High-dose of vitamin D supplement is associated with reduced susceptibility of monocyte-derived macrophages to dengue virus infection and pro-inflammatory cytokine production: An exploratory study

Giraldo, Diana M.; Cardona, Andrés; Urcuqui-Inchima, Silvio

doi:10.1016/j.cca.2017.12.044

Cited by 50 publications

(45 citation statements)

References 54 publications

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“…This is in close agreement with previous reports in mice that TBK1 is required for the activation of innate immune responses to RNA viruses [41,42]. The VD, a well-known steroid hormone that regulates innate immune response, plays a vital role in anti-viral infection [15,16,46]. We have shown that VD decreased the fecal consistency scores of RV-challenged pigs, which have powerful anti-RV effects in vivo and in vitro [9,17,18].…”

Section: Discussionsupporting

confidence: 92%

Vitamin D Alleviates Rotavirus Infection through a Microrna-155-5p Mediated Regulation of the TBK1/IRF3 Signaling Pathway In Vivo and In Vitro

Zhao

Ran

et al. 2019

IJMS

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(1) Background: Vitamin D (VD) plays a vital role in anti-viral innate immunity. However, the role of VD in anti-rotavirus and its mechanism is still unclear. The present study was performed to investigate whether VD alleviates rotavirus (RV) infection through a microRNA-155-5p (miR-155-5p)-mediated regulation of TANK-binding kinase 1 (TBK1)/interferon regulatory factors 3 (IRF3) signaling pathway in vivo and in vitro. (2) Methods: The efficacy of VD treatment was evaluated in DLY pig and IPEC-J2. Dual-luciferase reporter activity assay was performed to verify the role of miR-155-5p in 1α,25-dihydroxy-VD3 (1,25D3) mediating the regulation of the TBK1/IRF3 signaling pathway. (3) Results: A 5000 IU·kg–1 dietary VD3 supplementation attenuated RV-induced the decrease of the villus height and crypt depth (p < 0.05), and up-regulated TBK1, IRF3, and IFN-β mRNA expressions in the jejunum (p < 0.05). Incubation with 1,25D3 significantly decreased the RV mRNA expression and the RV antigen concentration, and increased the TBK1 mRNA and protein levels, and the phosphoprotein IRF3 (p-IRF3) level (p < 0.05). The expression of miR-155-5p was up-regulated in response to an RV infection in vivo and in vitro (p < 0.05). 1,25D3 significantly repressed the up-regulation of miR-155-5p in vivo and in vitro (p < 0.05). Overexpression of miR-155-5p remarkably suppressed the mRNA and protein levels of TBK1 and p-IRF3 (p < 0.01), while the inhibition of miR-155-5p had an opposite effect. Luciferase activity assays confirmed that miR-155-5p regulated RV replication by directly targeting TBK1, and miR-155-5p suppressed the TBK1 protein level (p < 0.01). (4) Conclusions: These results indicate that miR-155-5p is involved in 1,25D3 mediating the regulation of the TBK1/IRF3 signaling pathway by directly targeting TBK1.

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Section: Discussionsupporting

confidence: 92%

Vitamin D Alleviates Rotavirus Infection through a Microrna-155-5p Mediated Regulation of the TBK1/IRF3 Signaling Pathway In Vivo and In Vitro

Zhao

Ran

et al. 2019

IJMS

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“…In line with findings from other reports (10), our observations that vitamin D-derived antiviral mechanisms can restrict viral infection and attenuate the pro-inflammatory response (22) have been corroborated ex vivo in two different vitamin D supplementation exploratory studies. We demonstrated that a daily oral supplement of 4000 IU of vitamin D during 10 days represented an adequate dose to enhance dengue virus control and reduce the cytokine response, in vitro, suggesting that vitamin D status can, in fact, restrict the viral assault (53,54). Accordingly, several studies have highlighted the beneficial role of vitamin D sufficiency levels and supplementation for viral respiratory infections (55)(56)(57).…”

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confidence: 92%

Vitamin D Supplementation: A Potential Approach for Coronavirus/COVID-19 Therapeutics?

Arboleda

Urcuqui-Inchima

2020

Front. Immunol.

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“…This might explain the difference in findings. Another study conducted by Giraldo et al reported high-dose vitamin D supplementation to be associated with reduced susceptibility of monocyte derived macrophages to dengue virus infection and pro-inflammatory cytokine production [29]. In contrast, our study group consisted of dengue patients who did not receive any vitamin D supplements, since we wanted to assess the role of existing level of vitamin D in these patients.…”

Section: Discussionmentioning

confidence: 99%

Levels of 25-hydroxy Vitamin D3 and Vitamin D Receptor Polymorphism in Severe Dengue Cases from New Delhi

et al. 2020

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Background: Dengue is the “phoenix” that never went to ashes. First identified in 1943, in Japan, dengue virus has worldwide distribution and is a grave public health concern in developing countries like India; Methods: A cross sectional study was conducted among adults suspected of having dengue fever and attending Lok Nayak Hospital, New Delhi. Restriction Fragment Length Polymorphism was completed for the detection of vitamin D receptor (VDR) gene polymorphism; Results: Serum 25-hydroxy vitamin D3 (vitamin D) levels were found to be 1.6 times elevated in severe dengue cases as compared to healthy controls. Vitamin D levels were significantly higher in secondary infections compared to primary infections as well as secondary severe dengue cases as compared to secondary non-severe cases (p value < 0.05). A significant association of the T allele (rs2228570) was seen in severe dengue cases, while, when comparing the A/A with A/C and C/C genotypes (rs7975232) among dengue cases and healthy controls, the odds ratio was estimated to be 1.24 (0.55–2.75, p > 0.05) and 0.28 (0.08–0.96, p < 0.05) respectively; Conclusions: The present study is an attempt at decoding the role of vitamin D in dengue disease pathogenesis and exploring the role of genetic polymorphism in dengue disease pathogenesis.

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High-dose of vitamin D supplement is associated with reduced susceptibility of monocyte-derived macrophages to dengue virus infection and pro-inflammatory cytokine production: An exploratory study

Cited by 50 publications

References 54 publications

Vitamin D Alleviates Rotavirus Infection through a Microrna-155-5p Mediated Regulation of the TBK1/IRF3 Signaling Pathway In Vivo and In Vitro

Vitamin D Alleviates Rotavirus Infection through a Microrna-155-5p Mediated Regulation of the TBK1/IRF3 Signaling Pathway In Vivo and In Vitro

Vitamin D Supplementation: A Potential Approach for Coronavirus/COVID-19 Therapeutics?

Levels of 25-hydroxy Vitamin D3 and Vitamin D Receptor Polymorphism in Severe Dengue Cases from New Delhi

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