High Diversity of <i>Ligularia dictyoneura</i> in Chemical Composition and DNA Sequence

Nagano, Hajime; Iwazaki, Yukiko; Matsushima, Mika; Satô, Masahiko; Gong, Xun; Shen, Yuemao; Harada, Hiroshi; Kuroda, Chiaki; Hanai, Ryo

doi:10.1002/cbdv.200790237

Cited by 35 publications

(32 citation statements)

References 44 publications

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“…Because the variation in L. latihastata is fairly large and because no intermediate between the two types of the sequence is found, the diversity is likely to result from hybridization. Actually, the majority sequence type, with 245T, 301T, 409T, and 469C, has been found in other Ligularia species in the section Ligularia, and in L. dictyoneura [22]. Further molecular, cytological, and morphological studies are necessary to determine whether L. latihastata is a product of recent hybridization speciation or if the multiple atpB-rbcL types have resulted from introgression [23].…”

Section: Chemical and Genetic Diversity Of Ligularia Latihastata And mentioning

confidence: 95%

Chemical and Genetic Diversity of Ligularia latihastata and Ligularia villosa in Yunnan Province of China

Kuroda

Kiuchi

Torihata

et al. 2007

Chemistry & Biodiversity

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The chemical constituents of the root extracts and the nucleotide sequences of the atpB-rbcL intergenic region of Ligularia latihastata and L. villosa, collected in northwestern Yunnan Province, were studied. In the twelve collected samples of L. latihastata, two major benzofurans, 5,6-dimethoxy-2-(1-methylethenyl)-1-benzofuran (1) and euparin (2) were detected as major components. The minor compound (2R*,3S*)-5-acetyl-2,3-dihydro-6-hydroxy-2-(1-methylethenyl)-1-benzofuran-3-yl (2Z)-2-[(acetoxy)methyl]but-2-enoate (4) was found to be susceptible to artifact formation upon extraction with EtOH. The intra-specific diversity in chemical composition of the samples was small, but the diversity in the atpB-rbcL sequence was fairly large. Compounds 1 and 2 were also found in the three collected samples of L. villosa, indicating that the two species are chemically close to each other, in agreement with morphological taxonomy.

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Section: Chemical and Genetic Diversity Of Ligularia Latihastata And mentioning

confidence: 95%

Chemical and Genetic Diversity of Ligularia latihastata and Ligularia villosa in Yunnan Province of China

Kuroda

Kiuchi

Torihata

et al. 2007

Chemistry & Biodiversity

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“…There were two major spots (R f (hexane/AcOEt 7 : 3) 0.61 and 0.55) together with spots of minor components, when detected by coloring with MoO 3 / H 3 PO 4 .…”

Section: Chemical and Genetic Study Of Ligularia Anoleuca And L Veitmentioning

confidence: 99%

“…-In this study, furanoeremophilane derivatives 1 [4], 2 [20], and 3 were isolated from L. anoleuca. From L. veitchiana, euparin (6) [22] and its derivatives 7 [23] and 8 [24] were obtained.…”

Section: Chemical and Genetic Study Of Ligularia Anoleuca And L Veitmentioning

confidence: 99%

“…After further purification with GLASSMILK (Qbiogene), the DNA was used as template for polymerase chain reaction (PCR) with HotStarTaq plus polymerase (QIAGEN). The primers used for the amplification of the ITS1 -5.8S-ITS2 and the atpB-rbcL regions were as reported in [36]. PCR Products were subjected to electrophoresis in an agarose slab and purified with High Pure PCR Product Purification Kit (Roche Diagnostics).…”

Section: Experimental Partmentioning

confidence: 99%

“…PCR Products were subjected to electrophoresis in an agarose slab and purified with High Pure PCR Product Purification Kit (Roche Diagnostics). Sequencing reactions were carried out with BigDye Terminator v.3.1 Cycle Sequencing Kit (Applied Biosystems) and the primers reported in [36]. The reactions were analyzed on a 3100xl Genetic Analyzer (Applied Biosystems).…”

Section: Experimental Partmentioning

confidence: 99%

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Chemical and Genetic Study of Ligularia anoleuca and L. veitchiana in Yunnan and Sichuan Provinces of China

Nagano¹,

Kanda²,

Yamada³

et al. 2010

Helvetica Chimica Acta

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Chemical and genetic study of Ligularia anoleuca and L. veitchiana, which belong to section Ligularia, series Speciosae, was carried out. From L. anoleuca samples, collected in Yunnan and Sichuan Provinces of China, a new compound, furanoeremophil-1(10)-en-6a-ol, was isolated together with known 6b-{[2-(hydroxymethyl)prop-2-enoyl]oxy}furanoeremophil-1(10)-ene and 1b,10b-epoxy-6b-{[2-(hydroxymethyl)prop-2-enoyl]oxy}furanoeremophilane. From L. veitchiana samples, collected in Yunnan Province, euparin, 2-isopropenyl-5,6-dimethoxybenzofuran, and 6-hydroxy-3b-methoxytrementone were isolated. DNA Sequencing of the internal transcribed spacers of the ribosomal RNA gene showed that the two species are not particularly close despite morphological similarities, in agreement with the chemical results.Introduction. -Ligularia Cass. (Asteraceae) in the Hengduan Mountains of China is highly diversified [1 -3], and suitable for the study of diversity and evolution of plant chemicals. We have been investigating the chemical diversity of Ligularia species in combination with genetic analysis [4 -11]. To examine the chemical diversity, furanoeremophilanes and related compounds are analyzed, since the presence of the former can be easily detected by Ehrlichs test on TLC plates [12]. To examine the genetic diversity, the DNA sequences of the two internal transcribed spacers (ITSs) of the ribosomal RNA gene in the nuclear genome and the atpB-rbcL intergenic region in the plastid genome are analyzed [13]. To date, we have revealed intra-specific diversity in a number of species in Yunnan and Sichuan Provinces. For example, L. dictyoneura

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Chemical and Genetic Differentiation of Ligularia hodgsonii in Japan and China

Torihata

Chao

Kuroda

et al. 2009

Chemistry & Biodiversity

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The chemical composition of the root extract and neutral base sequences of L. hodgsonii samples collected in Japan and China were examined. From the Japanese and the Chinese samples, 6beta-(angeloyloxy)furanoeremophilan-15-oic acid and 3beta-acetoxy-6beta-[(2-methylbutanoyl)oxy]furanoeremophilan-10beta-ol were isolated as the major component, respectively. The sequence of the internal transcribed spacers (ITSs) of the ribosomal RNA gene was different in the Japanese and the Chinese samples. In contrast to the significant genetic difference, chemical diversity was limited to the positions of O-functionalities on the furanoeremophilane skeleton, supporting our hypothesis that the production of furanoeremophilanes is ecologically advantageous.

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High Diversity of Ligularia dictyoneura in Chemical Composition and DNA Sequence

Cited by 35 publications

References 44 publications

Chemical and Genetic Diversity of Ligularia latihastata and Ligularia villosa in Yunnan Province of China

Chemical and Genetic Diversity of Ligularia latihastata and Ligularia villosa in Yunnan Province of China

Chemical and Genetic Study of Ligularia anoleuca and L. veitchiana in Yunnan and Sichuan Provinces of China

Chemical and Genetic Differentiation of Ligularia hodgsonii in Japan and China

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