High-dose immunoglobulin preparations improve survival in a CLP-induced rat model of sepsis

Yoshikawa, Takahisa; Takeuchi, Hiroya; Suda, Koichi; Miyasho, Taku; Yamada, Shingo; Okamoto, Minoru; Kawamura, Yoshio; Maruyama, Ikuro; Kitajima, Masaki; Kitagawa, Yuko

doi:10.1007/s00423-011-0878-4

Cited by 9 publications

(7 citation statements)

References 34 publications

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“…The data in the present study also demonstrated that IVIG treatment reduces the levels of two pro-inflammatory cytokines (TNF-α, and IL-6). This findings is consistent with previous studies 10,11,34 . In our study, the degree of cytokine reduction was not necessarily dependent on the IVIG dose.…”

Section: Hagiwara Et Al and Yoshikawa Et Al Have Suggested That Immsupporting

confidence: 94%

“…The Fab-dependent effects include neutralization of bacteria, neutralization of bacterial toxins, neutralization of bacterial superantigens, suppression of cytokines produced by immune cells, and autoantibodies 29 . Previous studies have also shown IVIG has an anti-inflammatory effect 10,11,[30][31][32][33] . Thus, immunoglobulins could theoretically improve survival in our mouse sepsis model not only through direct antibacterial effects but also by exerting anti-inflammatory effects.…”

Section: Discussionmentioning

confidence: 89%

“…Physicians use intravenous immunoglobulin (IVIG) to treat several disease, such as severe infection, agammaglobulinemia, Kawasaki disease and idiopathic thrombocytopenic purpura (ITP) 9 . Kawasaki disease and ITP are treated with 2000 mg/kg IVIG, and such a high-dose of IVIG has been shown to improve survival in animal model of sepsis 10,11 . Also in the clinic, Turgeon et al have reported a meta-analysis showing high-dose IVIG is effective for treating sepsis 12 .…”

Section: Introductionmentioning

confidence: 99%

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Intravenous Immunoglobulin Attenuates Cecum Ligation and Puncture-Induced Acute Lung Injury by Inhibiting Apoptosis of Alveolar Epithelial Cells

2020

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Section: Hagiwara Et Al and Yoshikawa Et Al Have Suggested That Immsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

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Intravenous Immunoglobulin Attenuates Cecum Ligation and Puncture-Induced Acute Lung Injury by Inhibiting Apoptosis of Alveolar Epithelial Cells

2020

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“…In recent years, immunoglobulin preparations have been utilized in patients with severe sepsis or septic shock (NorrbyTeglund et al, 2006;Yoshikawa et al, 2012). IgM-enriched IVIG contains Ig M, Ig A, and Ig G and it was shown that IgM-enriched IVIG (250 mg/kg) is more efficient than standard IgG IVIG in terms of toxin neutralization and bacterial agglutination in cases with superantigen-mediated sepsis (Darenberg et al, 2004;Norrby-Teglund et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

The protective effects of IgM-enriched immunoglobulin and erythropoietin on the lung and small intestine tissues of rats with induced sepsis: Biochemical and histopathological evaluation

Ateş

Doğan

Aksoy

et al. 2014

Pharmaceutical Biology

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Context: Sepsis continues to be a significant problem for critical care patients. Objective: To evaluate the protective effects of IgM-enriched immunoglobulin and erythropoietin on pulmonary and small intestine tissues in a rat model of intra-abdominal sepsis induced via the cecal ligation and puncture (CLP) method. Materials and methods: Male Sprague-Dawley rats were used. Control group (n ¼ 6): surgical procedure was not performed. Laparotomy was only performed in the sham group (n ¼ 6) and CLP was only performed in the sepsis (CLP) group (n ¼ 30). After erythropoietin (2000 U/kg, intraperitoneal) was given in the sepsis + erythropoietin (CLP + EPO) group (n ¼ 30), IgMenriched immunoglobulin (600 mg/kg, intraperitoneal) was given in the sepsis + pentaglobin (CLP + PEN) group (n ¼ 30), CLP was created. Intracardiac blood samples were collected for biochemical analysis; lung and small intestine tissue samples were removed for histopathological evaluation. Results: Plasma TNF-a levels (pg/ml) were similar among CLP, CLP + EPO, and CLP + PEN groups (204.0 ± 52.4, 198.5 ± 17.3, and 214.6 ± 93.6, respectively). The CLP group had higher plasma IL-1b levels (pg/ml) compared with CLP + EPO and CLP + PEN groups (325.1 ± 134.1, 164.3 ± 25.6, and 186.3 ± 26.0, respectively) (p50.05). Rats in CLP + EPO and CLP + PEN groups had abolished histopathologic appearance of lung and small intestine tissues compared with rats in the CLP group. Discussion and conclusion: Our findings support the use of EPO and IgM-enriched immunoglobulin in the prevention of lung and small intestine injuries associated with sepsis.

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“…Gao et al (2012) have noted a significant increase of aST, alT, lDH, CK and creatinine serum level 12 hours from the Clp procedure in rats. Yoshikawa et al (2012) demonstrated that treatment with a high-dose of human immunoglobulin (1.500 mg/kg) significantly improves the survival of rats in the Clp model. based on correlation analysis of measured parameters, it was concluded that body temperature (T), blood urea nitrogen (bUN), creatinine, alT, CK, Il-6 and mCp-1 levels after the Clp were negatively correlated with survival, while platelet count (plT), Hmgb1 and granulocyte-macrophage colony stimulating factor (gmCCf) were positively correlated with the survival outcome.…”

Section: Resultsmentioning

confidence: 66%

Examination of enzymes concentration in the blood of rats with sepsis caused by mixed and pure bacterial cultures

Dragica¹,

Kovačević²,

Zekic³

et al. 2013

ACTA VET-BEOGRAD

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A clinical form of sepsis induced by cecal ligation and puncture (CLP) was caused in order to monitor the concentration of enzymes (alanine aminotransferase -ALT, aspartate aminotransferase -AST, lactate dehydrogenase -LDH, amylase and creatine kinase -CK) in the rat blood. Experiments were performed on male Wistar rats, weighing on average 215±25 g. The rats were divided into four groups. In the first three groups (n=28 per group), sepsis was induced by pure culture of Escherichia coli (EC) or Staphylococcus aureus (SA) and mixed culture (MK) of caecum, while the fourth group included 20 control rats who underwent an abdominal incision. Blood was taken in time intervals of 12, 24, 72 and 120 hours.During the experimental protocol, we identified significant changes of all monitored enzymes in the serum of infected rats. After a period of 12 hours there was a significant increase in ALT (all rats with sepsis), AST and LDH (rats in the MK group) levels, while a decrease was noted in the concentration of amylase (EC, SA). Similarly, 24 hours after the CLP procedure, a significant decrease of amylase (MK) and AST (SA) was recorded, while serum LDH level varied significantly from elevated (EC, SA) to reduced (MC) values. Finally, at the time intervals of 72 and 120 hours the concentration of nearly all monitored enzymes has shown a decline, while significance was noted in lowering of ALT (MK), AST (SA, EC) and amylase (SA) levels. Statistical significance could not be observed in the change of CK levels at any of examined time points.

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High-dose immunoglobulin preparations improve survival in a CLP-induced rat model of sepsis

Abstract: Our results suggest that high-dose immunoglobulin therapy may improve the serum endotoxin and HMGB1 levels and overall survival rate in sepsis by inhibiting the inflammation.

Cited by 9 publications

References 34 publications

Intravenous Immunoglobulin Attenuates Cecum Ligation and Puncture-Induced Acute Lung Injury by Inhibiting Apoptosis of Alveolar Epithelial Cells

Intravenous Immunoglobulin Attenuates Cecum Ligation and Puncture-Induced Acute Lung Injury by Inhibiting Apoptosis of Alveolar Epithelial Cells

The protective effects of IgM-enriched immunoglobulin and erythropoietin on the lung and small intestine tissues of rats with induced sepsis: Biochemical and histopathological evaluation

Examination of enzymes concentration in the blood of rats with sepsis caused by mixed and pure bacterial cultures

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