, whose absence leads to AA deficiency in mice [23].…”

Section: Discussionmentioning

confidence: 99%

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Ascorbic Acid Attenuates Acute Ethanol-Induced Liver Injury in SMP30 Knockout Mice

Cho¹,

Ullah²,

Elfadl³

et al. 2017

FNS

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Ascorbic acid (AA) is recognized as a free radical scavenger that protects cells from oxidative stress-induced damage. However, no studies have investigated the role of AA in acute alcoholic liver disease using senescence marker protein-30 (SMP30) knockout (KO) mice. SMP30 is a novel 34-kDa protein involved in AA biosynthesis. The present study aimed to elucidate the physiological functions of AA in acute ethanol-induced liver injury using SMP30 KO mice, which cannot synthesize AA in vivo. After a 4-week experimental period, mice were divided into six groups. The following three groups comprised the ethanol treatment groups: WT-E group (wild-type), KV-E group (AA-supplemented), and KT-E group (AA-deficient). Mice were exposed to an acute dose of ethanol (6 g ethanol/kg) administered by gavage once a day for three days. The other three control groups, namely, WT-C, KV-C, and KT-C control groups, received an equal volume of water via oral administration. Analysis of changes in body weight showed that mice in the KT-E group had significant loss of body weight compared to the control, KV-E, and WT-E groups. Behavioral analysis revealed that alcohol exposure significantly increased alcohol sensitivity in the KT-E group, whereas the WT-E, KV-E, and control groups developed ethanol tolerance. Aspartate transaminase (AST) levels in the KT-E group were significantly higher than those in the control, KV-E, and WT-E groups. The number of large and binucleated hepatocytes was significantly higher in the KT-E group than in the KV-E and WT-E groups. In addition, cytochrome P450 2E1 (CYP2E1) was over expressed in the central vein in the KT-E group when compared to the KV-E and WT-E groups. Our current findings indicate that AA supplementation in SMP30 KO mice can alleviate alcohol-induced liver damage by down regulating CYP2E1 expression. These results suggest that reduced CYP2E1 expression is a novel How to cite this paper:

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Section: Discussionmentioning

confidence: 99%

“…Ascorbic acid (AA) is widely known as a water-soluble antioxidant that plays a major role in preventing cellular damage caused by free radicals, highly reactive species leading to cell damage and homeostatic disruption [1]. Various studies have been conducted to investigate the anti-oxidative properties of AA.…”

Section: Introductionmentioning

confidence: 99%

Ascorbic Acid Attenuates Acute Ethanol-Induced Liver Injury in SMP30 Knockout Mice

Cho¹,

Ullah²,

Elfadl³

et al. 2017

FNS

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“…High dose Vitamin C in cancer treatment is being debated for a long time (Unlu et al, 2016) but there is a little evidence how does Vitamin C acts against cancer cells. In recent days, there is an increased interest in studying Vitamin C as an adjunct treatment with chemotherapy (Hoffer et al, 2015;Fritz et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Vitamin C retards the growth and motility of liver cancer cells huh 7 in wound healing assay

2017

Int. J. Biosci.

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Vitamin C has long been discussed as having anticancer potential but with little evidence on mechanism of action. Some clinical studies have been performed but with no conclusive results. Researchers have been calling for more studies. The aim of study was to investigate the anti-cancer role of high dose Vitamin C on growth and motility of Liver cancer cells Huh 7 in wound healing Assay. Huh-7 cells were cultured in complete medium (DMEM) at 37 o C with 5%

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“…However, researchers now suggest that vitamin C prevents cancer by neutralizing ROS before they can damage DNA and initiate tumor growth. Furthermore, it has been proposed that vitamin C may also act as a pro-oxidant, helping the body's own ROS destroy early-stage tumors [27,28]. Currently, the recommended dietary allowance (RDA) in many countries ranges from 40-90 mg/day, although the results of various studies suggesting that the protective vitamin C concentrated in plasma for the minimum risk of free radical diseases corresponds to an intake of 124.2 mg/day (in the range of 92-181 mg) [10,29].…”

Section: Protective Efects Of Vitamin C Against Genotoxic Damage Frommentioning

confidence: 99%

The Role of Vitamin C in the Protection and Modulation of Genotoxic Damage Induced by Metals Associated with Oxidative Stress

2017

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This chapter reviews the efects of vitamin C on metal-induced genotoxicity. By focusing on cuting-edge studies, including our own results in experiments with vanadium(V) and chromium(VI), the suggestion that vitamin C can be used efectively to protect against or reduce the genotoxic efects induced by metal exposure by suppressing oxidative stress is particularly explored. After explaining the chemical mechanisms involved in oxidative stress associated with heavy metals, this chapter discusses the various proposals regarding the physiological processes of vitamin C at the molecular level, its relationship with oxidative stress, levels of 8-hydroxydeoxyguanosine 7, and apoptosis, and its role in the protection and modulation of DNA damage, as well as how they it with our own results that showed an increase in apoptosis and 8-OH-dG when vitamin C was administered in addition to the metallic compounds. The relevant gaps in our understanding of the role of vitamin C with regard to these issues are highlighted, as well as the key importance of its clinical use, and ultimately, human health.

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High-dose vitamin C and cancer

Cited by 44 publications

References 28 publications

Ascorbic Acid Attenuates Acute Ethanol-Induced Liver Injury in SMP30 Knockout Mice

Ascorbic Acid Attenuates Acute Ethanol-Induced Liver Injury in SMP30 Knockout Mice

Vitamin C retards the growth and motility of liver cancer cells huh 7 in wound healing assay

The Role of Vitamin C in the Protection and Modulation of Genotoxic Damage Induced by Metals Associated with Oxidative Stress

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